HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 12, 11917-11925, March 19, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/12/11917    most recent M310076200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Runko, A. P. Articles by Sagerström, C. G. Search for Related Content PubMed PubMed Citation Articles by Runko, A. P. Articles by Sagerström, C. G. Social Bookmarking                      What's this?

Isolation of nlz2 and Characterization of Essential Domains in Nlz Family Proteins^*

Alexander P. Runko and Charles G. Sagerström

From the Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605

In this study, we first cloned nlz2, a second zebrafish member of the nlz-related zinc-finger gene family. nlz2 was expressed together with nlz1 in a broad posterior domain during gastrula stages as well as at the midbrain-hindbrain boundary and in the hindbrain caudal to rhombomere 4 during segmentation. nlz2 was also expressed in regions distinct from nlz1, notably in the forebrain, midbrain, and trunk. Misexpression of nlz2 in zebrafish embryos disrupted gene expression in the rostral hindbrain, similar to the effect of misexpressing nlz1. We next compared the nlz1 and nlz2 sequences to identify and characterize domains conserved within this family. We found a C-terminal domain required for nuclear localization and two conserved domains (the Sp motif and a putative C₂H₂ zinc finger) required for nlz1 function. We also demonstrate that Nlz1 self-associated via its C terminus, interacted with Nlz2, and bound to histone deacetylases. Last, we found two forms of Nlz1 generated from alternative translation initiation sites in vivo. These forms have distinct activities, apparently depending on the function of the N-terminal Sp motif. Our data demonstrate that nlz2 functions similarly to nlz1 and define conserved domains essential for nuclear localization, self-association, and corepressor binding in this novel family of zinc-finger genes.

Received for publication, September 10, 2003 , and in revised form, December 19, 2003.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EBI Data Bank with accession number(s) AY371081.

^* This work was supported by National Institutes of Health Grants NS38183 and HD39156 (to C. G. S.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dept. of Biochemistry and Molecular Pharmacology, LRB 822, 364 Plantation St., Worcester, MA 01605. Tel.: 508-856-8006; Fax: 508-856-8007; E-mail: charles.sagerstrom{at}umassmed.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. D. Brown, S. Dutta, K. Bharti, R. F. Bonner, P. J. Munson, I. B. Dawid, A. L. Akhtar, I. F. Onojafe, R. P. Alur, J. M. Gross, et al. Expression profiling during ocular development identifies 2 Nlz genes with a critical role in optic fissure closure PNAS, February 3, 2009; 106(5): 1462 - 1467. [Abstract] [Full Text] [PDF]

				S.-J. Ji, G. Periz, and S. Sockanathan Nolz1 is induced by retinoid signals and controls motoneuron subtype identity through distinct repressor activities Development, January 15, 2009; 136(2): 231 - 240. [Abstract] [Full Text] [PDF]