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J. Biol. Chem., Vol. 279, Issue 21, 22204-22208, May 21, 2004

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Progression and Specificity of Protein Oxidation in the Life Cycle of Arabidopsis thaliana^*

Elin Johansson, Olof Olsson, and Thomas Nyström

From the Department of Cell and Molecular Biology, Göteborg University, Box 462, 405 30 Göteborg, Sweden

Protein carbonylation is an irreversible oxidative process leading to a loss of function of the modified proteins, and in a variety of model systems, including worms, flies, and mammals, carbonyl levels gradually increase with age. In contrast, we report here that in Arabidopsis thaliana an initial increase in protein oxidation during the first 20 days of the life cycle of the plant is followed by a drastic reduction in protein carbonyls prior to bolting and flower development. Protein carbonylation prior to the transition to flowering targets specific proteins such as Hsp70, ATP synthases, the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), and proteins involved in light harvesting/energy transfer and the C2 oxidative photosynthetic carbon cycle. The precipitous fall in protein carbonyl levels is due to the specific reduction in the levels of oxidized proteins rather than an overall loss of chlorophyll and Rubisco associated with the senescence syndrome. The results are discussed in light of contemporary theories of aging in animals.

Received for publication, March 9, 2004

^* This work was supported by a grant from the Swedish Natural Science Research Council (to T. N.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dept. of Cell and Molecular Biology, Göteborg Univerity, Box 462, 40530 Göteborg, Sweden. Tel.: 46-31-7732582; Fax: 46-31-7732599; E-mail: Thomas.Nystrom{at}gmm.gu.se.

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