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J. Biol. Chem., Vol. 279, Issue 1, 453-462, January 2, 2004

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The Yeast RNA-binding Protein Rbp1p Modifies the Stability of Mitochondrial Porin mRNA^*

Leh-Miauh Buu, Li-Ting Jang, and Fang-Jen S. Lee

From the Institute of Molecular Medicine, College of Medicine, and Department of Medical Research, National Taiwan University Hospital, National Taiwan University, 7 Chung Shan South Road, Taipei 100, Taiwan

The Saccharomyces cerevisiae RNA-binding protein Rbp1p was initially identified as a negative growth regulator; however, its function is still obscure. Here, we show that Rbp1p in cells is associated with structures that sediment at 10,000 as well as 100,000 x g. It appears microscopically as punctate signals partially localized to the perinuclear region. Over-expression of Rbp1p in yeast resulted in growth defects on nonfermentable carbon sources, suggesting a function for Rbp1p in mitochondrial biogenesis. Absence of Rbp1p increased the level of mitochondrial porin, whereas over-expression of Rbp1p, but not an N-terminally truncated form, decreased porin levels. Over-expression of Rbp1p also decreased the level of mitochondrial porin mRNA by enhancing its degradation, an effect that was dependent on all three of the Rbp1p RNA recognition motifs. In cells, the porin mRNA is associated with Rbp1p·RNP (ribonucleoprotein) complexes. In vitro binding assays showed that Rbp1p most likely interacts with a (C/G)U-rich element in the porin mRNA 3'-UTR. Based on these observations, we infer that Rbp1p has a role in negatively regulating mitochondrial porin expression post-transcriptionally.

Received for publication, August 21, 2003 , and in revised form, October 14, 2003.

^* This work was supported by Grants NHRI-GT-EX89S934L and NHRI-EX92-9222BI from the National Health Research Institutes, Taiwan, Republic of China (to F.-J. S. L.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: 886-2-2312-3456, ext. 5730; Fax: 886-2-2395-7801; E-mail: fangjen{at}ha.mc.ntu.edu.tw.

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