|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 278, Issue 9, 7607-7616, February 28, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Departments of There is currently limited data available
pertaining to the global characterization of the cell surface
proteome. We have implemented a strategy for the comprehensive
profiling and identification of surface membrane proteins. This
strategy has been applied to cancer cells, including the SH-SY5Y
neuroblastoma, the A549 lung adenocarcinoma, the LoVo colon
adenocarcinoma, and the Sup-B15 acute lymphoblastic leukemia (B cell)
cell lines and ovarian tumor cells. Surface membrane proteins of
viable, intact cells were subjected to biotinylation then
affinity-captured and purified on monomeric avidin columns. The
biotinylated proteins were eluted from the monomeric avidin columns as
intact proteins and were subsequently separated by two-dimensional
PAGE, transferred to polyvinylidene difluoride membranes, and
visualized by hybridization with streptavidin-horseradish peroxidase.
Highly reproducible, but distinct, two-dimensional patterns consisting
of several hundred biotinylated proteins were obtained for the
different cell populations analyzed. Identification of a subset of
biotinylated proteins among the different cell populations analyzed
using matrix-assisted laser desorption ionization and tandem mass
spectrometry uncovered proteins with a restricted expression pattern in
some cell line(s), such as CD87 and the activin receptor type IIB. We
also identified more widely expressed proteins, such as CD98, and a
sushi repeat-containing protein, a member of the selectin family.
Remarkably, a set of proteins identified as chaperone proteins were
found to be highly abundant on the cell surface, including GRP78,
GRP75, HSP70, HSP60, HSP54, HSP27, and protein disulfide isomerase.
Comprehensive profiling of the cell surface proteome provides an
effective approach for the identification of commonly occurring
proteins as well as proteins with restricted expression patterns in
this compartment.
Global Profiling of the Cell Surface Proteome of
Cancer Cells Uncovers an Abundance of Proteins with Chaperone
Function*
,,,,§,,,,,
, and
Pediatrics and
¶ Pathology, University of Michigan, Ann Arbor, Michigan
48109-0656
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of
Pediatrics, University of Michigan, 1150 West Medical Center Dr., Rm. A520 MSRB-1, Ann Arbor, MI 48109-0656. Tel.: 734-763-0917; Fax: 734-647-8148; E-mail: dmisek@umich.edu.
Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Li, J. Yu, Y. Wang, N. M. Griffin, F. Long, S. Shore, P. Oh, and J. E. Schnitzer Enhancing Identifications of Lipid-embedded Proteins by Mass Spectrometry for Improved Mapping of Endothelial Plasma Membranes in Vivo Mol. Cell. Proteomics, June 1, 2009; 8(6): 1219 - 1235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Mintz, M. Cardo-Vila, M. G. Ozawa, A. Hajitou, R. Rangel, L. Guzman-Rojas, D. R. Christianson, M. A. Arap, R. J. Giordano, G. R. Souza, et al. An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment PNAS, February 17, 2009; 106(7): 2182 - 2187. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ran, H. Hu, D. Hu, Z. Zhou, Y. Sun, L. Yu, L. Sun, J. Pan, J. Liu, T. Liu, et al. Derlin-1 Is Overexpressed on the Tumor Cell Surface and Enables Antibody-Mediated Tumor Targeting Therapy Clin. Cancer Res., October 15, 2008; 14(20): 6538 - 6545. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Conn, M. A. Madsen, B. F. Cravatt, W. Ruf, E. I. Deryugina, and J. P. Quigley Cell Surface Proteomics Identifies Molecules Functionally Linked to Tumor Cell Intravasation J. Biol. Chem., September 26, 2008; 283(39): 26518 - 26527. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yang, R. Rao, J. Shen, Y. Tang, W. Fiskus, J. Nechtman, P. Atadja, and K. Bhalla Role of Acetylation and Extracellular Location of Heat Shock Protein 90{alpha} in Tumor Cell Invasion Cancer Res., June 15, 2008; 68(12): 4833 - 4842. