HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 12, 11875-11881, March 19, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/12/11875    most recent M310717200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Katagiri, K. Articles by Kinashi, T. Search for Related Content PubMed PubMed Citation Articles by Katagiri, K. Articles by Kinashi, T. Social Bookmarking                      What's this?

Rap1-mediated Lymphocyte Function-associated Antigen-1 Activation by the T Cell Antigen Receptor Is Dependent on Phospholipase C-1^*

Koko Katagiri, Mika Shimonaka, and Tatsuo Kinashi¶

From the Bayer-chair Department of Molecular Immunology and Allergy and Department of Dermatology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan

The small GTPase, Rap1, is a potent activator of leukocyte integrins and enhances the adhesive activity of lymphocyte function-associated antigen-1 (LFA-1) when stimulated by the T cell receptor (TCR) or chemokines. However, the mechanism by which Rap1 is activated remains unclear. Here, we demonstrate that phospholipase C (PLC)-1 plays a critical role in the signaling pathway leading to Rap1 activation triggered by the TCR. In Jurkat T cells, TCR cross-linking triggered persistent Rap1 activation, and SDF-1 (CXCL12) activated Rap1 transiently. A phospholipase C inhibitor, U73122, abrogated Rap1 activation triggered by both the TCR and SDF-1 (CXCL12). PLC-1-deficient Jurkat T cells showed a marked reduction of TCR-triggered Rap1 activation and adhesion to intercellular adhesion molecule-1 (ICAM-1) mediated by LFA-1. In contrast, SDF-1-triggered Rap1 activation and adhesion were not affected in these cells. Transfection of these cells with an expression plasmid encoding PLC-1 restored Rap1 activation by the TCR and the ability to adhere to ICAM-1, accompanied by polarized LFA-1 surface clustering colocalized with regulator of adhesion and polarization enriched in lymphoid tissues (RAPL). Furthermore, when expressed in Jurkat cells, CalDAG-GEFI, a calcium and diacylglycerol-responsive Rap1 exchange factor, associated with Rap1, and resulted in enhanced Rap1 activation and adhesion triggered by the TCR. Our results demonstrate that TCR activation of Rap1 depends on PLC-1. This activity is likely to be mediated by CalDAG-GEFI, which is required to activate LFA-1.

Received for publication, September 29, 2003 , and in revised form, December 28, 2003.

^* This work was supported in part by a grant-in-aid from the Ministry of Education, Science, Sports, and Culture of Japan and by the Cell Science Research Foundation. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence and reprint requests should be addressed: Bayer-chair Dept. of Molecular Immunology and Allergy, Graduate School of Medicine, Kyoto University, Yoshida-konoe, Sakyo-ku, Kyoto 606-8501, Japan. Tel.: 81-75-771-8159; Fax: 81-75-771-8184; E-mail: tkinashi{at}mfour.med.kyoto-u.ac.jp.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				E. Arana, N. E. Harwood, and F. D. Batista Regulation of integrin activation through the B-cell receptor J. Cell Sci., July 15, 2008; 121(14): 2279 - 2286. [Abstract] [Full Text] [PDF]

				Y. Chen, M. Yu, A. Podd, R. Wen, M. Chrzanowska-Wodnicka, G. C. White, and D. Wang A critical role of Rap1b in B-cell trafficking and marginal zone B-cell development Blood, May 1, 2008; 111(9): 4627 - 4636. [Abstract] [Full Text] [PDF]

				L. H. Ulfman, V. M. Kamp, C. W. van Aalst, L. P. Verhagen, M. E. Sanders, K. A. Reedquist, M. Buitenhuis, and L. Koenderman Homeostatic Intracellular-Free Ca2+ Is Permissive for Rap1-Mediated Constitutive Activation of {alpha}4 Integrins on Eosinophils J. Immunol., April 15, 2008; 180(8): 5512 - 5519. [Abstract] [Full Text] [PDF]

				C. D. Sharp, M. Huang, J. Glawe, D. R. Patrick, S. Pardue, S. C. Barlow, and C. G. Kevil Stromal Cell Derived Factor-1/CXCL12 Stimulates Chemorepulsion of NOD/LtJ T-Cell Adhesion to Islet Microvascular Endothelium Diabetes, January 1, 2008; 57(1): 102 - 112. [Abstract] [Full Text] [PDF]

				H. Ghandour, X. Cullere, A. Alvarez, F. W. Luscinskas, and T. N. Mayadas Essential role for Rap1 GTPase and its guanine exchange factor CalDAG-GEFI in LFA-1 but not VLA-4 integrin mediated human T-cell adhesion Blood, November 15, 2007; 110(10): 3682 - 3690. [Abstract] [Full Text] [PDF]

				J. C. Nolz, R. B. Medeiros, J. S. Mitchell, P. Zhu, B. D. Freedman, Y. Shimizu, and D. D. Billadeau WAVE2 Regulates High-Affinity Integrin Binding by Recruiting Vinculin and Talin to the Immunological Synapse Mol. Cell. Biol., September 1, 2007; 27(17): 5986 - 6000. [Abstract] [Full Text] [PDF]

