| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 279, Issue 12, 11006-11015, March 19, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the NeuroSearch A/S, Pederstrupvej 93, DK-2750 Ballerup, Denmark
The exact subunit combinations of functional native acid-sensing ion channels (ASICs) have not been established yet, but both homomeric and heteromeric channels are likely to exist. To determine the ability of different subunits to assemble into heteromeric channels, a number of ASIC1a-, ASIC1b-, ASIC2a-, ASIC2b-, and ASIC3-containing homo- and heteromeric channels were studied by whole-cell patch clamp recordings with respect to pH sensitivity, desensitization kinetics, and level of sustained current normalized to peak current. Analyzing and comparing data for these three features demonstrated unique heteromeric channels in a number of co-expression experiments. Formation of heteromeric ASIC1a+2a and ASIC1b+2a channels was foremost supported by the desensitization characteristics that were independent of proton concentration, a feature none of the respective homomeric channels has. Several lines of evidence supported formation of ASIC1a+3, ASIC1b+3, and ASIC2a+3 heteromeric channels. The most compelling was the desensitization characteristics, which, besides being proton-independent, were faster than those of any of the respective homomeric channels. ASIC2b, which homomerically expressed is not activated by protons per se, did not appear to form unique heteromeric combinations with other subunits and in fact appeared to suppress the function of ASIC1b. Co-expression of three subunits such as ASIC1a+2a+3 and ASIC1b+2a+3 resulted in data that could best be explained by coexistence of multiple channel populations within the same cell. This observation seems to be in good agreement with the fact that ASIC-expressing sensory neurons display a variety of acid-evoked currents.
Received for publication, December 10, 2003 , and in revised form, December 26, 2003.
* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 45-44608223; Fax: 45-44608080; E-mail: pka{at}neurosearch.dk.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. R. Light, R. W. Hughen, J. Zhang, J. Rainier, Z. Liu, and J. Lee Dorsal Root Ganglion Neurons Innervating Skeletal Muscle Respond to Physiological Combinations of Protons, ATP, and Lactate Mediated by ASIC, P2X, and TRPV1 J Neurophysiol, September 1, 2008; 100(3): 1184 - 1201. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Baron, N. Voilley, M. Lazdunski, and E. Lingueglia Acid Sensing Ion Channels in Dorsal Spinal Cord Neurons J. Neurosci., February 6, 2008; 28(6): 1498 - 1508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Cho and C. C. Askwith Presynaptic Release Probability Is Increased in Hippocampal Neurons From ASIC1 Knockout Mice J Neurophysiol, February 1, 2008; 99(2): 426 - 441. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. W. Sherwood and C. C. Askwith Endogenous Arginine-Phenylalanine-Amide-related Peptides Alter Steady-state Desensitization of ASIC1a J. Biol. Chem., January 25, 2008; 283(4): 1818 - 1830. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z.-Y. Tan, Y. Lu, C. A. Whiteis, C. J. Benson, M. W. Chapleau, and F. M. Abboud Acid-Sensing Ion Channels Contribute to Transduction of Extracellular Acidosis in Rat Carotid Body Glomus Cells Circ. Res., November 9, 2007; 101(10): 1009 - 1019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Chen, G. Polleichtner, I. Kadurin, and S. Grunder Zebrafish Acid-sensing Ion Channel (ASIC) 4, Characterization of Homo- and Heteromeric Channels, and Identification of Regions Important for Activation by H+ J. Biol. Chem., October 19, 2007; 282(42): 30406 - 30413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Meltzer, N. Kapoor, Y. J. Qadri, S. J. Anderson, C. M. Fuller, and D. J. Benos Heteromeric Assembly of Acid-sensitive Ion Channel and Epithelial Sodium Channel Subunits J. Biol. Chem., August 31, 2007; 282(35): 25548 - 25559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Maher, N.-T. Wu, and H. Ao pH-Insensitive FRET Voltage Dyes J Biomol Screen, August 1, 2007; 12(5): 656 - 667. [Abstract] [PDF]
|
|
|
|
 |
|