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Walsh, D. Whelan, A. Bielanowicz, B. Skinner, R. J. Aitken, M. K. O'Bryan, and B. Nixon Identification of the Molecular Chaperone, Heat Shock Protein 1 (Chaperonin 10), in the Reproductive Tract and in Capacitating Spermatozoa in the Male Mouse Biol Reprod, June 1, 2008; 78(6): 983 - 993. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. N. Wuppermann, K. Molleken, M. Julien, C. A. Jantos, and J. H. Hegemann Chlamydia pneumoniae GroEL1 Protein Is Cell Surface Associated and Required for Infection of HEp-2 Cells J. Bacteriol., May 15, 2008; 190(10): 3757 - 3767. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Shani, W. H. Fischer, N. J. Justice, J. A. Kelber, W. Vale, and P. C. Gray GRP78 and Cripto Form a Complex at the Cell Surface and Collaborate To Inhibit Transforming Growth Factor Signaling and Enhance Cell Growth Mol. Cell. Biol., January 15, 2008; 28(2): 666 - 677. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Townsend S-Glutathionylation: Indicator of Cell Stress and Regulator of the Unfolded Protein Response Mol. Interv., December 1, 2007; 7(6): 313 - 324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Mallikaratchy, Z. Tang, S. Kwame, L. Meng, D. Shangguan, and W. Tan Aptamer Directly Evolved from Live Cells Recognizes Membrane Bound Immunoglobin Heavy Mu Chain in Burkitt's Lymphoma Cells Mol. Cell. Proteomics, December 1, 2007; 6(12): 2230 - 2238. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Shamaei-Tousi, J. P. Halcox, and B. Henderson Stressing the obvious? Cell stress and cell stress proteins in cardiovascular disease Cardiovasc Res, April 1, 2007; 74(1): 19 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Schmitt, M. Gehrmann, M. Brunet, G. Multhoff, and C. Garrido Intracellular and extracellular functions of heat shock proteins: repercussions in cancer therapy J. Leukoc. Biol., January 1, 2007; 81(1): 15 - 27. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Sun, J. A. Ranish, A. G. Utleg, J. T. White, X. Yan, B. Lin, and L. Hood Shotgun Glycopeptide Capture Approach Coupled with Mass Spectrometry for Comprehensive Glycoproteomics Mol. Cell. Proteomics, January 1, 2007; 6(1): 141 - 149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Goplen, J. Wang, P. O. Enger, B. B. Tysnes, A.J.A. Terzis, O. D. Laerum, and R. Bjerkvig Protein disulfide isomerase expression is related to the invasive properties of malignant glioma. Cancer Res., October 15, 2006; 66(20): 9895 - 9902. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Stangl, A. Wortmann, U. Guertler, and G. Multhoff Control of Metastasized Pancreatic Carcinomas in SCID/Beige Mice with Human IL-2/TKD-Activated NK Cells J. Immunol., May 15, 2006; 176(10): 6270 - 6276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. K. Lamb, C. Mee, W. Xu, L. Liu, S. Blond, A. Cooper, I. G. Charles, and A. R. Hawkins The Affinity of a Major Ca2+ Binding Site on GRP78 Is Differentially Enhanced by ADP and ATP J. Biol. Chem., March 31, 2006; 281(13): 8796 - 8805. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. S. Son, J. H. Park, Y. K. Kang, J.-S. Park, H. S. Choi, J. Y. Lim, J. E. Lee, J. B. Lee, M. S. Ko, Y.-S. Kim, et al. Heat Shock 70-kDa Protein 8 Isoform 1 Is Expressed on the Surface of Human Embryonic Stem Cells and Downregulated upon Differentiation Stem Cells, October 1, 2005; 23(10): 1502 - 1513. