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J. Biol. Chem., Vol. 279, Issue 14, 13721-13728, April 2, 2004

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Preferential Translation of Internal Ribosome Entry Site-containing mRNAs during the Mitotic Cycle in Mammalian Cells^*

Xiaoli Qin and Peter Sarnow

From the Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305

A cell synchronization protocol was established in which global and individual mRNA translational efficiencies could be examined. While global translational efficiency was reduced in mitotic cells, 3% of mRNAs remained predominantly associated with large polysomes during mitosis, as determined by cDNA microarray analyses. The 5'-non-coding regions of six mRNAs were shown to contain internal ribosome entry sites (IRES). However, not all known mRNAs that contain IRES elements were actively translated during mitosis, arguing that specific IRES sequences are differentially regulated during mitosis.

Received for publication, November 25, 2003 , and in revised form, January 21, 2004.

^* This work was supported by Grant GM55979 from the National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Present address: Dept. of Molecular & Cell Biology, University of California, Berkeley, CA 94720.

To whom correspondence should be addressed: Dept. of Microbiology and Immunology, Stanford University School of Medicine, Fairchild Science Bldg., Stanford, CA 94305. Tel.: 650-498-7076; Fax: 650-498-7147; E-mail: psarnow{at}stanford.edu.

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