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J. Biol. Chem., Vol. 279, Issue 22, 23447-23452, May 28, 2004

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Crystal Structure of Yeast Allantoicase Reveals a Repeated Jelly Roll Motif^*

Nicolas Leulliot, Sophie Quevillon-Cheruel, Isabelle Sorel, Marc Graille, Philippe Meyer, Dominique Liger, Karine Blondeau¶, Joël Janin, and Herman van Tilbeurgh||

From the Institut de Biochimie et de Biophysique Moléculaire et Cellulaire (CNRS-Unité Mixte de Recherche 8619), Université Paris-Sud, Bâtiment 430, 91405 Orsay, Laboratoire d'Enzymologie et Biochimie Structurales (CNRS-Unité Propre de Recherche 9063), Bâtiment 34, 1 Avenue de la Terrasse, 91198 Gif sur Yvette, and ^¶Institut de Génétique et Microbiologie (CNRS-Unité Mixte de Recherche 8621), Université Paris-Sud, Bâtiment 360, 91405 Orsay, France

Allantoicase (EC 3.5.3.4) catalyzes the conversion of allantoate into ureidoglycolate and urea, one of the final steps in the degradation of purines to urea. The mechanism of most enzymes involved in this pathway, which has been known for a long time, is unknown. In this paper we describe the three-dimensional crystal structure of the yeast allantoicase determined at a resolution of 2.6 Å by single anomalous diffraction. This constitutes the first structure for an enzyme of this pathway. The structure reveals a repeated jelly roll -sheet motif, also present in proteins of unrelated biochemical function. Allantoicase has a hexameric arrangement in the crystal (dimer of trimers). Analysis of the protein sequence against the structural data reveals the presence of two totally conserved surface patches, one on each jelly roll motif. The hexameric packing concentrates these patches into conserved pockets that probably constitute the active site.

Received for publication, February 6, 2004 , and in revised form, March 4, 2004.

The atomic coordinates and structure factors (code 1SG3) have been deposited in the Protein Data Bank, Research Collaboratory for Structural Bioinformatics, Rutgers University, New Brunswick, NJ (http://www.rcsb.org/).

^* This work was supported by grants from the Ministère de la Recherche et de la Technologie (Programme Génopoles) and the Association pour la Recherche contre le Cancer (to M. G.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^|| To whom correspondence should be addressed. Tel.: 33-1-69-82-34-91; Fax: 33-1-69-82-31-29; E-mail: herman{at}lebs.cnrs-gif.fr.

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