HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 14, 13584-13592, April 2, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/14/13584    most recent M311361200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gazo, B. M. Articles by Browning, K. S. Search for Related Content PubMed PubMed Citation Articles by Gazo, B. M. Articles by Browning, K. S. Social Bookmarking                      What's this?

A Novel Interaction of Cap-binding Protein Complexes Eukaryotic Initiation Factor (eIF) 4F and eIF(iso)4F with a Region in the 3'-Untranslated Region of Satellite Tobacco Necrosis Virus^*

Brandy M. Gazo, Patricia Murphy, Jennifer R. Gatchel, and Karen S. Browning

From the Department of Chemistry and Biochemistry and the Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712

Satellite tobacco necrosis virus (STNV) RNA is naturally uncapped at its 5' end and lacks polyadenylation at its 3' end. Despite lacking these two hallmarks of eukaryotic mRNAs, STNV-1 RNA is translated very efficiently. A 130-nucleotide translational enhancer (TED), located 3' to the termination codon, is necessary for efficient cap-independent translation of STNV-1 RNA. The STNV-1 TED RNA fragment binds to the eukaryotic cap-binding complexes, initiation factor (eIF) 4F and eIF(iso)4F, as measured by nitrocellulose binding and fluorescence titration. STNV-1 TED is a potent inhibitor of in vitro translation when added in trans. This inhibition is reversed by the addition of eIF4F or eIF(iso)4F, and the subunits of eIF4F and eIF(iso)4F cross-link to STNV-1 TED, providing additional evidence that these factors interact directly with STNV-1 TED. Deletion mutagenesis of the STNV-1 TED indicates that a minimal region of 100 nucleotides is necessary to promote cap-independent translation primarily through interaction with the cap binding subunits (eIF4E or eIF(iso)4E) of eIF4F or eIF(iso)4F.

Received for publication, October 15, 2003 , and in revised form, January 8, 2004.

^* This work was supported by Welch Foundation Grant F-1339 and National Science Foundation Grant MCB0214996 (to K. S. B.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dept. of Chemistry and Biochemistry, University of Texas at Austin, 1 University Station A5300, Austin, TX 78712. Tel.: 512-471-4562; Fax: 512-471-8696; E-mail: kbrowning{at}mail.utexas.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Z. Wang, K. Treder, and W. A. Miller Structure of a Viral Cap-independent Translation Element That Functions via High Affinity Binding to the eIF4E Subunit of eIF4F J. Biol. Chem., May 22, 2009; 284(21): 14189 - 14202. [Abstract] [Full Text] [PDF]

				K. Treder, E. L. Pettit Kneller, E. M. Allen, Z. Wang, K. S. Browning, and W. A. Miller The 3' cap-independent translation element of Barley yellow dwarf virus binds eIF4F via the eIF4G subunit to initiate translation RNA, January 1, 2008; 14(1): 134 - 147. [Abstract] [Full Text] [PDF]

				A. M. Rakotondrafara, C. Polacek, E. Harris, and W. A. Miller Oscillating kissing stem-loop interactions mediate 5' scanning-dependent translation by a viral 3'-cap-independent translation element RNA, October 1, 2006; 12(10): 1893 - 1906. [Abstract] [Full Text] [PDF]

				M. R. Fabian and K. A. White Analysis of a 3'-translation enhancer in a tombusvirus: A dynamic model for RNA-RNA interactions of mRNA termini RNA, July 1, 2006; 12(7): 1304 - 1314. [Abstract] [Full Text] [PDF]

				D. Edgil, C. Polacek, and E. Harris Dengue Virus Utilizes a Novel Strategy for Translation Initiation When Cap-Dependent Translation Is Inhibited J. Virol., March 15, 2006; 80(6): 2976 - 2986. [Abstract] [Full Text] [PDF]

				I. M. Krab, C. Caldwell, D. R. Gallie, and J. F. Bol Coat protein enhances translational efficiency of Alfalfa mosaic virus RNAs and interacts with the eIF4G component of initiation factor eIF4F J. Gen. Virol., June 1, 2005; 86(6): 1841 - 1849. [Abstract] [Full Text] [PDF]

				M. R. Fabian and K. A. White 5'-3' RNA-RNA Interaction Facilitates Cap- and Poly(A) Tail-independent Translation of Tomato Bushy Stunt Virus mRNA: A POTENTIAL COMMON MECHANISM FOR TOMBUSVIRIDAE J. Biol. Chem., July 9, 2004; 279(28): 28862 - 28872. [Abstract] [Full Text] [PDF]