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J. Biol. Chem., Vol. 279, Issue 16, 15728-15733, April 16, 2004

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Aep3p Stabilizes the Mitochondrial Bicistronic mRNA Encoding Subunits 6 and 8 of the H⁺-translocating ATP Synthase of Saccharomyces cerevisiae^*

Timothy P. Ellis, Kevin G. Helfenbein, Alexander Tzagoloff, and Carol L. Dieckmann¶

From the Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721 and Department of Biological Sciences, Columbia University, New York, New York 10027

Subunits 6 and 8 of the mitochondrial ATPase in Saccharomyces cerevisiae are encoded by the mitochondrial genome and translated from bicistronic mRNAs containing both reading frames. The stability of the two major species of ATP8/6 mRNA, which differ in the length of the 5'-untranslated region, depends on the expression of several nuclear-encoded factors. In the present study, the product of the gene designated AEP3 (open reading frame YPL005W) is shown to be required for stabilization and/or processing of both ATP8/6 mRNA species. In an aep3-disruptant strain, the shorter ATP8/6 mRNA was undetectable, and the level of the longer mRNA was reduced to 35% that of wild type. Localization of a hemagglutinin-tagged version of Aep3p showed that the protein is an extrinsic constituent of the mitochondrial inner membrane facing the matrix.

Received for publication, December 24, 2003 , and in revised form, January 21, 2004.

^* This research was supported by NIH Research Grants GM34893 (to C. L. D.) and HL2274 (to A. T.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed: Dept. of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, AZ 85721. Tel.: 520-621-3569; Fax: 520-621-3709; E-mail: dieckman{at}u.arizona.edu.

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