JBC Connect with Cosmo for Collagen Detection

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Originally published In Press as doi:10.1074/jbc.M313039200 on March 1, 2004

J. Biol. Chem., Vol. 279, Issue 20, 21327-21333, May 14, 2004
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
279/20/21327    most recent
M313039200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bahatyrova, S.
Right arrow Articles by Olsen, J. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bahatyrova, S.
Right arrow Articles by Olsen, J. D.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Flexibility and Size Heterogeneity of the LH1 Light Harvesting Complex Revealed by Atomic Force Microscopy

FUNCTIONAL SIGNIFICANCE FOR BACTERIAL PHOTOSYNTHESIS*

Svetlana Bahatyrova{ddagger}, Raoul N. Frese{ddagger}§, Kees O. van der Werf{ddagger}, Cees Otto{ddagger}, C. Neil Hunter¶||, and John D. Olsen¶**

From the {ddagger}Biophysical Techniques Group, Department of Science and Technology, University of Twente, 7500 AE Enschede, The Netherlands and Robert Hill Institute for Photosynthesis Research, Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom

Previous electron microscopic studies of bacterial RCLH1 complexes demonstrated both circular and elliptical conformations of the LH1 ring, and this implied flexibility has been suggested to allow passage of quinol from the QB site of the RC to the quinone pool prior to reduction of the cytochrome bc1 complex. We have used atomic force microscopy to demonstrate that these are just two of many conformations for the LH1 ring, which displays large molecule-to-molecule variations, in terms of both shape and size. This atomic force microscope study has used a mutant lacking the reaction center complex, which normally sits within the LH1 ring providing a barrier to substantial changes in shape. This approach has revealed the inherent flexibility and lack of structural coherence of this complex in a reconstituted lipid bilayer at room temperature. Circular, elliptical, and even polygonal ring shapes as well as arcs and open rings have been observed for LH1; in contrast, no such variations in structure were observed for the LH2 complex under the same conditions. The basis for these differences between LH1 and LH2 is suggested to be the H-bonding patterns that stabilize binding of the bacteriochlorophylls to the LH polypeptides. The existence of open rings and arcs provides a direct visualization of the consequences of the relatively weak associations that govern the aggregation of the protomers ({alpha}1{beta}1Bchl2) comprising the LH1 complex. The demonstration that the linkage between adjacent protomer units is flexible and can even be uncoupled at room temperature in a detergent-free membrane bilayer provides a rationale for the dynamic separation of individual protomers, and we may now envisage experiments that seek to prove this active opening process.


Received for publication, December 1, 2003 , and in revised form, February 2, 2004.

* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains one figure and two tables.

§ Supported by the Netherlands Organization for Scientific Research.

|| Funded by the Biotechnology and Biological Sciences Research Council.

** Funded by the Biotechnology and Biological Sciences Research Council. To whom correspondence should be addressed. Tel.: 440-114-222-4240; Fax: 440-114-222-2711; E-mail: j.olsen{at}sheffield.ac.uk.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
Biophys. JHome page
D. E. Chandler, J. Hsin, C. B. Harrison, J. Gumbart, and K. Schulten
Intrinsic Curvature Properties of Photosynthetic Proteins in Chromatophores
Biophys. J., September 15, 2008; 95(6): 2822 - 2836.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
P. Qian, P. A. Bullough, and C. N. Hunter
Three-dimensional Reconstruction of a Membrane-bending Complex: THE RC-LH1-PufX CORE DIMER OF RHODOBACTER SPHAEROIDES
J. Biol. Chem., May 16, 2008; 283(20): 14002 - 14011.
[Abstract] [Full Text] [PDF]


Home page
Biophys. JHome page
J. Janusonis, L. Valkunas, D. Rutkauskas, and R. van Grondelle
Spectral Dynamics of Individual Bacterial Light-Harvesting Complexes: Alternative Disorder Model
Biophys. J., February 15, 2008; 94(4): 1348 - 1358.
[Abstract] [Full Text] [PDF]


Home page
Biophys. JHome page
R. N. Frese, J. C. Pamies, J. D. Olsen, S. Bahatyrova, C. D. van der Weij-de Wit, T. J. Aartsma, C. Otto, C. N. Hunter, D. Frenkel, and R. van Grondelle
Protein Shape and Crowding Drive Domain Formation and Curvature in Biological Membranes
Biophys. J., January 15, 2008; 94(2): 640 - 647.
[Abstract] [Full Text] [PDF]


Home page
Biophys. JHome page
T. Geyer
On the Effects of PufX on the Absorption Properties of the Light-Harvesting Complexes of Rhodobacter sphaeroides
Biophys. J., December 15, 2007; 93(12): 4374 - 4381.
[Abstract] [Full Text] [PDF]


Home page
Biophys. JHome page
D. Rutkauskas, J. Olsen, A. Gall, R. J. Cogdell, C. N. Hunter, and R. van Grondelle
Comparative Study of Spectral Flexibilities of Bacterial Light-Harvesting Complexes: Structural Implications
Biophys. J., April 1, 2006; 90(7): 2463 - 2474.
[Abstract] [Full Text] [PDF]


Home page
Biophys. JHome page
D. Rutkauskas, V. Novoderezhkin, A. Gall, J. Olsen, R. J. Cogdell, C. N. Hunter, and R. van Grondelle
Spectral Trends in the Fluorescence of Single Bacterial Light-Harvesting Complexes: Experiments and Modified Redfield Simulations
Biophys. J., April 1, 2006; 90(7): 2475 - 2485.
[Abstract] [Full Text] [PDF]


Home page
Biophys. JHome page
S. Georgakopoulou, R. van Grondelle, and G. van der Zwan
Circular Dichroism of Carotenoids in Bacterial Light-Harvesting Complexes: Experiments and Modeling
Biophys. J., November 1, 2004; 87(5): 3010 - 3022.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.