HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 19, 20471-20479, May 7, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/19/20471    most recent M400798200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rao, S. K. Articles by Andrews, N. W. Search for Related Content PubMed PubMed Citation Articles by Rao, S. K. Articles by Andrews, N. W. Social Bookmarking                      What's this?

Identification of SNAREs Involved in Synaptotagmin VII-regulated Lysosomal Exocytosis^*

Swathi K. Rao, Chau Huynh¶, Veronique Proux-Gillardeaux||, Thierry Galli||, and Norma W. Andrews**

From the Section of Microbial Pathogenesis and Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06511 and ^||Membrane Traffic and Neuronal Plasticity, INSERM U536, Institut du Fer-à-Moulin, 75005 Paris, France

Ca²⁺-regulated exocytosis of lysosomes has been recognized recently as a ubiquitous process, important for the repair of plasma membrane wounds. Lysosomal exocytosis is regulated by synaptotagmin VII, a member of the synaptotagmin family of Ca²⁺-binding proteins localized on lysosomes. Here we show that Ca²⁺-dependent interaction of the synaptotagmin VII C₂A domain with SNAP-23 is facilitated by syntaxin 4. Specific interactions also occurred in cell lysates between the plasma membrane t-SNAREs SNAP-23 and syntaxin 4 and the lysosomal v-SNARE TI-VAMP/VAMP7. Following cytosolic Ca²⁺ elevation, SDS-resistant complexes containing SNAP-23, syntaxin 4, and TI-VAMP/VAMP7 were detected on membrane fractions. Lysosomal exocytosis was inhibited by the SNARE domains of syntaxin 4 and TI-VAMP/VAMP7 and by cleavage of SNAP-23 with botulinum neurotoxin E, thereby functionally implicating these SNAREs in Ca²⁺-regulated exocytosis of conventional lysosomes.

Received for publication, January 23, 2004 , and in revised form, February 26, 2004.

^* This work was supported in part by grants from the National Institutes of Health, a Burroughs Wellcome Scholar award (to N. W. A.), and grants from ARC and Human Frontiers Science Program (to T. G.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Supported by NRSA Grant GM07499-24 and submitted this work to fulfill in part the requirements for the degree of Doctor of Philosophy at Yale University.

^¶ Burroughs Welcome fellow of the Life Sciences Research Foundation.

^** To whom correspondence should be addressed: Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Ave., New Haven, CT 06536. Tel.: 203-737-2410; Fax: 203-737-2630; E-mail: norma.andrews{at}yale.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Chaineau, L. Danglot, V. Proux-Gillardeaux, and T. Galli Role of HRB in Clathrin-dependent Endocytosis J. Biol. Chem., December 5, 2008; 283(49): 34365 - 34373. [Abstract] [Full Text] [PDF]

				E. Cocucci, G. Racchetti, M. Rupnik, and J. Meldolesi The regulated exocytosis of enlargeosomes is mediated by a SNARE machinery that includes VAMP4 J. Cell Sci., September 15, 2008; 121(18): 2983 - 2991. [Abstract] [Full Text] [PDF]

				D. Li, N. Ropert, A. Koulakoff, C. Giaume, and M. Oheim Lysosomes Are the Major Vesicular Compartment Undergoing Ca2+-Regulated Exocytosis from Cortical Astrocytes J. Neurosci., July 23, 2008; 28(30): 7648 - 7658. [Abstract] [Full Text] [PDF]

				A. Yaradanakul, T.-M. Wang, V. Lariccia, M.-J. Lin, C. Shen, X. Liu, and D. W. Hilgemann Massive Ca-induced Membrane Fusion and Phospholipid Changes Triggered by Reverse Na/Ca Exchange in BHK Fibroblasts J. Gen. Physiol., July 1, 2008; 132(1): 29 - 50. [Abstract] [Full Text] [PDF]

				S. Chen and J. T. Barbieri Multiple Pocket Recognition of SNAP25 by Botulinum Neurotoxin Serotype E J. Biol. Chem., August 31, 2007; 282(35): 25540 - 25547. [Abstract] [Full Text] [PDF]

				T. M. Casey, J. L. Meade, and E. W. Hewitt Organelle Proteomics: Identification of the Exocytic Machinery Associated with the Natural Killer Cell Secretory Lysosome Mol. Cell. Proteomics, May 1, 2007; 6(5): 767 - 780. [Abstract] [Full Text] [PDF]

