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J. Biol. Chem., Vol. 279, Issue 22, 23782-23789, May 28, 2004

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The Interactions of Allium sativum Leaf Agglutinin with a Chaperonin Group of Unique Receptor Protein Isolated from a Bacterial Endosymbiont of the Mustard Aphid^*

Santanu Banerjee, Daniel Hess¶, Pralay Majumder||, Debjani Roy**, and Sampa Das

From the Plant Molecular and Cellular Genetics, Bose Institute, P-1/12, C.I.T. Scheme, VII-M, Calcutta 700054, India, the ^¶Protein and Peptide Analytics Laboratory, Friedrich Miescher Institut for Biomedical Research, P.O. Box 2543, CH-4002 Basel, Switzerland, and ^**Bioinformatics Centre, Bose Institute, P1/12, C.I.T. Scheme, VII-M, Calcutta 700054, India

The homopteran sucking insect, Lipaphis erysimi (mustard aphid) causes severe damage to various crops. This pest not only affects plants by sucking on the phloem, but it also transmits single-stranded RNA luteoviruses while feeding, which cause disease and damage in the crop. The mannose-binding Allium sativum (garlic) leaf lectin has been found to be a potent control agent of L. erysimi. The lectin receptor protein isolated from brush border membrane vesicle of insect gut was purified to determine the mechanism of lectin binding to the gut. Purified receptor was identified as an endosymbiotic chaperonin, symbionin, using liquid chromatography-tandem mass spectrometry. Symbionin from endosymbionts of other aphid species have been reported to play a significant role in virus transmission by binding to the read-through domain of the viral coat protein. To understand the molecular interactions of the said lectin and this unique symbionin molecule, the model structures of both molecules were generated using the Modeller program. The interaction was confirmed through docking of the two molecules forming a complex. A surface accessibility test of these molecules demonstrated a significant reduction in the accessibility of the complex molecule compared with that of the free symbionin molecule. This reduction in surface accessibility may have an effect on other molecular interactive processes, including "symbionin virion recognition", which is essential for such symbionin-mediated virus transmission. Thus, garlic leaf lectin provides an important component of a crop management program by controlling, on one hand, aphid attack and on the other hand, symbionin-mediated luteovirus transmission.

Received for publication, February 9, 2004 , and in revised form, March 16, 2004.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Provided a Fellowship and infrastructural facilities by Bose Institute, Calcutta, India.

^|| Provided a Fellowship by the Council for Scientific and Industrial Research (CSIR).

Funded by the Department of Atomic Energy, Government of India. To whom correspondence should be addressed. Tel.: 91-033-23379544; Fax: 91-033-23343886; E-mail: sampa{at}bic.boseinst.ernet.in.

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