 J.-H. Cho and C. C. Askwith Potentiation of acid-sensing ion channels by sulfhydryl compounds Am J Physiol Cell Physiol, June 1, 2007; 292(6): C2161 - C2174. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Cushman, J. Marsh-Haffner, J. P. Adelman, and E. W. McCleskey A Conformation Change in the Extracellular Domain that Accompanies Desensitization of Acid-sensing Ion Channel (ASIC) 3 J. Gen. Physiol., March 26, 2007; 129(4): 345 - 350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Su, Q. Li, K. Shrestha, E. Cormet-Boyaka, L. Chen, P. R. Smith, E. J. Sorscher, D. J. Benos, S. Matalon, and H.-L. Ji Interregulation of Proton-gated Na+ Channel 3 and Cystic Fibrosis Transmembrane Conductance Regulator J. Biol. Chem., December 1, 2006; 281(48): 36960 - 36968. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Poirot, T. Berta, I. Decosterd, and S. Kellenberger Distinct ASIC currents are expressed in rat putative nociceptors and are modulated by nerve injury J. Physiol., October 1, 2006; 576(1): 215 - 234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Mercado, I. A. Lopez, D. Acuna, R. Vega, and E. Soto Acid-Sensing Ionic Channels in the Rat Vestibular Endorgans and Ganglia J Neurophysiol, September 1, 2006; 96(3): 1615 - 1624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yagi, H. N. Wenk, L. A. Naves, and E. W. McCleskey Sustained Currents Through ASIC3 Ion Channels at the Modest pH Changes That Occur During Myocardial Ischemia Circ. Res., September 1, 2006; 99(5): 501 - 509. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. I. Pidoplichko and J. A. Dani Acid-sensitive ionic channels in midbrain dopamine neurons are sensitive to ammonium, which may contribute to hyperammonemia damage PNAS, July 25, 2006; 103(30): 11376 - 11380. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Gu and L.-Y. Lee Characterization of acid signaling in rat vagal pulmonary sensory neurons Am J Physiol Lung Cell Mol Physiol, July 1, 2006; 291(1): L58 - L65. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ettaiche, E. Deval, M. Cougnon, M. Lazdunski, and N. Voilley Silencing Acid-Sensing Ion Channel 1a Alters Cone-Mediated Retinal Function J. Neurosci., May 24, 2006; 26(21): 5800 - 5809. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Jiang, K. K. Rau, R. D. Johnson, and B. Y. Cooper Proton Sensitivity Ca2+ Permeability and Molecular Basis of Acid-Sensing Ion Channels Expressed in Glabrous and Hairy Skin Afferents J Neurophysiol, April 1, 2006; 95(4): 2466 - 2478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Zhang, F. J. Sigworth, and C. M. Canessa Gating of Acid-sensitive Ion Channel-1: Release of Ca2+ Block vs. Allosteric Mechanism J. Gen. Physiol., January 30, 2006; 127(2): 109 - 117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Dang, K. Bielefeldt, and G. F. Gebhart Differential Responses of Bladder Lumbosacral and Thoracolumbar Dorsal Root Ganglion Neurons to Purinergic Agonists, Protons, and Capsaicin J. Neurosci., April 13, 2005; 25(15): 3973 - 3984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sugiura, K. Dang, K. Lamb, K. Bielefeldt, and G. F. Gebhart Acid-Sensing Properties in Rat Gastric Sensory Neurons from Normal and Ulcerated Stomach J. Neurosci., March 9, 2005; 25(10): 2617 - 2627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. P Gitterman, J Wilson, and A. D Randall Functional properties and pharmacological inhibition of ASIC channels in the human SJ-RH30 skeletal muscle cell line J. Physiol., February 1, 2005; 562(3): 759 - 769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Price, R. J. Thompson, J. O. Eshcol, J. A. Wemmie, and C. J. Benson Stomatin Modulates Gating of Acid-sensing Ion Channels J. Biol. Chem., December 17, 2004; 279(51): 53886 - 53891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. G. Jones, R. Slater, H. Cadiou, P. McNaughton, and S. B. McMahon Acid-Induced Pain and Its Modulation in Humans J. Neurosci., December 1, 2004; 24(48): 10974 - 10979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-J. Wu, B. Duan, Y.-D. Mei, J. Gao, J.-G. Chen, M. Zhuo, L. Xu, M. Wu, and T.-L. Xu Characterization of Acid-sensing Ion Channels in Dorsal Horn Neurons of Rat Spinal Cord J. Biol. Chem., October 15, 2004; 279(42): 43716 - 43724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Poirot, M. Vukicevic, A. Boesch, and S. Kellenberger Selective Regulation of Acid-sensing Ion Channel 1 by Serine Proteases J. Biol. Chem., September 10, 2004; 279(37): 38448 - 38457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Askwith, J. A. Wemmie, M. P. Price, T. Rokhlina, and M. J. Welsh Acid-sensing Ion Channel 2 (ASIC2) Modulates ASIC1 H+-activated Currents in Hippocampal Neurons J. Biol. Chem., April 30, 2004; 279(18): 18296 - 18305. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
