HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 16, 15850-15859, April 16, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/16/15850    most recent M313613200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kreikemeyer, B. Articles by Podbielski, A. Search for Related Content PubMed PubMed Citation Articles by Kreikemeyer, B. Articles by Podbielski, A. Social Bookmarking                      What's this?

Streptococcus pyogenes Fibronectin-binding Protein F2

EXPRESSION PROFILE, BINDING CHARACTERISTICS, AND IMPACT ON EUKARYOTIC CELL INTERACTIONS^*

Bernd Kreikemeyer, Sonja Oehmcke, Masanobu Nakata, Raimund Hoffrogge¶, and Andreas Podbielski||

From the Department of Medical Microbiology and Hospital Hygiene, Hospital of the Rostock University, Schillingallee 70, 18057 Rostock and the ^¶Proteome Center Rostock, Medical Faculty, University of Rostock, Joachim-Jungius-Strasse 9, 18059 Rostock, Germany

Some Streptococcus pyogenes (group A streptococci, GAS) strains have previously been shown to express the fibronectin-binding protein F2 instead of the functionally related but structurally dissimilar protein F1/SfbI. In this study, recombinant N-terminal and C-terminal portions and the two fibronectin-binding domains of protein F2 were used to assess affinity parameters of the interaction with fibronectin and its N-terminal 70-, 30-, and 45-kDa fragments. The association and dissociation equilibrium constants for both binding domains were in the nanomolar range, although the repeat domain of protein F2 exceeded the affinity of the unique domain by up to one order magnitude. Both domains primarily interacted with the 30-kDa fibronectin fragment. Using a prtF2 gene isogenic mutant of a serotype M49 GAS strain that does not harbor the protein F1/SfbI gene, the attachment values of whole bacteria to immobilized fibronectin and to HEp-2 epithelial cells were found to be 6- and 2-fold decreased, respectively. Reduction of prtF2 mutant internalization rates for eukaryotic cells exceeded the reduction of attachment rates, indicating an independent contribution of protein F2 to both processes. The prtF2 transcription and protein F2 expression profiles documented maximum expression at the transition to the stationary phase especially under aerobic growth condition. The protein F2 function as the major fibronectin-binding adhesin in a subset of GAS strains, its expression pattern, and highly specific interaction with fibronectin would be consistent with a status as an indispensable virulence factor for both earlier and later pathogenetic stages of GAS superficial infections.

Received for publication, December 12, 2003 , and in revised form, January 21, 2004.

^* This work was supported by Deutsche Forschungsgemeinschaft Grants Po391/11-1 and Kr 1765/2-1. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Both authors contributed equally to this work.

^|| To whom correspondence should be addressed. Tel.: 49-381-494-5900; Fax: 49-81-494-5902; E-mail: andreas.podbielski{at}med.unirostock.de.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Nakata, T. Koller, K. Moritz, D. Ribardo, L. Jonas, K. S. McIver, T. Sumitomo, Y. Terao, S. Kawabata, A. Podbielski, et al. Mode of Expression and Functional Characterization of FCT-3 Pilus Region-Encoded Proteins in Streptococcus pyogenes Serotype M49 Infect. Immun., January 1, 2009; 77(1): 32 - 44. [Abstract] [Full Text] [PDF]

				M. Esgleas, Y. Li, M. A. Hancock, J. Harel, J. D. Dubreuil, and M. Gottschalk Isolation and characterization of {alpha}-enolase, a novel fibronectin-binding protein from Streptococcus suis Microbiology, September 1, 2008; 154(9): 2668 - 2679. [Abstract] [Full Text] [PDF]

				C. M. Gillen, H. S. Courtney, K. Schulze, M. Rohde, M. R. Wilson, A. M. Timmer, C. A. Guzman, V. Nizet, G. S. Chhatwal, and M. J. Walker Opacity Factor Activity and Epithelial Cell Binding by the Serum Opacity Factor Protein of Streptococcus pyogenes Are Functionally Discrete J. Biol. Chem., March 7, 2008; 283(10): 6359 - 6366. [Abstract] [Full Text] [PDF]

