HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 19, 19634-19642, May 7, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/19/19634    most recent M311647200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kethidi, D. R. Articles by Palli, S. R. Search for Related Content PubMed PubMed Citation Articles by Kethidi, D. R. Articles by Palli, S. R. Social Bookmarking                      What's this?

Identification and Characterization of a Juvenile Hormone (JH) Response Region in the JH Esterase Gene from the Spruce Budworm, Choristoneura fumiferana^*

Damodar R. Kethidi, Srini C. Perera, S. Zheng, Qi-Li Feng, Peter Krell¶, Arthur Retnakaran, and Subba R. Palli||

From the Department of Entomology, College of Agriculture, University of Kentucky, Kentucky 40546, the Great Lakes Forestry Centre, Canadian Forestry Service, 1219 Queen Street East, Sault Ste. Marie, Ontario P6A 2E5, Canada, and the ^¶Department of Microbiology, University of Guelph, Ontario NIG 2W1, Canada

Using a differential display of mRNA technique we discovered that the juvenile hormone (JH) esterase gene (Cfjhe) from Choristoneura fumiferana is directly induced by juvenile hormone I (JH I), and the JH I induction is suppressed by 20-hydroxyecdysone (20E). To study the mechanism of action of these two hormones in the regulation of expression of this gene, we cloned the 1270-bp promoter region of the Cfjhe gene and identified a 30-bp region that is located between –604 and –574 and is sufficient to support both JH I induction and 20E suppression. This 30-bp region contains two conserved hormone response element half-sites separated by a 4-nucleotide spacer similar to the direct repeat 4 element and is designated as a putative juvenile hormone response element (JHRE). In CF-203 cells, a luciferase reporter placed under the control of JHRE and a minimal promoter was induced by JH I in a dose- and time-dependent manner. Moreover, 20E suppressed this JH I-induced luciferase activity in a dose- and time-dependent manner. Nuclear proteins isolated from JH I-treated CF-203 cells bound to JHRE and the binding was competed by a 100-fold excess of the cold probe but not by 100-fold excess of double-stranded oligonucleotides of unrelated sequence. JH I induced/modified nuclear proteins prior to their binding to JHRE and 20E suppressed this JH I induction/modification. These results suggest that the 30-bp JHRE identified in the Cfjhe gene promoter is sufficient to support JH induction and 20E suppression of the Cfjhe gene.

Received for publication, October 23, 2003 , and in revised form, February 13, 2004.

^* This work was supported by the Canadian Biotechnology Strategy fund, Genome Canada, and by the Cooperative State Research, Education, and Extension Service of the United States Department of Agriculture. This is contribution number 04-08-025 from the Kentucky Agricultural Experimental Station. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^|| To whom correspondence should be addressed: Dept. of Entomology, College of Agriculture, University of Kentucky, Lexington, KY 40546. Tel.: 859-257-4962; Fax: 859-323-1120; E-mail: rpalli{at}uky.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Y. Li, Z. Zhang, G. E. Robinson, and S. R. Palli Identification and Characterization of a Juvenile Hormone Response Element and Its Binding Proteins J. Biol. Chem., December 28, 2007; 282(52): 37605 - 37617. [Abstract] [Full Text] [PDF]

				A. Tan, H. Tanaka, T. Tamura, and T. Shiotsuki Precocious metamorphosis in transgenic silkworms overexpressing juvenile hormone esterase PNAS, August 16, 2005; 102(33): 11751 - 11756. [Abstract] [Full Text] [PDF]