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Originally published In Press as doi:10.1074/jbc.M209827200 on December 6, 2002

J. Biol. Chem., Vol. 278, Issue 8, 6093-6100, February 21, 2003
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Mcm1 Binds Replication Origins*

Victoria K. ChangDagger , Michael J. Fitch§, Justin J. Donato§||, Tim W. Christensen§, A. Margaret Merchant§, and Bik K. Tye**

From the Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853-2703 and the Dagger  Department of Chemistry, Drew University, Madison, New Jersey 07940

Mcm1 is an essential protein required for the efficient replication of minichromosomes and the transcriptional regulation of early cell cycle genes in Saccharomyces cerevisiae. In this study, we report that Mcm1 is an abundant protein that associates globally with chromatin in a punctate pattern. We show that Mcm1 is localized at replication origins and plays an important role in the initiation of DNA synthesis at a chromosomal replication origin in vivo. Using purified Mcm1 protein, we show that Mcm1 binds cooperatively to multiple sites at autonomously replicating sequences. These results suggest that, in addition to its role as a transcription factor for the expression of replication genes, Mcm1 may influence the local structure of replication origins by direct binding.

* This work is supported in part by National Institutes of Health Grant GM34190 (to B. K. T.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Supported by National Institutes of Health Training Grants GM07273 or GM07616.

Present address: Scripps Research Institute, La Jolla, CA 92037.

|| GAANN fellow.

** To whom correspondence should be addressed: Dept. of Molecular Biology and Genetics, Cornell University, 325 Biotechnology Bldg., Ithaca, NY 14853-2703. E-mail: bt16@cornell.edu.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
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