Originally published In Press as doi:10.1074/jbc.C400035200 on January 26, 2004
J. Biol. Chem., Vol. 279, Issue 12, 10829-10832, March 19, 2004
ACCELERATED PUBLICATIONS
Identification of a Role for 
-Catenin in the Establishment of a Bipolar Mitotic Spindle*
Daniel D. Kaplan
,
Thomas E. Meigs¶,
Patrick Kelly||, and
Patrick J. Casey**
From the
Departments of Pharmacology and Cancer Biology and **Biochemistry, Duke University Medical Center, Durham, North Carolina 27710
-Catenin is a multifunctional protein that is known to participate in two well defined cellular processes, cell-cell adhesion and Wnt-stimulated transcriptional activation. Here we report that -catenin participates in a third cellular process, the establishment of a bipolar mitotic spindle. During mitosis, -catenin relocalizes to mitotic spindle poles and to the midbody. Furthermore, biochemical fractionation demonstrates the presence of -catenin in purified centrosome preparations. Reduction of cellular -catenin by RNA interference leads to the failure of centrosomes to fully separate, resulting in a marked increase in the frequency of monoastral mitotic spindles. Our results define a new and important function for -catenin in mitosis and demonstrate that -catenin is involved in vital biological processes beyond cell adhesion and Wnt signaling.
Received for publication, January 20, 2004
* This work was supported by National Institutes of Health Grants CA91159 and CA100869. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The on-line version of this article (available at http://www.jbc.org) contains supplemental Fig. S1.
Howard Hughes Medical Institute Predoctoral Fellow.
¶ Present address: Dept. of Biology, University of North Carolina at Asheville, Asheville, NC 28804.
|| Duke University Medical School Alumni M.D./Ph.D. Scholar.
To whom correspondence should be addressed. Tel.: 919-613-8613; Fax: 919-613-8642; E-mail: casey006{at}mc.duke.edu.
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Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.
