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J. Biol. Chem., Vol. 279, Issue 23, 24485-24492, June 4, 2004

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Biochemical Differences in the T Cell Receptor·CD3 Surface Complex between CD8⁺ and CD4⁺ Human Mature T Lymphocytes^*

David A. Zapata, Wolfgang W. A. Schamel¶, Pilar S. Torres, Balbino Alarcón¶, Nineth E. Rossi||, María N. Navarro¶, María L. Toribio¶, and José R. Regueiro**

From the Inmunología, Facultad de Medicina, Universidad Complutense, 28040 Madrid, Spain and the ^¶Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain

We have reported the existence of biochemical and conformational differences in the T cell receptor (TCR) complex between CD4⁺ and CD8⁺ CD3-deficient (^-) mature T cells. In the present study, we have furthered our understanding and extended the observations to primary T lymphocytes from normal (⁺) individuals. Surface TCR·CD3 components from CD4^{+ -} T cells, other than CD3, were detectable and similar in size to CD4^{+ +} controls. Their native TCR·CD3 complex was also similar to CD4^{+ +} controls, except for an ()₂₂ instead of an ₂ stoichiometry. In contrast, the surface TCR, TCR, and CD3 chains of CD8^{+ -} T cells did not possess their usual sizes. Using confocal immunofluorescence, TCR was hardly detectable in CD8^{+ -} T cells. Blue native gels (BN-PAGE) demonstrated the existence of a heterogeneous population of TCR·CD3 in these cells. Using primary peripheral blood T lymphocytes from normal (⁺) donors, we performed a broad epitopic scan. In contrast to all other TCR·CD3-specific monoclonal antibodies, RW2-8C8 stained CD8⁺ better than it did CD4⁺ T cells, and the difference was dependent on glycosylation of the TCR·CD3 complex but independent of T cell activation or differentiation. RW2-8C8 staining of CD8⁺ T cells was shown to be more dependent on lipid raft integrity than that of CD4⁺ T cells. Finally, immunoprecipitation studies on purified primary CD4⁺ and CD8⁺ T cells revealed the existence of TCR glycosylation differences between the two. Collectively, these results are consistent with the existence of conformational or topological lineage-specific differences in the TCR·CD3 from CD4⁺ and CD8⁺ wild type T cells. The differences may be relevant for cis interactions during antigen recognition and signal transduction.

Received for publication, October 20, 2003 , and in revised form, February 27, 2004.

^* This work was supported by grants from the Ministerio de Ciencia y Tecnología (BMC2002-3247) and the Comunidad Autónoma de Madrid (21/01) (to J. R. R.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Supported by Comunidad Autónoma de Madrid.

^|| Supported by the Universidad de Los Andes (Venezuela).

^** To whom correspondence should be addressed. Tel.: 34-91-394-1642; Fax: 34-91-394-1641; E-mail: regueiro{at}med.ucm.es.

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