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J. Biol. Chem., Vol. 279, Issue 16, 16479-16487, April 16, 2004

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Delineation of the Role of the Mre11 Complex in Class Switch Recombination^*

Aleksi Lähdesmäki, A. Malcolm R. Taylor, Krystyna H. Chrzanowska¶, and Qiang Pan-Hammarström||

From the Division of Clinical Immunology, Department of Laboratory Medicine, Karolinska Institutet at Huddinge Hospital, SE-14186 Stockholm, Sweden and Center for Biotechnology, NOVUM, SE-14157 Huddinge, Sweden, The University of Birmingham, Cancer Research UK Institute for Cancer Studies, the Medical School Edghaston, Birmingham, B15 2TT, United Kingdom, and the ^¶Department of Medical Genetics, the Children's Memorial Health Institute, Al. Dzieci Polskich 20, 04-736 Warsaw, Poland

Class switch recombination (CSR) is a region-specific, transcriptionally regulated, nonhomologous recombinational process that is initiated by activation-induced cytidine deaminase (AID). The initial lesions in the switch (S) regions are processed and resolved, leading to a recombination of the two S regions involved. The mechanism involved in the repair and ligation of the broken DNA ends is however still unclear. Here, we describe that switching is less efficient in cells from patients with Mre11 deficiency (Ataxia-Telangiectasia-like disorder, ATLD) and, more importantly, that the switch recombination junctions resulting from the in vivo switching events are aberrant. There was a trend toward an increased usage of microhomology (4 bp) at the switch junctions in both ATLD and Nijmegen breakage syndrome (NBS) patients. However, the DNA ends were not joined as "perfectly" as those from Ataxia-Telangiectasia (A-T) patients and 1–2 bp mutations or insertions were often observed. In switch junctions from ATLD patients, there were fewer base substitutions due to transitions and, most strikingly, the substitutions that occurred most often in controls, C T transitions, never occurred at, or close to, the junctions derived from the ATLD patients. In switch junctions from NBS patients, all base substitutions were observed at the G/C nucleotides, and transitions were preferred. These data suggest that the Mre11-Rad50-Nbs1 complex (Mre11 complex) is involved in the nonhomologous end joining pathway in CSR and that Mre11, Nbs1, and protein mutated in ataxia-telangiectasia (ATM) might have both common and independent roles in this process.

Received for publication, November 24, 2003 , and in revised form, January 20, 2004.

^* This work was supported by the Swedish Research Council, the Swedish Society for Medical Research, the Swedish Doctor Association, Jonas Söderqvists Foundation, and funds from the Karolinska Institute. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^|| To whom correspondence should be addressed: Div. of Clinical Immunology, F79, Karolinska Institute at Huddinge Hospital, SE-14186, Stockholm, Sweden. Tel.: 46-8-6089116; Fax: 46-8-58581390; E-mail: qipa{at}biosci.ki.se.

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