HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 10, 8879-8885, March 5, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/10/8879    most recent M309802200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Watanabe, I. Articles by Thornhill, W. B. Search for Related Content PubMed PubMed Citation Articles by Watanabe, I. Articles by Thornhill, W. B. Social Bookmarking                      What's this?

Glycosylation Affects the Protein Stability and Cell Surface Expression of Kv1.4 but Not Kv1.1 Potassium Channels

A PORE REGION DETERMINANT DICTATES THE EFFECT OF GLYCOSYLATION ON TRAFFICKING^*

Itaru Watanabe, Jing Zhu, Esperanza Recio-Pinto, and William B. Thornhill¶

From the Department of Biological Sciences, Fordham University, Bronx, New York, 10458 and the Department of Anesthesiology, New York University School of Medicine, New York, New York 10016

Kv1.1 and Kv1.4 potassium channels are plasma membrane glycoproteins involved in action potential repolarization. We have shown previously that glycosylation affects the gating function of Kv1.1 (Watanabe, I., Wang, H. G., Sutachan, J. J., Zhu, J., Recio-Pinto, E. & Thornhill, W. B. (2003) J. Physiol. (Lond.) 550, 51–66) and that a pore region determinant of Kv1.1 and Kv1.4 affects their cell surface trafficking negatively or positively, respectively (Zhu, J., Watanabe, I., Gomez, B. & Thornhill, W. B. (2001) J. Biol. Chem. 276, 39419–39427). Here we investigated the role of N-glycosylation of Kv1.1 and Kv1.4 on their protein stability, cellular localization pattern, and trafficking to the cell surface. We found that preventing N-glycosylation of Kv1.4 decreased its protein stability, induced its high partial intracellular retention, and decreased its cell surface protein levels, whereas it had little or no effect on these parameters for Kv1.1. Exchanging a trafficking pore region determinant between Kv1.1 and Kv1.4 reversed these effects of glycosylation on these chimeric channels. Thus it appeared that the Kv1.4 pore region determinant and the sugar tree attached to the S1–S2 linker showed some type of dependence in promoting proper trafficking of the protein to the cell surface, and this dependence can be transferred to chimeric Kv1.1 proteins that contain the Kv1.4 pore. Understanding the different trafficking programs of Kv1 channels, and whether they are altered by glycosylation, will highlight the different posttranslational mechanisms available to cells to modify their cell surface ion channel levels and possibly their signaling characteristics.

Received for publication, September 4, 2003 , and in revised form, December 18, 2003.

^* This research was supported by National Institutes of Health Grant NS29633 (to W. B. T.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed. Tel.: 718-817-3688; Fax: 718-817-3645; E-mail: thornhill{at}fordham.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. Mckeown, M. P. Burnham, C. Hodson, and O. T. Jones Identification of an Evolutionarily Conserved Extracellular Threonine Residue Critical for Surface Expression and Its Potential Coupling of Adjacent Voltage-sensing and Gating Domains in Voltage-gated Potassium Channels J. Biol. Chem., October 31, 2008; 283(44): 30421 - 30432. [Abstract] [Full Text] [PDF]

				J.-H. Lee, B.-H. Lee, S.-H. Choi, I.-S. Yoon, M. K. Pyo, T.-J. Shin, W.-S. Choi, Y. Lim, H. Rhim, K. H. Won, et al. Ginsenoside Rg3 Inhibits Human Kv1.4 Channel Currents by Interacting with the Lys531 Residue Mol. Pharmacol., March 1, 2008; 73(3): 619 - 626. [Abstract] [Full Text] [PDF]

				D. Boassa, C. Ambrosi, F. Qiu, G. Dahl, G. Gaietta, and G. Sosinsky Pannexin1 Channels Contain a Glycosylation Site That Targets the Hexamer to the Plasma Membrane J. Biol. Chem., October 26, 2007; 282(43): 31733 - 31743. [Abstract] [Full Text] [PDF]

				D. F. Steele, J. Eldstrom, and D. Fedida Mechanisms of cardiac potassium channel trafficking J. Physiol., July 1, 2007; 582(1): 17 - 26. [Abstract] [Full Text] [PDF]

				K. Mochizuki, T. Kagawa, A. Numari, M. J. Harris, J. Itoh, N. Watanabe, T. Mine, and I. M. Arias Two N-linked glycans are required to maintain the transport activity of the bile salt export pump (ABCB11) in MDCK II cells Am J Physiol Gastrointest Liver Physiol, March 1, 2007; 292(3): G818 - G828. [Abstract] [Full Text] [PDF]

				B. M. Hagen and K. M. Sanders Deglycosylation of the beta1-subunit of the BK channel changes its biophysical properties Am J Physiol Cell Physiol, October 1, 2006; 291(4): C750 - C756. [Abstract] [Full Text] [PDF]

				H. Xu, Y. Fu, W. Tian, and D. M. Cohen Glycosylation of the osmoresponsive transient receptor potential channel TRPV4 on Asn-651 influences membrane trafficking Am J Physiol Renal Physiol, May 1, 2006; 290(5): F1103 - F1109. [Abstract] [Full Text] [PDF]

				J. Napp, F. Monje, W. Stuhmer, and L. A. Pardo Glycosylation of Eag1 (Kv10.1) Potassium Channels: INTRACELLULAR TRAFFICKING AND FUNCTIONAL CONSEQUENCES J. Biol. Chem., August 19, 2005; 280(33): 29506 - 29512. [Abstract] [Full Text] [PDF]

				W. J. Moody and M. M. Bosma Ion Channel Development, Spontaneous Activity, and Activity-Dependent Development in Nerve and Muscle Cells Physiol Rev, July 1, 2005; 85(3): 883 - 941. [Abstract] [Full Text] [PDF]

				A. Schenzer, T. Friedrich, M. Pusch, P. Saftig, T. J. Jentsch, J. Grotzinger, and M. Schwake Molecular Determinants of KCNQ (Kv7) K+ Channel Sensitivity to the Anticonvulsant Retigabine J. Neurosci., May 18, 2005; 25(20): 5051 - 5060. [Abstract] [Full Text] [PDF]