HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 13, 12763-12768, March 26, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/13/12763    most recent M311160200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lee, Y.-L. Articles by Yeung, W. S. B. Search for Related Content PubMed PubMed Citation Articles by Lee, Y.-L. Articles by Yeung, W. S. B. Social Bookmarking                      What's this?

The Embryotrophic Activity of Oviductal Cell-derived Complement C3b and iC3b, a Novel Function of Complement Protein in Reproduction^*

Yin-Lau Lee, Kai-Fai Lee, Jia-Sen Xu, Qing-Yu He, Jen-Fu Chiu¶, Will M. Lee||, John M. Luk**, and William S. B. Yeung

From the Department of Obstetrics and Gynaecology, Department of Chemistry, ^¶Institute of Molecular Biology, ^||Department of Zoology and ^**Department of Surgery, University of Hong Kong, Pokfulam Road, Hong Kong SAR, China

The oviduct-derived embryotrophic factor, ETF-3, enhances the development of trophectoderm and the hatching process of treated embryos. Monoclonal anti-ETF-3 antibody that abolishes the embryotrophic activity of ETF-3 recognized a 115-kDa protein from the conditioned medium of immortalized human oviductal cells. Mass spectrometry analysis showed that the protein was complement C3. Western blot analysis using an antibody against C3 confirmed the cross-reactivities between anti-C3 antibody with ETF-3 and anti-ETF-3 antibody with C3 and its derivatives, C3b and iC3b. Both derivatives, but not C3, were embryotrophic. iC3b was most efficient in enhancing the development of blastocysts with larger size and higher hatching rate, consistent with the previous reported embryotrophic activity of ETF-3. Embryos treated with iC3b contained iC3b immunoreactivity. The oviductal epithelium produced C3 as evidenced by the presence of C3 immunoreactivity and mRNA in the human oviduct and cultured oviductal cells. Cyclical changes in the expression of C3 immunoreactivity and mRNA were also found in the mouse oviduct with the highest expression at the estrus stage. Molecules involving in the conversion of C3b to iC3b and binding of iC3b were present in the human oviduct (factor I) and mouse preimplantation embryo (Crry and CR3), respectively. In conclusion, the present data showed that the oviduct produced C3/C3b, which was converted to iC3b to stimulate embryo development.

Received for publication, October 10, 2003 , and in revised form, December 16, 2003.

^* This work was supported by Research Grant Council, Hong Kong, Grant HKU7319/01M (to W. S. B. Y.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: 852-2855-3405; Fax: 852-2855-0947; E-mail: wsbyeung{at}hkucc.hku.hk.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. W.Y. Cheong, Y.-L. Lee, W.-M. Liu, W. S.B. Yeung, and K.-F. Lee Oviductal Microsomal Epoxide Hydrolase (EPHX1) Reduces Reactive Oxygen Species (ROS) Level and Enhances Preimplantation Mouse Embryo Development Biol Reprod, July 1, 2009; 81(1): 126 - 132. [Abstract] [Full Text] [PDF]

				R. E Lloyd, R. Romar, C. Matas, A. Gutierrez-Adan, W. V Holt, and P. Coy Effects of oviductal fluid on the development, quality, and gene expression of porcine blastocysts produced in vitro Reproduction, April 1, 2009; 137(4): 679 - 687. [Abstract] [Full Text] [PDF]

				P. C.N. Chiu, B. S.T. Wong, C.L. Lee, R. T.K. Pang, K.-F. Lee, S.B. Sumitro, S.K. Gupta, and W. S.B. Yeung Native human zona pellucida glycoproteins: purification and binding properties Hum. Reprod., June 1, 2008; 23(6): 1385 - 1393. [Abstract] [Full Text] [PDF]

				P.-K. Tse, Y.-L. Lee, W.-N. Chow, J. M. C. Luk, K.-F. Lee, and W. S. B. Yeung Preimplantation Embryos Cooperate with Oviductal Cells to Produce Embryotrophic Inactivated Complement-3b Endocrinology, March 1, 2008; 149(3): 1268 - 1276. [Abstract] [Full Text] [PDF]

				P. C. N. Chiu, M.-K. Chung, R. Koistinen, H. Koistinen, M. Seppala, P.-C. Ho, E. H. Y. Ng, K.-F. Lee, and W. S. B. Yeung Cumulus Oophorus-associated Glycodelin-C Displaces Sperm-bound Glycodelin-A and -F and Stimulates Spermatozoa-Zona Pellucida Binding J. Biol. Chem., February 23, 2007; 282(8): 5378 - 5388. [Abstract] [Full Text] [PDF]

				P. C. N. Chiu, M.-K. Chung, R. Koistinen, H. Koistinen, M. Seppala, P.-C. Ho, E. H. Y. Ng, K.-F. Lee, and W. S. B. Yeung Glycodelin-A interacts with fucosyltransferase on human sperm plasma membrane to inhibit spermatozoa-zona pellucida binding J. Cell Sci., January 1, 2007; 120(1): 33 - 44. [Abstract] [Full Text] [PDF]

				K.-F. Lee, J.-S. Xu, Y.-L. Lee, and W. S. B. Yeung Demilune Cell and Parotid Protein from Murine Oviductal Epithelium Stimulates Preimplantation Embryo Development Endocrinology, January 1, 2006; 147(1): 79 - 87. [Abstract] [Full Text] [PDF]

				J.S. Xu, Y.L. Lee, K.F. Lee, K.L. Kwok, W.M. Lee, J.M. Luk, and W.S.B. Yeung Embryotrophic factor-3 from human oviductal cells enhances proliferation, suppresses apoptosis and stimulates the expression of the {beta}1 subunit of sodium-potassium ATPase in mouse embryos Hum. Reprod., December 1, 2004; 19(12): 2919 - 2926. [Abstract] [Full Text] [PDF]