HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 16, 16822-16831, April 16, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/16/16822    most recent M312801200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jeong, J. H. Articles by Dittmer, D. P. Search for Related Content PubMed PubMed Citation Articles by Jeong, J. H. Articles by Dittmer, D. P. Social Bookmarking                      What's this?

Regulation and Autoregulation of the Promoter for the Latency-associated Nuclear Antigen of Kaposi's Sarcoma-associated Herpesvirus^*

Joseph H. Jeong, Joshua Orvis, Jong Wook Kim, Curtis P. McMurtrey, Rolf Renne, and Dirk P. Dittmer||¶

From the University of Oklahoma Health Sciences Center, Department of Microbiology and Immunology, Oklahoma City, Oklahoma 73104 Case Western Reserve University, Cleveland, Ohio 44106 and ^||University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599

Kaposi's sarcoma-associated herpesvirus (KSHV) or human herpesvirus 8 has been established as the etiological agent of Kaposi's sarcoma and certain AIDS-associated lymphomas. KSHV establishes latent infection in these tumors, invariably expressing high levels of the viral latency-associated nuclear antigen (LANA) protein. LANA is necessary and sufficient to maintain the KSHV episome. It also modulates viral and cellular transcription and has been implicated directly in oncogenesis because of its ability to bind to the p53 and pRb tumor suppressor proteins. Previously, we identified the LANA promoter (LANAp) and showed that it was positively regulated by LANA itself. Here, we present a detailed mutational analysis and define cis-acting elements and trans-acting factors for the core LANAp. We found that a downstream promoter element, TATA box, and GC box/Sp1 site at -29 are all individually required for activity. This architecture places LANAp into the small and unusual group of eukaryotic promoters that contain both the downstream promoter element and TATA element but lack a defined initiation site. Furthermore, we demonstrate that LANA regulates its own promoter via its C-terminal domain and does bind to a defined site within the core promoter.

Received for publication, November 24, 2003 , and in revised form, January 20, 2004.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Present address: Dana-Farber Cancer Institute, Harvard University, Boston, MA 02115.

^¶ To whom correspondence should be addressed: University of Oklahoma Health Sciences Center, Dept. of Microbiology and Immunology, 940 Stanton L. Young Blvd., Oklahoma City, OK 73104. Tel.: 405-271-2133 (ext. 46645); Fax: 405-271-3117; E-mail: dirk-dittmer{at}ouhsc.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. Lu, S. C. Verma, M. Murakami, Q. Cai, P. Kumar, B. Xiao, and E. S. Robertson Latency-Associated Nuclear Antigen of Kaposi's Sarcoma-Associated Herpesvirus (KSHV) Upregulates Survivin Expression in KSHV-Associated B-Lymphoma Cells and Contributes to Their Proliferation J. Virol., July 15, 2009; 83(14): 7129 - 7141. [Abstract] [Full Text] [PDF]

				M. I. Niesen, A. R. Osborne, W. R. Lagor, H. Zhang, K. Kazemfar, G. C. Ness, and G. Blanck Technological advances in the study of HLA-DRA promoter regulation: Extending the functions of CIITA, Oct-1, Rb, and RFX Acta Biochim Biophys Sin, March 1, 2009; 41(3): 198 - 205. [Abstract] [Full Text] [PDF]

				K. Lan, S. C. Verma, M. Murakami, B. Bajaj, R. Kaul, and E. S. Robertson Kaposi's sarcoma herpesvirus-encoded latency-associated nuclear antigen stabilizes intracellular activated Notch by targeting the Sel10 protein PNAS, October 9, 2007; 104(41): 16287 - 16292. [Abstract] [Full Text] [PDF]

				R. L. Skalsky, J. Hu, and R. Renne Analysis of Viral cis Elements Conferring Kaposi's Sarcoma-Associated Herpesvirus Episome Partitioning and Maintenance J. Virol., September 15, 2007; 81(18): 9825 - 9837. [Abstract] [Full Text] [PDF]

				Q. Cai, K. Lan, S. C. Verma, H. Si, D. Lin, and E. S. Robertson Kaposi's Sarcoma-Associated Herpesvirus Latent Protein LANA Interacts with HIF-1{alpha} To Upregulate RTA Expression during Hypoxia: Latency Control under Low Oxygen Conditions. J. Virol., August 1, 2006; 80(16): 7965 - 7975. [Abstract] [Full Text] [PDF]

				S. Matsumura, Y. Fujita, E. Gomez, N. Tanese, and A. C. Wilson Activation of the Kaposi's Sarcoma-Associated Herpesvirus Major Latency Locus by the Lytic Switch Protein RTA (ORF50) J. Virol., July 1, 2005; 79(13): 8493 - 8505. [Abstract] [Full Text] [PDF]

				K. Lan, D. A. Kuppers, S. C. Verma, N. Sharma, M. Murakami, and E. S. Robertson Induction of Kaposi's Sarcoma-Associated Herpesvirus Latency-Associated Nuclear Antigen by the Lytic Transactivator RTA: a Novel Mechanism for Establishment of Latency J. Virol., June 15, 2005; 79(12): 7453 - 7465. [Abstract] [Full Text] [PDF]

				K. Lan, D. A. Kuppers, and E. S. Robertson Kaposi's Sarcoma-Associated Herpesvirus Reactivation Is Regulated by Interaction of Latency-Associated Nuclear Antigen with Recombination Signal Sequence-Binding Protein J{kappa}, the Major Downstream Effector of the Notch Signaling Pathway J. Virol., March 15, 2005; 79(6): 3468 - 3478. [Abstract] [Full Text] [PDF]

				H. M. Coleman, S. Efstathiou, and P. G. Stevenson Transcription of the murine gammaherpesvirus 68 ORF73 from promoters in the viral terminal repeats J. Gen. Virol., March 1, 2005; 86(3): 561 - 574. [Abstract] [Full Text] [PDF]

				F.-Q. An, N. Compitello, E. Horwitz, M. Sramkoski, E. S. Knudsen, and R. Renne The Latency-associated Nuclear Antigen of Kaposi's Sarcoma-associated Herpesvirus Modulates Cellular Gene Expression and Protects Lymphoid Cells from p16 INK4A-induced Cell Cycle Arrest J. Biol. Chem., February 4, 2005; 280(5): 3862 - 3874. [Abstract] [Full Text] [PDF]

				V. Srinivasan, T. Komatsu, M. E. Ballestas, and K. M. Kaye Definition of Sequence Requirements for Latency-Associated Nuclear Antigen 1 Binding to Kaposi's Sarcoma-Associated Herpesvirus DNA J. Virol., December 15, 2004; 78(24): 14033 - 14038. [Abstract] [Full Text] [PDF]