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J. Biol. Chem., Vol. 279, Issue 17, 17411-17417, April 23, 2004

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GGA1 Interacts with the Adaptor Protein AP-1 through a WNSF Sequence in Its Hinge Region^*

Hongdong Bai, Balraj Doray, and Stuart Kornfeld

From the Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

The Golgi-associated -adaptin-related ADP-ribosylation factor-binding proteins (GGAs) are critical components of the transport machinery that mediates the trafficking of the mannose 6-phosphate receptors and associated cargo from the trans-Golgi network to the endosomes. The GGAs colocalize in vivo with the clathrin adaptor protein AP-1 and bind to AP-1 in vitro, suggesting that the two proteins may cooperate in packaging the mannose 6-phosphate receptors into clathrin-coated vesicles at the trans-Golgi network. Here, we demonstrate that the sequence, ³⁸²WNSF³⁸⁵, in the hinge region of GGA1 mediates its interaction with the AP-1 -ear. The Trp and Phe constitute critical amino acids in this interaction. The binding of Rabaptin5 to the AP-1 -ear, which occurs through a FXX motif, is inhibited by a peptide encoding the GGA1 ³⁸²WNSF³⁸⁵ sequence. Moreover, mutations in the AP-1 -ear that abolish its interaction with Rabaptin5 also preclude its association with GGA1. These results suggest that the GGA1 WXXF-type and Rabaptin5 FXX-type motifs bind to the same or highly overlapping sites in the AP-1 -ear. This binding is modulated by residues adjacent to the core motifs.

Received for publication, February 2, 2004 , and in revised form, February 18, 2004.

^* This work was supported in part by National Institutes of Health Grant R01 CA-08759 (to S. K.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Washington University School of Medicine; Division of Hematology, 660 S. Euclid Ave., Campus Box 8125, St. Louis, MO 63110. Tel.: 314-362-8803; Fax: 314-362-8826; E-mail: skornfel{at}im.wustl.edu.

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