| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 279, Issue 18, 19149-19156, April 30, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Ophthalmology, Harvard Medical School and the Massachusetts Eye and Ear Infirmary, Boston, Massachusetts 02114
Phosducin is a photoreceptor-specific protein known to interact with the 
subunits of G proteins. In pursuit of the function of phosducin, we tested the hypothesis that it regulates the light-driven translocation of G protein transducin from the outer segments of rod photoreceptors to other compartments of the rod cell. Transducin translocation has been previously shown to contribute to rod adaptation to bright illumination, yet the molecular mechanisms underlying the translocation phenomenon remain unknown. In this study we provide two major lines of evidence in support of the role of phosducin in transducin translocation. First, we have demonstrated that transducin subunits interact with phosducin along their entire intracellular translocation route, as evident from their co-precipitation in serial tangential sections from light-adapted but not dark-adapted retinas. Second, we generated a phosducin knockout mouse and found that the degree of light-driven transducin translocation in the rods of these mice was significantly reduced as compared with that observed in the rods of wild type animals. In knockout animals the translocation of transducin subunits was affected to a larger degree than the translocation of the 
subunit. We also found that the amount of phosducin in rods is sufficient to interact with practically all of the transducin present in these cells and that the subcellular distribution of phosducin is consistent with that of a soluble protein evenly distributed throughout the entire rod cytoplasm. Together, these data indicate that phosducin binding to transducin subunits facilitates transducin translocation. We suggest that the mechanism of phosducin action is based on the reduction of transducin affinity to the membranes of rod outer segments, achieved by keeping the transducin subunits apart from the subunit. This increased solubility of transducin would make it more susceptible to translocation from the outer segments.
Received for publication, October 8, 2003 , and in revised form, January 26, 2004.
* This work was supported by National Institutes of Health Grant EY-10336 (to V. Y. A.), a grant from the Massachusetts Lions Eye Research Fund (to V. Y. A.), grants from Knights Templar Eye Foundation (to M. S. and K. J. S.), and United States Civilian Research and Development Foundation Grant RB1-217 (to V. I. G. and V. Y. A.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Permanent address: Institute of Evolutionary Physiology and Biochemistry, St. Petersburg, Russia 194223.
To whom correspondence should be addressed: Dept. of Ophthalmology, Harvard Medical School, Howe Labs/MEEI, 243 Charles St., Boston MA 02114. Tel.: 617-573-4371; Fax: 617-573-4290; E-mail: vadim_arshavsky{at}meei.harvard.edu.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Kosloff, E. Alexov, V. Y. Arshavsky, and B. Honig Electrostatic and Lipid Anchor Contributions to the Interaction of Transducin with Membranes: MECHANISTIC IMPLICATIONS FOR ACTIVATION AND TRANSLOCATION J. Biol. Chem., November 7, 2008; 283(45): 31197 - 31207. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Baker, M. Haeri, P. Yoo, S. M. Gospe III, N. P. Skiba, B. E. Knox, and V. Y. Arshavsky The outer segment serves as a default destination for the trafficking of membrane proteins in photoreceptors J. Cell Biol., November 3, 2008; 183(3): 485 - 498. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Cao, H. Song, H. Okawa, A. P. Sampath, M. Sokolov, and K. A. Martemyanov Targeting of RGS7/G{beta}5 to the Dendritic Tips of ON-Bipolar Cells Is Independent of Its Association with Membrane Anchor R7BP J. Neurosci., October 8, 2008; 28(41): 10443 - 10449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. Lobanova, S. Finkelstein, R. Herrmann, Y.-M. Chen, C. Kessler, N. A. Michaud, L. H. Trieu, K. J. Strissel, M. E. Burns, and V. Y. Arshavsky Transducin {gamma}-Subunit Sets Expression Levels of {alpha}- and {beta}-Subunits and Is Crucial for Rod Viability J. Neurosci., March 26, 2008; 28(13): 3510 - 3520. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Sobierajska, H. Fabczak, and S. Fabczak Phosducin interacts with the G-protein {beta}{gamma}-dimer of ciliate protozoan Blepharisma japonicum upon illumination J. Exp. Biol., December 1, 2007; 210(23): 4213 - 4223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Krispel, M. Sokolov, Y.-M. Chen, H. Song, R. Herrmann, V. Y. Arshavsky, and M. E. Burns Phosducin Regulates the Expression of Transducin {beta}{gamma} Subunits in Rod Photoreceptors and Does Not Contribute to Phototransduction Adaptation J. Gen. Physiol., August 27, 2007; 130(3): 303 - 312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Song, M. Belcastro, E. J. Young, and M. Sokolov Compartment-specific Phosphorylation of Phosducin in Rods Underlies Adaptation to Various Levels of Illumination J. Biol. Chem., August 10, 2007; 282(32): 23613 - 23621. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhang, S. Li, T. Doan, F. Rieke, P. B. Detwiler, J. M. Frederick, and W. Baehr Deletion of PrBP/{delta} impedes transport of GRK1 and PDE6 catalytic subunits to photoreceptor outer segments PNAS, May 22, 2007; 104(21): 8857 - 8862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Rosenzweig, K. S. Nair, J. Wei, Q. Wang, G. Garwin, J. C. Saari, C.-K. Chen, A. V. Smrcka, A. Swaroop, J. Lem, et al. Subunit Dissociation and Diffusion Determine the Subcellular Localization of Rod and Cone Transducins J. Neurosci., May 16, 2007; 27(20): 5484 - 5494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rajala, R. E. Anderson, J.-X. Ma, J. Lem, M. R. Al-Ubaidi, and R. V. S. Rajala G-protein-coupled Receptor Rhodopsin Regulates the Phosphorylation of Retinal Insulin Receptor J. Biol. Chem., March 30, 2007; 282(13): 9865 - 9873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. Lobanova, S. Finkelstein, H. Song, S. H. Tsang, C.-K. Chen, M. Sokolov, N. P. Skiba, and V. Y. Arshavsky Transducin Translocation in Rods Is Triggered by Saturation of the GTPase-Activating Complex J. Neurosci., January 31, 2007; 27(5): 1151 - 1160. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Knopp and H. Ruppel Calcium-Sensitive Downregulation of the Transduction Chain in Rod Photoreceptors of the Rat Retina Biophys. J., August 1, 2006; 91(3): 1078 - 1089. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Partridge, H. L. Puhl III, and S. R. Ikeda Phosducin and Phosducin-like Protein Attenuate G-Protein-Coupled Receptor-Mediated Inhibition of Voltage-Gated Calcium Channels in Rat Sympathetic Neurons Mol. Pharmacol., July 1, 2006; 70(1): 90 - 100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. E. Meyer, T. Joel-Almagor, S. Frechter, B. Minke, and A. Huber Subcellular translocation of the eGFP-tagged TRPL channel in Drosophila photoreceptors requires activation of the phototransduction cascade J. Cell Sci., June 15, 2006; 119(12): 2592 - 2603. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Hauck, P. A. R. Ekstrom, P. Ahuja-Jensen, S. Suppmann, F. Paquet-Durand, T. van Veen, and M. Ueffing Differential Modification of Phosducin Protein in Degenerating rd1 Retina Is Associated with Constitutively Active Ca2+/Calmodulin Kinase II in Rod Outer Segments Mol. Cell. Proteomics, February 1, 2006; 5(2): 324 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Strissel, M. Sokolov, L. H. Trieu, and V. Y. Arshavsky Arrestin Translocation Is Induced at a Critical Threshold of Visual Signaling and Is Superstoichiometric to Bleached Rhodopsin J. Neurosci., January 25, 2006; 26(4): 1146 - 1153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Kerov, D. Chen, M. Moussaif, Y.-J. Chen, C.-K. Chen, and N. O. Artemyev Transducin Activation State Controls Its Light-dependent Translocation in Rod Photoreceptors J. Biol. Chem., December 9, 2005; 280(49): 41069 - 41076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Elia, S. Frechter, Y. Gedi, B. Minke, and Z. Selinger Excess of G{beta}e over Gq{alpha}e in vivo prevents dark, spontaneous activity of Drosophila photoreceptors J. Cell Biol., November 7, 2005; 171(3): 517 - 526. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Peterson, W. Orisme, J. Fellows, J. H. McDowell, C. L. Shelamer, D. R. Dugger, and W. C. Smith A Role for Cytoskeletal Elements in the Light-Driven Translocation of Proteins in Rod Photoreceptors Invest. Ophthalmol. Vis. Sci., November 1, 2005; 46(11): 3988 - 3998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Strissel, P. V. Lishko, L. H. Trieu, M. J. Kennedy, J. B. Hurley, and V. Y. Arshavsky Recoverin Undergoes Light-dependent Intracellular Translocation in Rod Photoreceptors J. Biol. Chem., August 12, 2005; 280(32): 29250 - 29255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Y. Lee, C. D. Thulin, and B. M. Willardson Site-specific Phosphorylation of Phosducin in Intact Retina: DYNAMICS OF PHOSPHORYLATION AND EFFECTS ON G PROTEIN {beta}{gamma} DIMER BINDING J. Biol. Chem., December 24, 2004; 279(52): 54008 - 54017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Giessl, A. Pulvermuller, P. Trojan, J. H. Park, H.-W. Choe, O. P. Ernst, K. P. Hofmann, and U. Wolfrum Differential Expression and Interaction with the Visual G-protein Transducin of Centrin Isoforms in Mammalian Photoreceptor Cells J. Biol. Chem., December 3, 2004; 279(49): 51472 - 51481. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
