HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 12, 11112-11118, March 19, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/12/11112    most recent M312947200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tang, W. Articles by Hemler, M. E. Search for Related Content PubMed PubMed Citation Articles by Tang, W. Articles by Hemler, M. E. Social Bookmarking                      What's this?

Caveolin-1 Regulates Matrix Metalloproteinases-1 Induction and CD147/EMMPRIN Cell Surface Clustering^*

Wei Tang and Martin E. Hemler

From the Dana-Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115

CD147, a regulator of matrix metalloproteinase (MMP) production, showed highly specific association with caveolin-1 on the surface of multiple cell types. CD147-caveolin-1 complex formation was temperature and cholesterol dependent, reminiscent of associations seen within caveolae/lipid rafts. However, the subset of caveolin-1 associated with CD147 appeared exclusively within intermediate density sucrose gradient fractions, rather than in the low density fractions containing the bulk of caveolin-1. Mutagenesis experiments revealed that CD147 Ig domain 2 was required for caveolin-1 association. In contrast to CD147-caveolin-1 complexes, CD147-₃ integrin association was not disrupted upon cholesterol depletion, occurred in high density sucrose fractions, and did not involve CD147 Ig domain 2. Overexpression of caveolin-1 caused a specific decrease in clustering of cell surface CD147, as detected by "cluster specific" mAb M6/13. Conversely, a mutant CD147 deficient in caveolin-1 association showed enhanced spontaneous cell surface clustering (detected by mAb M6/13), and did not show decreased clustering in response to caveolin-1 overexpression. Furthermore, the same CD147 mutant yielded an elevated induction of MMP-1. In conclusion, caveolin-1 associates with CD147, in a complex distinct from CD147-₃ integrin complexes, thereby diminishing both CD147 clustering and CD147-dependent MMP-1-inducing activity.

Received for publication, November 26, 2003 , and in revised form, December 27, 2003.

^* This work was supported by National Institutes of Health Grant CA86712. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dana-Farber Cancer Institute, Rm. D-1430, 44 Binney St., Boston, MA 02115. Tel.: 617-632-3410; Fax: 617-632-2662; E-mail: Martin_Hemler{at}dfci.harvard.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. Sharma, X. H. Yang, and M. E. Hemler DHHC2 Affects Palmitoylation, Stability, and Functions of Tetraspanins CD9 and CD151 Mol. Biol. Cell, August 1, 2008; 19(8): 3415 - 3425. [Abstract] [Full Text] [PDF]

				K. S. Vetrivel, X. Zhang, X. Meckler, H. Cheng, S. Lee, P. Gong, K. O. Lopes, Y. Chen, N. Iwata, K.-J. Yin, et al. Evidence That CD147 Modulation of {beta}-Amyloid (A{beta}) Levels Is Mediated by Extracellular Degradation of Secreted A{beta} J. Biol. Chem., July 11, 2008; 283(28): 19489 - 19498. [Abstract] [Full Text] [PDF]

				X.-L. Yu, T. Hu, J.-M. Du, J.-P. Ding, X.-M. Yang, J. Zhang, B. Yang, X. Shen, Z. Zhang, W.-D. Zhong, et al. Crystal Structure of HAb18G/CD147: IMPLICATIONS FOR IMMUNOGLOBULIN SUPERFAMILY HOMOPHILIC ADHESION J. Biol. Chem., June 27, 2008; 283(26): 18056 - 18065. [Abstract] [Full Text] [PDF]

				N. Odajima, T. Betsuyaku, Y. Nasuhara, and M. Nishimura Loss of Caveolin-1 in Bronchiolization in Lung Fibrosis J. Histochem. Cytochem., September 1, 2007; 55(9): 899 - 909. [Abstract] [Full Text] [PDF]

				J. Tang, H.-w. Zhou, J.-l. Jiang, X.-m. Yang, Y. Li, H.-X. Zhang, Z.-n. Chen, and W.-p. Guo {beta}ig-h3 Is Involved in the HAb18G/CD147-Mediated Metastasis Process in Human Hepatoma Cells Experimental Biology and Medicine, March 1, 2007; 232(3): 344 - 352. [Abstract] [Full Text] [PDF]

				D. T. Browman, M. E. Resek, L. D. Zajchowski, and S. M. Robbins Erlin-1 and erlin-2 are novel members of the prohibitin family of proteins that define lipid-raft-like domains of the ER J. Cell Sci., August 1, 2006; 119(15): 3149 - 3160. [Abstract] [Full Text] [PDF]

				X. H. Yang, O. V. Kovalenko, T. V. Kolesnikova, M. M. Andzelm, E. Rubinstein, J. L. Strominger, and M. E. Hemler Contrasting Effects of EWI Proteins, Integrins, and Protein Palmitoylation on Cell Surface CD9 Organization J. Biol. Chem., May 5, 2006; 281(18): 12976 - 12985. [Abstract] [Full Text] [PDF]

				D. Xu and M. E. Hemler Metabolic Activation-related CD147-CD98 Complex Mol. Cell. Proteomics, August 1, 2005; 4(8): 1061 - 1071. [Abstract] [Full Text] [PDF]

				T. Pushkarsky, V. Yurchenko, C. Vanpouille, B. Brichacek, I. Vaisman, S. Hatakeyama, K. I. Nakayama, B. Sherry, and M. I. Bukrinsky Cell Surface Expression of CD147/EMMPRIN Is Regulated by Cyclophilin 60 J. Biol. Chem., July 29, 2005; 280(30): 27866 - 27871. [Abstract] [Full Text] [PDF]

				A. NAVARRO, B. ANAND-APTE, and M.-O. PARAT A role for caveolae in cell migration FASEB J, December 1, 2004; 18(15): 1801 - 1811. [Abstract] [Full Text] [PDF]

				W. Tang, S. B. Chang, and M. E. Hemler Links between CD147 Function, Glycosylation, and Caveolin-1 Mol. Biol. Cell, September 1, 2004; 15(9): 4043 - 4050. [Abstract] [Full Text] [PDF]