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Pilzer and Z. Fishelson Mortalin/GRP75 promotes release of membrane vesicles from immune attacked cells and protection from complement-mediated lysis Int. Immunol., September 1, 2005; 17(9): 1239 - 1248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Pozza and R. C. Austin Getting a GRP on Tissue Factor Activation Arterioscler. Thromb. Vasc. Biol., August 1, 2005; 25(8): 1529 - 1531. [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Grzesiak, K. C. Smith, C. Chalberg, C. Truong, D. W. Burton, L. J. Deftos, and M. Bouvet Heat Shock Protein-70 Expressed on the Surface of Cancer Cells Binds Parathyroid Hormone-Related Protein in Vitro Endocrinology, August 1, 2005; 146(8): 3567 - 3576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W. Graner and D. D. Bigner Chaperone proteins and brain tumors: Potential targets and possible therapeutics Neuro-oncol, July 1, 2005; 7(3): 260 - 278. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Sinha, K. Kosalai, S. Arora, A. Namane, P. Sharma, A. N. Gaikwad, P. Brodin, and S. T. Cole Immunogenic membrane-associated proteins of Mycobacterium tuberculosis revealed by proteomics Microbiology, July 1, 2005; 151(7): 2411 - 2419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Gastpar, M. Gehrmann, M. A. Bausero, A. Asea, C. Gross, J. A. Schroeder, and G. Multhoff Heat Shock Protein 70 Surface-Positive Tumor Exosomes Stimulate Migratory and Cytolytic Activity of Natural Killer Cells Cancer Res., June 15, 2005; 65(12): 5238 - 5247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Johnson, T. T. T. Le, C. A. Dobbin, T. Banovic, C. B. Howard, F. d. M. L. Flores, D. Vanags, D. J. Naylor, G. R. Hill, and A. Suhrbier Heat Shock Protein 10 Inhibits Lipopolysaccharide-induced Inflammatory Mediator Production J. Biol. Chem., February 11, 2005; 280(6): 4037 - 4047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Korbelik, J. Sun, and I. Cecic Photodynamic Therapy-Induced Cell Surface Expression and Release of Heat Shock Proteins: Relevance for Tumor Response Cancer Res., February 1, 2005; 65(3): 1018 - 1026. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Asquith, A. J. Harman, E. A. McLaughlin, B. Nixon, and R. J. Aitken Localization and Significance of Molecular Chaperones, Heat Shock Protein 1, and Tumor Rejection Antigen gp96 in the Male Reproductive Tract and During Capacitation and Acrosome Reaction Biol Reprod, February 1, 2005; 72(2): 328 - 337. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Asquith, R. M. Baleato, E. A. McLaughlin, B. Nixon, and R. J. Aitken Tyrosine phosphorylation activates surface chaperones facilitating sperm-zona recognition J. Cell Sci., July 15, 2004; 117(16): 3645 - 3657. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Flores-Diaz, J.-C. Higuita, I. Florin, T. Okada, P. Pollesello, T. Bergman, M. Thelestam, K. Mori, and A. Alape-Giron A Cellular UDP-glucose Deficiency Causes Overexpression of Glucose/Oxygen-regulated Proteins Independent of the Endoplasmic Reticulum Stress Elements J. Biol. Chem., May 21, 2004; 279(21): 21724 - 21731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Gastpar, C. Gross, L. Rossbacher, J. Ellwart, J. Riegger, and G. Multhoff The Cell Surface-Localized Heat Shock Protein 70 Epitope TKD Induces Migration and Cytolytic Activity Selectively in Human NK Cells J. Immunol., January 15, 2004; 172(2): 972 - 980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Peirce, R. Wait, S. Begum, J. Saklatvala, and A. P. Cope Expression Profiling of Lymphocyte Plasma Membrane Proteins Mol. Cell. Proteomics, January 1, 2004; 3(1): 56 - 65. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Laragione, V. Bonetto, F. Casoni, T. Massignan, G. Bianchi, E. Gianazza, and P. Ghezzi Redox regulation of surface protein thiols: Identification of integrin {alpha}-4 as a molecular target by using redox proteomics PNAS, December 9, 2003; 100(25): 14737 - 14741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Reddy, C. Mao, P. Baumeister, R. C. Austin, R. J. Kaufman, and A. S. Lee Endoplasmic Reticulum Chaperone Protein GRP78 Protects Cells from Apoptosis Induced by Topoisomerase Inhibitors: ROLE OF ATP BINDING SITE IN SUPPRESSION OF CASPASE-7 ACTIVATION J. Biol. Chem., May 30, 2003; 278(23): 20915 - 20924. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |