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J. Biol. Chem., Vol. 279, Issue 15, 15204-15213, April 9, 2004

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Ca²⁺-induced Rolling of Tropomyosin in Muscle Thin Filaments

THE - AND -BAND HYPOTHESIS REVISITED^*

Luis M. F. Holthauzen, Fernando Corrêa, and Chuck S. Farah

From the Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, CEP 05508-900, São Paulo, SP, Brazil

Tropomyosin is a filamentous coiled-coil protein directly involved in the regulation of the actomyosin interaction responsible for muscle contraction: it transmits the local calcium-induced conformational change in troponin to the helical array of myosin-binding sites on the surface of the actin filament. McLachlan and Stewart (McLachlan, A. D., and Stewart, M. (1976) J. Mol. Biol. 103, 271–298) proposed that the tropomyosin coiled-coil structure can be divided into 14 alternating 19- to 20-residue "- and -bands," which could act as alternate 7-fold sets of sites for specific binding to actin in the different conformational states of the regulated thin filament. Here we present the first direct experimental evidence in support of the - and -band hypothesis: we analyze the acrylamide quenching of the fluorescence of mutant tropomyosins containing 5-hydroxytryptophan residues at different positions along the coiled-coil structure under a variety of conditions (alone, complexed with actin, and complexed with actin and troponin with or without Ca²⁺). We show that fluorescent probes placed in the -bands become less solvent-exposed in the absence of calcium, whereas those in the -bands become less solvent-exposed in the presence of calcium. A model in which the tropomyosin coiled-coil rolls across the actin surface in response to Ca²⁺-binding to troponin most easily explains these observations.

Received for publication, August 12, 2003 , and in revised form, January 12, 2004.

^* This work was supported by grants from Fundação de Amparo à Pesquisa do Estado de São Paulo. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: 55-11-3091-3312; Fax: 55-11-3815-5579; E-mail: chsfarah{at}iq.usp.br.

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