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J. Biol. Chem., Vol. 278, Issue 9, 7422-7430, February 28, 2003
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From the Division of Host-Parasite Interaction, Department of
Microbiology and Immunology, Institute of Medical Science,
University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
The human adrenal carcinoma cell line,
SW13, has been reported to be deficient in both BRG1 and
Brm expression and therefore is considered to lack a
functional SWI/SNF complex. We found that the original cell line
of SW13 is composed of two subtypes, one that expresses neither BRG1
nor Brm (SW13(vim
SW13 Cells Can Transition between Two Distinct
Subtypes by Switching Expression of BRG1 and
Brm Genes at the Post-transcriptional Level*
)) and the another, which does express both
(SW13(vim+)). The presence of BRG1 and Brm in SW13 correlates
completely with the cellular ability to express such genes as
vimentin, collagenase, c-met, and
CD44 that were under the control of a transcription factor,
AP-1, which was shown previously to require a functional SWI/SNF
complex for its transactivating activity. Transient treatment with
inhibitors of histone deacetylase induced a stable transition of
SW13(vim) to a cell type indistinguishable from SW13(vim+),
suggesting that these two subtypes are epigenetically different. Run-on
analysis indicated that, unlike these four genes driven by AP-1,
transcription of the BRG1 and Brm genes in
SW13(vim) are initiated at a frequency comparable with
SW13(vim+). In both SW13(vim) and SW13(vim+) cells, the
BRG1 and Brm genes were transcribed through the
entire gene at a similar efficiency, indicating that their expression was completely suppressed at the post-transcriptional level in SW13(vim) cells. We would like to propose that SW13 can spontaneously transition between two subtypes by switching expression of
BRG1 and Brm at the post-transcriptional level.
*
This work was supported in part by a grant-in-aid for
scientific research on priority areas from the Ministry of Education, Science, and Culture of Japan.The costs of publication of this article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 81-3-5449-5730;
Fax: 81-3-5449-5449; E-mail: iba@ims.u-tokyo.ac.jp.
Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
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