				T. Tsuboi and M. Fukuda Synaptotagmin VII modulates the kinetics of dense-core vesicle exocytosis in PC12 cells Genes Cells, April 1, 2007; 12(4): 511 - 519. [Abstract] [Full Text] [PDF]

				A. Michihara, K. Toda, M. Suenobu, K. Akasaki, and H. Tsuji Decrease of Cholesterol in Mouse Melanoma Causes Secretion of Lysosomal Enzymes J. Biochem., February 1, 2007; 141(2): 239 - 250. [Abstract] [Full Text] [PDF]

				A. Scheuber, R. Rudge, L. Danglot, G. Raposo, T. Binz, J.-C. Poncer, and T. Galli Loss of AP-3 function affects spontaneous and evoked release at hippocampal mossy fiber synapses PNAS, October 31, 2006; 103(44): 16562 - 16567. [Abstract] [Full Text] [PDF]

				F. Mollinedo, J. Calafat, H. Janssen, B. Martin-Martin, J. Canchado, S. M. Nabokina, and C. Gajate Combinatorial SNARE Complexes Modulate the Secretion of Cytoplasmic Granules in Human Neutrophils. J. Immunol., September 1, 2006; 177(5): 2831 - 2841. [Abstract] [Full Text] [PDF]

				G. van Niel, I. Porto-Carreiro, S. Simoes, and G. Raposo Exosomes: a common pathway for a specialized function. J. Biochem., July 1, 2006; 140(1): 13 - 21. [Abstract] [Full Text] [PDF]

				N. L. Washington and S. Ward FER-1 regulates Ca2+-mediated membrane fusion during C. elegans spermatogenesis J. Cell Sci., June 15, 2006; 119(12): 2552 - 2562. [Abstract] [Full Text] [PDF]

				R. M. E. Arantes and N. W. Andrews A role for synaptotagmin VII-regulated exocytosis of lysosomes in neurite outgrowth from primary sympathetic neurons. J. Neurosci., April 26, 2006; 26(17): 4630 - 4637. [Abstract] [Full Text] [PDF]

				J.-H. Tian, Z.-X. Wu, M. Unzicker, L. Lu, Q. Cai, C. Li, C. Schirra, U. Matti, D. Stevens, C. Deng, et al. The Role of Snapin in Neurosecretion: Snapin Knock-Out Mice Exhibit Impaired Calcium-Dependent Exocytosis of Large Dense-Core Vesicles in Chromaffin Cells J. Neurosci., November 9, 2005; 25(45): 10546 - 10555. [Abstract] [Full Text] [PDF]

				N. E. Vlahakis and R. D. Hubmayr Cellular Stress Failure in Ventilator-injured Lungs Am. J. Respir. Crit. Care Med., June 15, 2005; 171(12): 1328 - 1342. [Abstract] [Full Text] [PDF]

				N. W. Andrews Membrane Resealing: Synaptotagmin VII Keeps Running the Show Sci. Signal., May 3, 2005; 2005(282): pe19 - pe19. [Abstract] [Full Text] [PDF]

				M. Fukuda, E. Kanno, M. Satoh, C. Saegusa, and A. Yamamoto Synaptotagmin VII Is Targeted to Dense-core Vesicles and Regulates Their Ca2+-dependent Exocytosis in PC12 Cells J. Biol. Chem., December 10, 2004; 279(50): 52677 - 52684. [Abstract] [Full Text] [PDF]

				V. K. Randhawa, F. S.L. Thong, D. Y. Lim, D. Li, R. R. Garg, R. Rudge, T. Galli, A. Rudich, and A. Klip Insulin and Hypertonicity Recruit GLUT4 to the Plasma Membrane of Muscle Cells by Using N-Ethylmaleimide-sensitive Factor-dependent SNARE Mechanisms but Different v-SNAREs: Role of TI-VAMP Mol. Biol. Cell, December 1, 2004; 15(12): 5565 - 5573. [Abstract] [Full Text] [PDF]

				C. Huynh, D. Roth, D. M. Ward, J. Kaplan, and N. W. Andrews From the Cover: Defective lysosomal exocytosis and plasma membrane repair in Chediak-Higashi/beige cells PNAS, November 30, 2004; 101(48): 16795 - 16800. [Abstract] [Full Text] [PDF]