HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 11, 9765-9776, March 12, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/11/9765    most recent M310877200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bao, X. Articles by Sugahara, K. Search for Related Content PubMed PubMed Citation Articles by Bao, X. Articles by Sugahara, K. Social Bookmarking                      What's this?

Chondroitin Sulfate/Dermatan Sulfate Hybrid Chains from Embryonic Pig Brain, Which Contain a Higher Proportion of L-Iduronic Acid than Those from Adult Pig Brain, Exhibit Neuritogenic and Growth Factor Binding Activities^*

Xingfeng Bao, Shuji Nishimura, Tadahisa Mikami, Shuhei Yamada, Nobuyuki Itoh¶, and Kazuyuki Sugahara||

From the Department of Biochemistry, Kobe Pharmaceutical University, Higashinada-ku, Kobe 658-8558 and the ^¶Department of Genetic Biochemistry, Kyoto University of Graduate School of Pharmaceutical Sciences, Kyoto 606-8501, Japan

We have shown that over-sulfated chondroitin sulfate/dermatan sulfate (CS/DS) chains from various marine organisms exhibit growth factor binding activities and neurite outgrowth-promoting activities in embryonic mouse hippocampal neurons in vitro. In this study we demonstrated that CS/DS hybrid chains purified from embryonic pig brain displayed marked neuritogenic activity and growth factor binding activities toward fibroblast growth factor 2 (FGF2), FGF10, FGF18, pleiotrophin, and midkine, all of which exhibit neuroregulatory activities in the brain. In contrast, the CS/DS preparation from adult pig brain showed considerably less activity to bind these growth factors and no neuritogenic activity. Structural analysis indicated that the average size of the CS/DS chains was similar (40 kDa) between these two preparations, but the disaccharide compositions differed considerably, with a significant proportion of L-iduronic acid (IdoUA)-containing disaccharides (89%) in the CS/DS chains from embryos but not in those from adults (<1%). Interestingly, both neurite outgrowth-promoting activity and growth factor binding activities of the CS/DS chains from embryos were abolished by digestion not only with chondroitinase ABC but also with chondroitinase B, suggesting that the IdoUA-containing motifs are essential for these activities. These findings imply that the temporal expression of CS/DS hybrid structures containing both GlcUA and IdoUA and binding activities toward various growth factors play important roles in neurogenesis in the early stages of the development of the brain.

Received for publication, October 2, 2003 , and in revised form, December 24, 2003.

^* This work was supported in part by the Scientific Research Promotion Fund of the Japan Private School Promotion Foundation Grant-in aid for Exploratory Research 15659021 (to K. S.) and the National Project on Functional Glycoconjugate Research Aimed at Developing New Industry (to K. S.) from the Ministry of Education, Science, Sports, and Culture of Japan. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Recipient of a postdoctoral fellowship from the Japan Society for the Promotion of Science.

^|| To whom correspondence should be addressed: Dept. of Biochemistry, Kobe Pharmaceutical University, 4-19-1, Motoyamakita-machi, Higashinada-ku, Kobe 658-8558, Japan. Tel.: 81-78-441-7570; Fax: 81-78-441-7569; E-mail: k-sugar{at}kobepharma-u.ac.jp.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Ishii and N. Maeda Spatiotemporal expression of chondroitin sulfate sulfotransferases in the postnatal developing mouse cerebellum Glycobiology, August 1, 2008; 18(8): 602 - 614. [Abstract] [Full Text] [PDF]

				D. Shaya, B.-S. Hahn, T. M. Bjerkan, W. S. Kim, N. Y. Park, J.-S. Sim, Y.-S. Kim, and M. Cygler Composite active site of chondroitin lyase ABC accepting both epimers of uronic acid Glycobiology, March 1, 2008; 18(3): 270 - 277. [Abstract] [Full Text] [PDF]

				K. Akita, A. von Holst, Y. Furukawa, T. Mikami, K. Sugahara, and A. Faissner Expression of Multiple Chondroitin/Dermatan Sulfotransferases in the Neurogenic Regions of the Embryonic and Adult Central Nervous System Implies That Complex Chondroitin Sulfates Have a Role in Neural Stem Cell Maintenance Stem Cells, March 1, 2008; 26(3): 798 - 809. [Abstract] [Full Text] [PDF]

				A. J. Hayes, D. Tudor, M. A. Nowell, B. Caterson, and C. E. Hughes Chondroitin Sulfate Sulfation Motifs as Putative Biomarkers for Isolation of Articular Cartilage Progenitor Cells J. Histochem. Cytochem., February 1, 2008; 56(2): 125 - 138. [Abstract] [Full Text] [PDF]

				S. Yamada, H. Morimoto, T. Fujisawa, and K. Sugahara Glycosaminoglycans in Hydra magnipapillata (Hydrozoa, Cnidaria): demonstration of chondroitin in the developing nematocyst, the sting organelle, and structural characterization of glycosaminoglycans Glycobiology, August 1, 2007; 17(8): 886 - 894. [Abstract] [Full Text] [PDF]

				S. S Deepa, S. Yamada, S. Fukui, and K. Sugahara Structural determination of novel sulfated octasaccharides isolated from chondroitin sulfate of shark cartilage and their application for characterizing monoclonal antibody epitopes Glycobiology, June 1, 2007; 17(6): 631 - 645. [Abstract] [Full Text] [PDF]

				F. Li, A. K. Shetty, and K. Sugahara Neuritogenic Activity of Chondroitin/Dermatan Sulfate Hybrid Chains of Embryonic Pig Brain and Their Mimicry from Shark Liver: INVOLVEMENT OF THE PLEIOTROPHIN AND HEPATOCYTE GROWTH FACTOR SIGNALING PATHWAYS J. Biol. Chem., February 2, 2007; 282(5): 2956 - 2966. [Abstract] [Full Text] [PDF]

				C. Mitsunaga, T. Mikami, S. Mizumoto, J. Fukuda, and K. Sugahara Chondroitin Sulfate/Dermatan Sulfate Hybrid Chains in the Development of Cerebellum: SPATIOTEMPORAL REGULATION OF THE EXPRESSION OF CRITICAL DISULFATED DISACCHARIDES BY SPECIFIC SULFOTRANSFERASES J. Biol. Chem., July 14, 2006; 281(28): 18942 - 18952. [Abstract] [Full Text] [PDF]

				M. Ida, T. Shuo, K. Hirano, Y. Tokita, K. Nakanishi, F. Matsui, S. Aono, H. Fujita, Y. Fujiwara, T. Kaji, et al. Identification and Functions of Chondroitin Sulfate in the Milieu of Neural Stem Cells J. Biol. Chem., March 3, 2006; 281(9): 5982 - 5991. [Abstract] [Full Text] [PDF]

				K. Tiedemann, B. Olander, E. Eklund, L. Todorova, M. Bengtsson, M. Maccarana, G. Westergren-Thorsson, and A. Malmstrom Regulation of the chondroitin/dermatan fine structure by transforming growth factor-{beta}1 through effects on polymer-modifying enzymes Glycobiology, December 1, 2005; 15(12): 1277 - 1285. [Abstract] [Full Text] [PDF]

				X. Bao, T. Muramatsu, and K. Sugahara Demonstration of the Pleiotrophin-binding Oligosaccharide Sequences Isolated from Chondroitin Sulfate/Dermatan Sulfate Hybrid Chains of Embryonic Pig Brains J. Biol. Chem., October 21, 2005; 280(42): 35318 - 35328. [Abstract] [Full Text] [PDF]

				X. Bao, M. S. G. Pavao, J. C. dos Santos, and K. Sugahara A Functional Dermatan Sulfate Epitope Containing Iduronate(2-O-sulfate){alpha}1-3GalNAc(6-O-sulfate) Disaccharide in the Mouse Brain: DEMONSTRATION USING A NOVEL MONOCLONAL ANTIBODY RAISED AGAINST DERMATAN SULFATE OF ASCIDIAN ASCIDIA NIGRA J. Biol. Chem., June 17, 2005; 280(24): 23184 - 23193. [Abstract] [Full Text] [PDF]

				Y. Ito, M. Hikino, Y. Yajima, T. Mikami, S. Sirko, A. von Holst, A. Faissner, S. Fukui, and K. Sugahara Structural characterization of the epitopes of the monoclonal antibodies 473HD, CS-56, and MO-225 specific for chondroitin sulfate D-type using the oligosaccharide library Glycobiology, June 1, 2005; 15(6): 593 - 603. [Abstract] [Full Text] [PDF]

				X. Bao, T. Mikami, S. Yamada, A. Faissner, T. Muramatsu, and K. Sugahara Heparin-binding Growth Factor, Pleiotrophin, Mediates Neuritogenic Activity of Embryonic Pig Brain-derived Chondroitin Sulfate/Dermatan Sulfate Hybrid Chains J. Biol. Chem., March 11, 2005; 280(10): 9180 - 9191. [Abstract] [Full Text] [PDF]

				C. D. Nandini, T. Mikami, M. Ohta, N. Itoh, F. Akiyama-Nambu, and K. Sugahara Structural and Functional Characterization of Oversulfated Chondroitin Sulfate/Dermatan Sulfate Hybrid Chains from the Notochord of Hagfish: NEURITOGENIC AND BINDING ACTIVITIES FOR GROWTH FACTORS AND NEUROTROPHIC FACTORS J. Biol. Chem., December 3, 2004; 279(49): 50799 - 50809. [Abstract] [Full Text] [PDF]

				S. S. Deepa, S. Yamada, M. Zako, O. Goldberger, and K. Sugahara Chondroitin Sulfate Chains on Syndecan-1 and Syndecan-4 from Normal Murine Mammary Gland Epithelial Cells Are Structurally and Functionally Distinct and Cooperate with Heparan Sulfate Chains to Bind Growth Factors: A NOVEL FUNCTION TO CONTROL BINDING OF MIDKINE, PLEIOTROPHIN, AND BASIC FIBROBLAST GROWTH FACTOR J. Biol. Chem., September 3, 2004; 279(36): 37368 - 37376. [Abstract] [Full Text] [PDF]