HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 17, 17361-17365, April 23, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/17/17361    most recent M314104200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sambade, M. Articles by Kane, P. M. Search for Related Content PubMed PubMed Citation Articles by Sambade, M. Articles by Kane, P. M. Social Bookmarking                      What's this?

The Yeast Vacuolar Proton-translocating ATPase Contains a Subunit Homologous to the Manduca sexta and Bovine e Subunits That Is Essential for Function^*

Maria Sambade and Patricia M. Kane

From the Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, New York 13210

The yeast cwh36 mutant was identified in a screen for yeast mutants exhibiting a Vma^- phenotype suggestive of loss of vacuolar proton-translocating ATPase (V-ATPase) activity. The mutation disrupts two genes, CWH36 and a recently identified open reading frame on the opposite strand, YCL005W-A. We demonstrate that disruption of YCL005W-A is entirely responsible for the Vma^- growth phenotype of the cwh36 mutant. YCL005W-A encodes a homolog of proteins associated with the Manduca sexta and bovine chromaffin granule V-ATPase. The functional significance of these proteins for V-ATPase activity had not been tested, but we show that the protein encoded by YCL005W-A, which we call Vma9p, is essential for V-ATPase activity in yeast. Vma9p is localized to the vacuole but fails to reach the vacuole in a mutant lacking one of the integral membrane subunits of the V-ATPase. Vma9p is associated with the yeast V-ATPase complex in vacuolar membranes, as demonstrated by co-immunoprecipitation with known V-ATPase subunits and glycerol gradient fractionation of solubilized vacuolar membranes. Based on this evidence, we propose that Vma9p is a genuine subunit of the yeast V-ATPase and that e subunits may be a functionally essential part of all eukaryotic V-ATPases.

Received for publication, December 23, 2003 , and in revised form, January 30, 2004.

^* This work was supported by National Institutes of Health Grant R01 GM50322 (to P. M. K.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: 315-464-8742; Fax: 315-464-8750; E-mail: kanepm{at}upstate.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Y. Su, K. G. Blake-Palmer, S. Sorrell, B. Javid, K. Bowers, A. Zhou, S. H. Chang, S. Qamar, and F. E. Karet Human H+ATPase a4 subunit mutations causing renal tubular acidosis reveal a role for interaction with phosphofructokinase-1 Am J Physiol Renal Physiol, October 1, 2008; 295(4): F950 - F958. [Abstract] [Full Text] [PDF]

				H. Feng, T. Cheng, N. J. Pavlos, K. H. M. Yip, A. Carrello, R. Seeber, K. Eidne, M. H. Zheng, and J. Xu Cytoplasmic Terminus of Vacuolar Type Proton Pump Accessory Subunit Ac45 Is Required for Proper Interaction with V0 Domain Subunits and Efficient Osteoclastic Bone Resorption J. Biol. Chem., May 9, 2008; 283(19): 13194 - 13204. [Abstract] [Full Text] [PDF]

				W. Fei, G. Alfaro, B.-P. Muthusamy, Z. Klaassen, T. R. Graham, H. Yang, and C. T. Beh Genome-Wide Analysis of Sterol-Lipid Storage and Trafficking in Saccharomyces cerevisiae Eukaryot. Cell, February 1, 2008; 7(2): 401 - 414. [Abstract] [Full Text] [PDF]

				Y. Wang, D. J. Cipriano, and M. Forgac Arrangement of Subunits in the Proteolipid Ring of the V-ATPase J. Biol. Chem., November 23, 2007; 282(47): 34058 - 34065. [Abstract] [Full Text] [PDF]

				S. R. Davis-Kaplan, M. A. Compton, A. R. Flannery, D. M. Ward, J. Kaplan, T. H. Stevens, and L. A. Graham PKR1 Encodes an Assembly Factor for the Yeast V-Type ATPase J. Biol. Chem., October 20, 2006; 281(42): 32025 - 32035. [Abstract] [Full Text] [PDF]

