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J. Biol. Chem., Vol. 279, Issue 10, 9146-9155, March 5, 2004

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Effects of Oxygen and Light Intensity on Transcriptome Expression in Rhodobacter sphaeroides 2.4.1

REDOX ACTIVE GENE EXPRESSION PROFILE^*

Jung Hyeob Roh, William E. Smith, and Samuel Kaplan

From the Department of Microbiology and Molecular Genetics, University of Texas, Health Science Center, Houston, Texas 77030

The roles of oxygen and light on the regulation of photosynthesis gene expression in Rhodobacter sphaeroides 2.4.1 have been well studied over the past 50 years. More recently, the effects of oxygen and light on gene regulation have been shown to involve the interacting redox chains present in R. sphaeroides under diverse growth conditions, and many of the redox carriers comprising these chains have been well studied. However, the expression patterns of those genes encoding these redox carriers, under aerobic and anaerobic photosynthetic growth, have been less well studied. Here, we provide a transcriptional analysis of many of the genes comprising the photosynthesis lifestyle, including genes corresponding to many of the known regulatory elements controlling the response of this organism to oxygen and light. The observed patterns of gene expression are evaluated and discussed in light of our knowledge of the physiology of R. sphaeroides under aerobic and photosynthetic growth conditions. Finally, this analysis has enabled to us go beyond the traditional patterns of gene expression associated with the photosynthesis lifestyle and to consider, for the first time, the full complement of genes responding to oxygen, and variations in light intensity when growing photosynthetically. The data provided here should be considered as a first step in enabling one to model electron flow in R. sphaeroides 2.4.1.

Received for publication, October 22, 2003 , and in revised form, November 25, 2003.

^* This work was supported by Department of Energy Grant OBER DE-FG02-01ER63232 and Grant GM15590 from the United States Public Health Service. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains a detailed list of genes/ORFs that comprise the data described in Fig. 1.

To whom correspondence should be addressed: Dept. of Microbiology and Molecular Genetics, University of Texas Health Science Center, 6431 Fannin St., Houston, TX 77030. Tel.: 713-500-5502; Fax: 713-500-5499; E-mail: samuel.kaplan{at}uth.tmc.edu.

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