HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 20, 21046-21054, May 14, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/20/21046    most recent M313370200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zähringer, U. Articles by Dehio, C. Search for Related Content PubMed PubMed Citation Articles by Zähringer, U. Articles by Dehio, C. Social Bookmarking                      What's this?

Structure and Biological Activity of the Short-chain Lipopolysaccharide from Bartonella henselae ATCC 49882^T*

Ulrich Zähringer, Buko Lindner, Yuriy A. Knirel, Willem M. R. van den Akker¶, Rosemarie Hiestand||, Holger Heine, and Christoph Dehio||

From the Research Center Borstel, Leibniz-Center for Medicine and Biosciences, 23845 Borstel, Germany, the ^¶Hubrecht Laboratory, Netherlands Institute for Developmental Biology, 3584 CT Utrecht, The Netherlands, and the ^||Biozentrum of the University of Basel, Division of Molecular Microbiology, 4056 Basel, Switzerland

The facultative intracellular pathogen Bartonella henselae is responsible for a broad range of clinical manifestations, including the formation of vascular tumors as a result of increased proliferation and survival of colonized endothelial cells. This remarkable interaction with endotoxin-sensitive endothelial cells and the apparent lack of septic shock are considered to be due to a reduced endotoxic activity of the B. henselae lipopolysaccharide. Here, we show that B. henselae ATCC 49882^T produces a deep-rough-type lipopolysaccharide devoid of O-chain and report on its complete structure and Toll-like receptor-dependent biological activity. The major short-chain lipopolysaccharide was studied by chemical analyses, electrospray ionization, and matrix-assisted laser desorption/ionization mass spectrometry, as well as by NMR spectroscopy after alkaline deacylation. The carbohydrate portion of the lipopolysaccharide consists of a branched trisaccharide containing a glucose residue attached to position 5 of an -(24)-linked 3-deoxy-D-manno-oct-2-ulosonic acid disaccharide. Lipid A is a pentaacylated -(1'6)-linked 2,3-diamino-2,3-dideoxy-glucose disaccharide 1,4'-bisphosphate with two amide-linked residues each of 3-hydroxydodecanoic and 3-hydroxyhexadecanoic acids and one residue of either 25-hydroxyhexacosanoic or 27-hydroxyoctacosanoic acid that is O-linked to the acyl group at position 2'. The lipopolysaccharide studied activated Toll-like receptor 4 signaling only to a low extent (1,000–10,000-fold lower compared with that of Salmonella enterica sv. Friedenau) and did not activate Toll-like receptor 2. Some unusual structural features of the B. henselae lipopolysaccharide, including the presence of a long-chain fatty acid, which are shared by the lipopolysaccharides of other bacteria causing chronic intracellular infections (e.g. Legionella and Chlamydia), may provide the molecular basis for low endotoxic potency.

Received for publication, December 8, 2003 , and in revised form, January 26, 2004.

^* This work was supported by Deutsche Forschungsgemeinschaft Grants LI-448/1-1 (to B. L. and U. Z.) and ZA 149/3-2 (to U. Z.) and by Swiss National Science Foundation Grant 31-61777.00 (to C. D.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: 49-4537-188462; Fax: 49-4537-188406; E-mail: uzaehr{at}fz-borstel.de.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Kunz, K. Oberle, A. Sander, C. Bogdan, and U. Schleicher Lymphadenopathy in a Novel Mouse Model of Bartonella-Induced Cat Scratch Disease Results from Lymphocyte Immigration and Proliferation and Is Regulated by Interferon-{alpha}/{beta} Am. J. Pathol., April 1, 2008; 172(4): 1005 - 1018. [Abstract] [Full Text] [PDF]

				C. Popa, S. Abdollahi-Roodsaz, L. A. B. Joosten, N. Takahashi, T. Sprong, G. Matera, M. C. Liberto, A. Foca, M. van Deuren, B. J. Kullberg, et al. Bartonella quintana Lipopolysaccharide Is a Natural Antagonist of Toll-Like Receptor 4 Infect. Immun., October 1, 2007; 75(10): 4831 - 4837. [Abstract] [Full Text] [PDF]

				S. M. Dabo, A. W. Confer, B. E. Anderson, and S. Gupta Bartonella henselae Pap31, an Extracellular Matrix Adhesin, Binds the Fibronectin Repeat III13 Module. Infect. Immun., May 1, 2006; 74(5): 2513 - 2521. [Abstract] [Full Text] [PDF]

				W. Vermi, F. Facchetti, E. Riboldi, H. Heine, S. Scutera, S. Stornello, D. Ravarino, P. Cappello, M. Giovarelli, R. Badolato, et al. Role of dendritic cell-derived CXCL13 in the pathogenesis of Bartonella henselae B-rich granuloma Blood, January 15, 2006; 107(2): 454 - 462. [Abstract] [Full Text] [PDF]

				A. M. McCord, A. W. O. Burgess, M. J. Whaley, and B. E. Anderson Interaction of Bartonella henselae with Endothelial Cells Promotes Monocyte/Macrophage Chemoattractant Protein 1 Gene Expression and Protein Production and Triggers Monocyte Migration Infect. Immun., September 1, 2005; 73(9): 5735 - 5742. [Abstract] [Full Text] [PDF]

				V. A.J. Kempf, M. Lebiedziejewski, K. Alitalo, J.-H. Walzlein, U. Ehehalt, J. Fiebig, S. Huber, B. Schutt, C. A. Sander, S. Muller, et al. Activation of Hypoxia-Inducible Factor-1 in Bacillary Angiomatosis: Evidence for a Role of Hypoxia-Inducible Factor-1 in Bacterial Infections Circulation, March 1, 2005; 111(8): 1054 - 1062. [Abstract] [Full Text] [PDF]

				M. Akamine, F. Higa, N. Arakaki, K. Kawakami, K. Takeda, S. Akira, and A. Saito Differential Roles of Toll-Like Receptors 2 and 4 in In Vitro Responses of Macrophages to Legionella pneumophila Infect. Immun., January 1, 2005; 73(1): 352 - 361. [Abstract] [Full Text] [PDF]

				L. L. Stoll, G. M. Denning, and N. L. Weintraub Potential Role of Endotoxin as a Proinflammatory Mediator of Atherosclerosis Arterioscler. Thromb. Vasc. Biol., December 1, 2004; 24(12): 2227 - 2236. [Abstract] [Full Text] [PDF]