				N. Ochotny, A. Van Vliet, N. Chan, Y. Yao, M. Morel, N. Kartner, H. P. von Schroeder, J. N. M. Heersche, and M. F. Manolson Effects of Human a3 and a4 Mutations That Result in Osteopetrosis and Distal Renal Tubular Acidosis on Yeast V-ATPase Expression and Activity J. Biol. Chem., September 8, 2006; 281(36): 26102 - 26111. [Abstract] [Full Text] [PDF]

				M. A. Compton, L. A. Graham, and T. H. Stevens Vma9p (Subunit e) Is an Integral Membrane V0 Subunit of the Yeast V-ATPase J. Biol. Chem., June 2, 2006; 281(22): 15312 - 15319. [Abstract] [Full Text] [PDF]

				P. M. Kane The Where, When, and How of Organelle Acidification by the Yeast Vacuolar H+-ATPase Microbiol. Mol. Biol. Rev., March 1, 2006; 70(1): 177 - 191. [Abstract] [Full Text] [PDF]

				M. Liu, M. Tarsio, C. M. H. Charsky, and P. M. Kane Structural and Functional Separation of the N- and C-terminal Domains of the Yeast V-ATPase Subunit H J. Biol. Chem., November 4, 2005; 280(44): 36978 - 36985. [Abstract] [Full Text] [PDF]

				M. Sambade, M. Alba, A. M. Smardon, R. W. West, and P. M. Kane A Genomic Screen for Yeast Vacuolar Membrane ATPase Mutants Genetics, August 1, 2005; 170(4): 1539 - 1551. [Abstract] [Full Text] [PDF]

				T. Inoue and M. Forgac Cysteine-mediated Cross-linking Indicates That Subunit C of the V-ATPase Is in Close Proximity to Subunits E and G of the V1 Domain and Subunit a of the V0 Domain J. Biol. Chem., July 29, 2005; 280(30): 27896 - 27903. [Abstract] [Full Text] [PDF]

				T. Wassmer, M. Froissard, H. Plattner, R. Kissmehl, and J. Cohen The vacuolar proton-ATPase plays a major role in several membrane-bounded organelles in Paramecium J. Cell Sci., July 1, 2005; 118(13): 2813 - 2825. [Abstract] [Full Text] [PDF]

				G. J. Hermann, L. K. Schroeder, C. A. Hieb, A. M. Kershner, B. M. Rabbitts, P. Fonarev, B. D. Grant, and J. R. Priess Genetic Analysis of Lysosomal Trafficking in Caenorhabditis elegans Mol. Biol. Cell, July 1, 2005; 16(7): 3273 - 3288. [Abstract] [Full Text] [PDF]

				M. A. Owegi, A. L. Carenbauer, N. M. Wick, J. F. Brown, K. L. Terhune, S. A. Bilbo, R. S. Weaver, R. Shircliff, N. Newcomb, and K. J. Parra-Belky Mutational Analysis of the Stator Subunit E of the Yeast V-ATPase J. Biol. Chem., May 6, 2005; 280(18): 18393 - 18402. [Abstract] [Full Text] [PDF]

				A. N. Smith, F. Jouret, S. Bord, K. J. Borthwick, R. S. Al-Lamki, C. A. Wagner, D. C. Ireland, V. Cormier-Daire, A. Frattini, A. Villa, et al. Vacuolar H+-ATPase d2 Subunit: Molecular Characterization, Developmental Regulation, and Localization to Specialized Proton Pumps in Kidney and Bone J. Am. Soc. Nephrol., May 1, 2005; 16(5): 1245 - 1256. [Abstract] [Full Text] [PDF]

				E. Shao and M. Forgac Involvement of the Nonhomologous Region of Subunit A of the Yeast V-ATPase in Coupling and in Vivo Dissociation J. Biol. Chem., November 19, 2004; 279(47): 48663 - 48670. [Abstract] [Full Text] [PDF]