| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 279, Issue 21, 22522-22531, May 21, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Departments of Psychiatry and Cellular and Molecular Pharmacology, University of California, San Francisco, California 94143-2140
Many signaling receptors require covalent modification by ubiquitin for agonist-induced down-regulation via endocytic trafficking to lysosomes, a process that is mediated by a conserved set of endosome-associating proteins also required for vacuolar protein-sorting (VPS) in yeast. The delta opioid receptor (DOR) is a G protein-coupled receptor that can undergo agonist-induced proteolysis via endocytic trafficking to lysosomes but does not require covalent modification by ubiquitin to do so. This raises the question of whether lysosomal down-regulation of this "ubiquitination-independent" GPCR is mediated by a completely distinct biochemical mechanism or if similar VPS machinery is involved. Agonist-induced proteolysis of DOR was significantly inhibited by dominant negative mutant versions of Vps4/Skd1, an AAA-family ATPase required for a late step in lysosomal sorting of ubiquitinated membrane cargo. Furthermore, overexpression and interfering RNA-mediated knockdown indicated that lysosomal trafficking of opioid receptors is also dependent on Hrs, a VPS protein that mediates an early step in lysosomal sorting of ubiquitinated cargo. However, interfering RNA-mediated knockdown of Tsg101, a VPS protein that is essential for an intermediate step of the conserved lysosomal sorting mechanism, did not detectably affect agonist-induced proteolysis of DOR in the same cells in which (ubiquitination-dependent) lysosomal trafficking of epidermal growth factor receptors was clearly inhibited. These results indicate that opioid receptors, despite their ability to undergo efficient agonist-induced trafficking to lysosomes in the absence of covalent modification by ubiquitin, utilize some (Vps4 and Hrs) but perhaps not all (Tsg101) of the VPS machinery required for lysosomal sorting of ubiquitinated membrane cargo.
Received for publication, October 8, 2003 , and in revised form, March 10, 2004.
* This work was supported in part by a research grant from the National Institutes of Health/National Institute on Drug Abuse (to M. v. Z.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Both authors contributed equally to this work.
Supported by a Postdoctoral Research Fellowship from the American Heart Association. To whom correspondence should be addressed: N-216 Genentech Hall, University of California, San Francisco, 600 16th Street, San Francisco, CA 94143-2140. Tel.: 415-476-7871; Fax: 415-504-0169; E-mail: jhislop{at}itsa.ucsf.edu.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. S. Spellman, K. Deinhardt, C. C. Darie, M. V. Chao, and T. A. Neubert Stable Isotopic Labeling by Amino Acids in Cultured Primary Neurons: Application to Brain-derived Neurotrophic Factor-dependent Phosphotyrosine-associated Signaling Mol. Cell. Proteomics, June 1, 2008; 7(6): 1067 - 1076. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yamashita, K. Kojima, T. Tsukahara, H. Agawa, K. Yamada, Y. Amano, N. Kurotori, N. Tanaka, K. Sugamura, and T. Takeshita Ubiquitin-independent binding of Hrs mediates endosomal sorting of the interleukin-2 receptor {beta}-chain J. Cell Sci., May 15, 2008; 121(10): 1727 - 1738. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-G. Li, D. S. Haines, and L.-Y. Liu-Chen Agonist-Promoted Lys63-Linked Polyubiquitination of the Human {kappa}-Opioid Receptor Is Involved in Receptor Down-Regulation Mol. Pharmacol., April 1, 2008; 73(4): 1319 - 1330. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Taylor, P. I. Hanson, and M. Kielian Ubiquitin Depletion and Dominant-Negative VPS4 Inhibit Rhabdovirus Budding without Affecting Alphavirus Budding J. Virol., December 15, 2007; 81(24): 13631 - 13639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Hasdemir, N. W. Bunnett, and G. S. Cottrell Hepatocyte Growth Factor-regulated Tyrosine Kinase Substrate (HRS) Mediates Post-endocytic Trafficking of Protease-activated Receptor 2 and Calcitonin Receptor-like Receptor J. Biol. Chem., October 5, 2007; 282(40): 29646 - 29657. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Huang, F. Reggiori, and D. J. Klionsky The Transmembrane Domain of Acid Trehalase Mediates Ubiquitin-independent Multivesicular Body Pathway Sorting Mol. Biol. Cell, July 1, 2007; 18(7): 2511 - 2524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. De Domenico, D. M. Ward, C. Langelier, M. B. Vaughn, E. Nemeth, W. I. Sundquist, T. Ganz, G. Musci, and J. Kaplan The Molecular Mechanism of Hepcidin-mediated Ferroportin Down-Regulation Mol. Biol. Cell, July 1, 2007; 18(7): 2569 - 2578. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Shenoy Seven-Transmembrane Receptors and Ubiquitination Circ. Res., April 27, 2007; 100(8): 1142 - 1154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Cottrell, B. Padilla, S. Pikios, D. Roosterman, M. Steinhoff, E. F. Grady, and N. W. Bunnett Post-endocytic Sorting of Calcitonin Receptor-like Receptor and Receptor Activity-modifying Protein 1 J. Biol. Chem., April 20, 2007; 282(16): 12260 - 12271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Hanyaloglu and M. von Zastrow A Novel Sorting Sequence in the beta2-Adrenergic Receptor Switches Recycling from Default to the Hrs-dependent Mechanism J. Biol. Chem., February 2, 2007; 282(5): 3095 - 3104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. McNatt, I. McKittrick, M. West, and G. Odorizzi Direct Binding to Rsp5 Mediates Ubiquitin-independent Sorting of Sna3 via the Multivesicular Body Pathway Mol. Biol. Cell, February 1, 2007; 18(2): 697 - 706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Oestreich, M. Aboian, J. Lee, I. Azmi, J. Payne, R. Issaka, B. A. Davies, and D. J. Katzmann Characterization of Multiple Multivesicular Body Sorting Determinants within Sna3: A Role for the Ubiquitin Ligase Rsp5 Mol. Biol. Cell, February 1, 2007; 18(2): 707 - 720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kirk, L.-S. Chin, and L. Li GRIF1 binds Hrs and is a new regulator of endosomal trafficking J. Cell Sci., November 15, 2006; 119(22): 4689 - 4701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Langelier, U. K. von Schwedler, R. D. Fisher, I. De Domenico, P. L. White, C. P. Hill, J. Kaplan, D. Ward, and W. I. Sundquist Human ESCRT-II Complex and Its Role in Human Immunodeficiency Virus Type 1 Release J. Virol., October 1, 2006; 80(19): 9465 - 9480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Cottrell, B. Padilla, S. Pikios, D. Roosterman, M. Steinhoff, D. Gehringer, E. F. Grady, and N. W. Bunnett Ubiquitin-dependent Down-regulation of the Neurokinin-1 Receptor J. Biol. Chem., September 22, 2006; 281(38): 27773 - 27783. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Drake, S. K. Shenoy, and R. J. Lefkowitz Trafficking of G Protein-Coupled Receptors Circ. Res., September 15, 2006; 99(6): 570 - 582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Razi and C. E. Futter Distinct Roles for Tsg101 and Hrs in Multivesicular Body Formation and Inward Vesiculation Mol. Biol. Cell, August 1, 2006; 17(8): 3469 - 3483. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. van Niel, I. Porto-Carreiro, S. Simoes, and G. Raposo Exosomes: a common pathway for a specialized function. J. Biochem., July 1, 2006; 140(1): 13 - 21. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Jou, C.-P. Chiang, G.-Y. Jauh, and H. E. Yen Functional Characterization of Ice Plant SKD1, an AAA-Type ATPase Associated with the Endoplasmic Reticulum-Golgi Network, and Its Role in Adaptation to Salt Stress Plant Physiology, May 1, 2006; 141(1): 135 - 146. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Staub and D. Rotin Role of Ubiquitylation in Cellular Membrane Transport Physiol Rev, April 1, 2006; 86(2): 669 - 707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gullapalli, B. L. Wolfe, C. T. Griffin, T. Magnuson, and J. Trejo An Essential Role for SNX1 in Lysosomal Sorting of Protease-activated Receptor-1: Evidence for Retromer-, Hrs-, and Tsg101-independent Functions of Sorting Nexins Mol. Biol. Cell, March 1, 2006; 17(3): 1228 - 1238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Salas-Cortes, F. Ye, D. Tenza, C. Wilhelm, A. Theos, D. Louvard, G. Raposo, and E. Coudrier Myosin Ib modulates the morphology and the protein transport within multi-vesicular sorting endosomes J. Cell Sci., October 15, 2005; 118(20): 4823 - 4832. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Bartlett, J. Enquist, F. W. Hopf, J. H. Lee, F. Gladher, V. Kharazia, M. Waldhoer, W. S. Mailliard, R. Armstrong, A. Bonci, et al. Dopamine responsiveness is regulated by targeted sorting of D2 receptors PNAS, August 9, 2005; 102(32): 11521 - 11526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-C. Ma, H. Qian, F. Ghassemi, P. Zhao, and Y. Xia Oxygen-sensitive {delta}-Opioid Receptor-regulated Survival and Death Signals: NOVEL INSIGHTS INTO NEURONAL PRECONDITIONING AND PROTECTION J. Biol. Chem., April 22, 2005; 280(16): 16208 - 16218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Zheng, C. Lavoie, T.-D. Tang, P. Ma, T. Meerloo, A. Beas, and M. G. Farquhar Regulation of Epidermal Growth Factor Receptor Degradation by Heterotrimeric G{alpha}s Protein Mol. Biol. Cell, December 1, 2004; 15(12): 5538 - 5550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Stuchell, J. E. Garrus, B. Muller, K. M. Stray, S. Ghaffarian, R. McKinnon, H.-G. Krausslich, S. G. Morham, and W. I. Sundquist The Human Endosomal Sorting Complex Required for Transport (ESCRT-I) and Its Role in HIV-1 Budding J. Biol. Chem., August 20, 2004; 279(34): 36059 - 36071. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