				B. Kreikemeyer, M. Nakata, T. Koller, H. Hildisch, V. Kourakos, K. Standar, S. Kawabata, M. O. Glocker, and A. Podbielski The Streptococcus pyogenes Serotype M49 Nra-Ralp3 Transcriptional Regulatory Network and Its Control of Virulence Factor Expression from the Novel eno ralp3 epf sagA Pathogenicity Region Infect. Immun., December 1, 2007; 75(12): 5698 - 5710. [Abstract] [Full Text] [PDF]

				L. Baldassarri, R. Creti, M. Imperi, S. Recchia, M. Pataracchia, and G. Orefici Detection of Genes Encoding Internalization-Associated Proteins in Streptococcus pyogenes Isolates from Patients with Invasive Diseases and Asymptomatic Carriers J. Clin. Microbiol., April 1, 2007; 45(4): 1284 - 1287. [Abstract] [Full Text] [PDF]

				Z. Kratovac, A. Manoharan, F. Luo, S. Lizano, and D. E. Bessen Population Genetics and Linkage Analysis of Loci within the FCT Region of Streptococcus pyogenes J. Bacteriol., February 15, 2007; 189(4): 1299 - 1310. [Abstract] [Full Text] [PDF]

				A. Podbielski Flexible Architecture of the Streptococcus pyogenes FCT Genome Region: Finally the Clue for Understanding Purulent Skin Diseases and Long-Term Persistence?{triangledown} J. Bacteriol., February 15, 2007; 189(4): 1181 - 1184. [Full Text] [PDF]

				C. G. Baums, U. Kaim, M. Fulde, G. Ramachandran, R. Goethe, and P. Valentin-Weigand Identification of a Novel Virulence Determinant with Serum Opacification Activity in Streptococcus suis. Infect. Immun., November 1, 2006; 74(11): 6154 - 6162. [Abstract] [Full Text] [PDF]

				G. S. Tamura, J. R. Hull, M. D. Oberg, and D. G. Castner High-Affinity Interaction between Fibronectin and the Group B Streptococcal C5a Peptidase Is Unaffected by a Naturally Occurring Four-Amino-Acid Deletion That Eliminates Peptidase Activity. Infect. Immun., October 1, 2006; 74(10): 5739 - 5746. [Abstract] [Full Text] [PDF]

				K. Ouhara, H. Komatsuzawa, H. Shiba, Y. Uchida, T. Kawai, K. Sayama, K. Hashimoto, M. A. Taubman, H. Kurihara, and M. Sugai Actinobacillus actinomycetemcomitans Outer Membrane Protein 100 Triggers Innate Immunity and Production of {beta}-Defensin and the 18-Kilodalton Cationic Antimicrobial Protein through the Fibronectin-Integrin Pathway in Human Gingival Epithelial Cells Infect. Immun., September 1, 2006; 74(9): 5211 - 5220. [Abstract] [Full Text] [PDF]

				C. Lembke, A. Podbielski, C. Hidalgo-Grass, L. Jonas, E. Hanski, and B. Kreikemeyer Characterization of Biofilm Formation by Clinically Relevant Serotypes of Group A Streptococci Appl. Envir. Microbiol., April 1, 2006; 72(4): 2864 - 2875. [Abstract] [Full Text] [PDF]

				B. Kreikemeyer, M. Nakata, S. Oehmcke, C. Gschwendtner, J. Normann, and A. Podbielski Streptococcus pyogenes Collagen Type I-binding Cpa Surface Protein: EXPRESSION PROFILE, BINDING CHARACTERISTICS, BIOLOGICAL FUNCTIONS, AND POTENTIAL CLINICAL IMPACT J. Biol. Chem., September 30, 2005; 280(39): 33228 - 33239. [Abstract] [Full Text] [PDF]

				V. Ramachandran, J. D. McArthur, C. E. Behm, C. Gutzeit, M. Dowton, P. K. Fagan, R. Towers, B. Currie, K. S. Sriprakash, and M. J. Walker Two Distinct Genotypes of prtF2, Encoding a Fibronectin Binding Protein, and Evolution of the Gene Family in Streptococcus pyogenes J. Bacteriol., November 15, 2004; 186(22): 7601 - 7609. [Abstract] [Full Text] [PDF]