				R. Pasvolsky, S. W. Feigelson, S. S. Kilic, A. J. Simon, G. Tal-Lapidot, V. Grabovsky, J. R. Crittenden, N. Amariglio, M. Safran, A. M. Graybiel, et al. A LAD-III syndrome is associated with defective expression of the Rap-1 activator CalDAG-GEFI in lymphocytes, neutrophils, and platelets J. Exp. Med., July 9, 2007; 204(7): 1571 - 1582. [Abstract] [Full Text] [PDF]

				T. W. Kuijpers, R. v. Bruggen, N. Kamerbeek, A. T. J. Tool, G. Hicsonmez, A. Gurgey, A. Karow, A. J. Verhoeven, K. Seeger, O. Sanal, et al. Natural history and early diagnosis of LAD-1/variant syndrome Blood, April 15, 2007; 109(8): 3529 - 3537. [Abstract] [Full Text] [PDF]

				M. S. Osman, D. N. Burshtyn, and K. P. Kane Activating Ly-49 Receptors Regulate LFA-1-Mediated Adhesion by NK Cells J. Immunol., February 1, 2007; 178(3): 1261 - 1267. [Abstract] [Full Text] [PDF]

				W. T. N. Simonson, S. J. Franco, and A. Huttenlocher Talin1 Regulates TCR-Mediated LFA-1 Function J. Immunol., December 1, 2006; 177(11): 7707 - 7714. [Abstract] [Full Text] [PDF]

				S. Kliche, D. Breitling, M. Togni, R. Pusch, K. Heuer, X. Wang, C. Freund, A. Kasirer-Friede, G. Menasche, G. A. Koretzky, et al. The ADAP/SKAP55 Signaling Module Regulates T-Cell Receptor-Mediated Integrin Activation through Plasma Membrane Targeting of Rap1. Mol. Cell. Biol., October 1, 2006; 26(19): 7130 - 7144. [Abstract] [Full Text] [PDF]

				L. D. Finkelstein, Y. Shimizu, and P. L. Schwartzberg Tec Kinases Regulate TCR-Mediated Recruitment of Signaling Molecules and Integrin-Dependent Cell Adhesion J. Immunol., November 1, 2005; 175(9): 5923 - 5930. [Abstract] [Full Text] [PDF]

				L. H. Ulfman, J. Alblas, C. W. van Aalst, J. J. Zwaginga, and L. Koenderman Differences in Potency of CXC Chemokine Ligand 8-, CC Chemokine Ligand 11-, and C5a-Induced Modulation of Integrin Function on Human Eosinophils J. Immunol., November 1, 2005; 175(9): 6092 - 6099. [Abstract] [Full Text] [PDF]

				W. Ise, K. Nakamura, N. Shimizu, H. Goto, K. Fujimoto, S. Kaminogawa, and S. Hachimura Orally Tolerized T Cells Can Form Conjugates with APCs but Are Defective in Immunological Synapse Formation J. Immunol., July 15, 2005; 175(2): 829 - 838. [Abstract] [Full Text] [PDF]

				M. R. Tardif and M. J. Tremblay Regulation of LFA-1 Activity through Cytoskeleton Remodeling and Signaling Components Modulates the Efficiency of HIV Type-1 Entry in Activated CD4+ T Lymphocytes J. Immunol., July 15, 2005; 175(2): 926 - 935. [Abstract] [Full Text] [PDF]

				S. Vielkind, M. Gallagher-Gambarelli, M. Gomez, H. J. Hinton, and D. A. Cantrell Integrin Regulation by RhoA in Thymocytes J. Immunol., July 1, 2005; 175(1): 350 - 357. [Abstract] [Full Text] [PDF]

				D. Garcia-Bernal, N. Wright, E. Sotillo-Mallo, C. Nombela-Arrieta, J. V. Stein, X. R. Bustelo, and J. Teixido Vav1 and Rac Control Chemokine-promoted T Lymphocyte Adhesion Mediated by the Integrin {alpha}4{beta}1 Mol. Biol. Cell, July 1, 2005; 16(7): 3223 - 3235. [Abstract] [Full Text] [PDF]

				M. Semmrich, A. Smith, C. Feterowski, S. Beer, B. Engelhardt, D. H. Busch, B. Bartsch, M. Laschinger, N. Hogg, K. Pfeffer, et al. Importance of integrin LFA-1 deactivation for the generation of immune responses J. Exp. Med., June 20, 2005; 201(12): 1987 - 1998. [Abstract] [Full Text] [PDF]

				S. K. Shaw, S. Ma, M. B. Kim, R. M. Rao, C. U. Hartman, R. M. Froio, L. Yang, T. Jones, Y. Liu, A. Nusrat, et al. Coordinated Redistribution of Leukocyte LFA-1 and Endothelial Cell ICAM-1 Accompany Neutrophil Transmigration J. Exp. Med., December 20, 2004; 200(12): 1571 - 1580. [Abstract] [Full Text] [PDF]