JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Originally published In Press as doi:10.1074/jbc.M308136200 on February 19, 2004

J. Biol. Chem., Vol. 279, Issue 18, 18694-18700, April 30, 2004
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An addition or correction has been published
Right arrow All Versions of this Article:
279/18/18694    most recent
M308136200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liu, Y.
Right arrow Articles by Phang, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, Y.
Right arrow Articles by Phang, J. M.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Polyoma Enhancer Activator 3, an Ets Transcription Factor, Mediates the Induction of Cyclooxygenase-2 by Nitric Oxide in Colorectal Cancer Cells*

Yongmin Liu{ddagger}, Gregory L. Borchert§, and James M. Phang{ddagger}

From the {ddagger}Metabolism & Cancer Susceptibility Section, Laboratory of Comparative Carcinogenesis, Center for Cancer Research, NCI, National Institutes of Health, Frederick, Maryland 21702 and the §Basic Research Program, Science Applications International Corporation-Frederick, Inc., Maryland 21702

Abundant evidence supports the role of cyclooxygenase-2 (COX-2) in colorectal cancer. Nitric oxide (NO), a pro-inflammatory signaling factor, may regulate COX-2 expression and activity thereby linking hyper-inflammatory states to cancer susceptibility. Previously we showed that NO induced COX-2 expression. Although NO also activated the {beta}-catenin·T-cell factor/lymphocyte enhancing factor transcriptional pathway, a direct causal link between this pathway and COX-2 expression was not demonstrated. In this current study, we focused on NO-induced transcriptional activity and elucidated its role in COX-2 expression. NO donors stimulated the expression of peroxisome proliferator-activated receptor-{delta} and c-myc, both downstream genes of {beta}-catenin. They also induced the expression of polyoma enhancer activator 3 (PEA3) and increased its DNA-binding activity. To establish a role for PEA3 to {beta}-catenin-induced COX-2, we transfected RKO cells with {beta}-catenin and found that {beta}-catenin increased PEA3 expression. Also, there was higher PEA3 in immortal mouse colon epithelium cells (ApcMin/+) compared with young adult mouse colon cells (Apc+/+). Luciferase reporter assays revealed that, although several transcription factors/coactivator, acting alone or in synergistic combination, induced COX-2 promoter activity, PEA3 was one of the most potent. Interestingly, NO from NO donors or generated endogenously from transfected inducible nitric-oxide synthase, increased PEA3/p300-induced COX-2 promoter activity. We also found that an ETS site (-75/-72) and the NF-IL6 site were responsible for COX-2 activity induced by PEA3, PEA3/p300, and NO. Taken together, our results demonstrated that NO through {beta}-catenin signaling stimulated PEA3 to increase COX-2 activity. In addition, NO augmented the synergistic interaction between PEA3 and CBP/p300.


Received for publication, July 25, 2003 , and in revised form, February 17, 2004.

* This work was supported by the NCI, National Institutes of Health, under contract NO1-CO-12400. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: NCI, National Institutes of Health, Bldg. 538, Rm. 144, Frederick, MD 21702. Tel.: 301-846-5367; Fax: 301-846-6093; E-mail: phang{at}ncifcrf.gov.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Y. H. Lee, Y. J. Suzuki, A. J. Griffin, and R. M. Day
Hepatocyte growth factor regulates cyclooxygenase-2 expression via {beta}-catenin, Akt, and p42/p44 MAPK in human bronchial epithelial cells
Am J Physiol Lung Cell Mol Physiol, April 1, 2008; 294(4): L778 - L786.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
K. Subbaramaiah and A. J. Dannenberg
Cyclooxygenase-2 Transcription Is Regulated by Human Papillomavirus 16 E6 and E7 Oncoproteins: Evidence of a Corepressor/Coactivator Exchange
Cancer Res., April 15, 2007; 67(8): 3976 - 3985.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
L. H. Kasper, T. Fukuyama, M. A. Biesen, F. Boussouar, C. Tong, A. de Pauw, P. J. Murray, J. M. A. van Deursen, and P. K. Brindle
Conditional Knockout Mice Reveal Distinct Functions for the Global Transcriptional Coactivators CBP and p300 in T-Cell Development
Mol. Cell. Biol., February 1, 2006; 26(3): 789 - 809.
[Abstract] [Full Text] [PDF]


Home page
CarcinogenesisHome page
J. Hong, S. Sang, H.-J. Park, S. J. Kwon, N. Suh, M.-T. Huang, C.-T. Ho, and C. S. Yang
Modulation of arachidonic acid metabolism and nitric oxide synthesis by garcinol and its derivatives
Carcinogenesis, February 1, 2006; 27(2): 278 - 286.
[Abstract] [Full Text] [PDF]


Home page
CarcinogenesisHome page
K. Nosho, M. Yoshida, H. Yamamoto, H. Taniguchi, Y. Adachi, M. Mikami, Y. Hinoda, and K. Imai
Association of Ets-related transcriptional factor E1AF expression with overexpression of matrix metalloproteinases, COX-2 and iNOS in the early stage of colorectal carcinogenesis
Carcinogenesis, May 1, 2005; 26(5): 892 - 899.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
V. Firlej, B. Bocquet, X. Desbiens, Y. de Launoit, and A. Chotteau-Lelievre
Pea3 Transcription Factor Cooperates with USF-1 in Regulation of the Murine bax Transcription without Binding to an Ets-binding Site
J. Biol. Chem., January 14, 2005; 280(2): 887 - 898.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.