HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 23, 24640-24648, June 4, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/23/24640    most recent M400662200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Loh, E. Articles by Hong, W. Search for Related Content PubMed PubMed Citation Articles by Loh, E. Articles by Hong, W. Social Bookmarking                      What's this?

The Binary Interacting Network of the Conserved Oligomeric Golgi Tethering Complex^*

Eva Loh and Wanjin Hong

From the Membrane Biology Laboratory, Institute of Molecular and Cell Biology, 30 Medical Dr., Singapore 117609, Singapore

Several recent studies have revealed the existence of a conserved oligomeric Golgi (COG) complex consisting of several novel proteins as well as known Golgi proteins that were identified by independent approaches. The mammalian COG complex contains eight subunits: COG1/LdlBp, COG2/LdlCp, COG3/Sec34, COG4/Cod1, COG5/GTC-90/Cod4, COG6/Cod2, COG7, and COG8/Dor1. COG1, COG2, and COG7 seem structurally unique to mammalian cells, whereas the other five subunits are structurally conserved in yeast, which also contains three other unique proteins (COG1/Sec36p/Cod3p, COG2/Sec35p, and COG7/Cod5p). We report here the network of intermolecular interactions of the COG complex, revealed by in vitro translation and co-immunoprecipitation approaches. Our results suggest that COG4 serves as a core component of the complex by interacting directly with COG1, COG2, COG5, and COG7. COG3 is incorporated by its direct interaction with COG1 and COG2, whereas COG6 and COG8 do not interact with any individual subunit. Incorporation of COG6 into the complex depends on the concerted interaction of both COG5 and COG7, whereas optimal incorporation of COG8 depends on the concerted interaction of COG5, COG6, and COG7. Because COG4 (together with COG1, COG2, and COG3) is among the four essential genes of the COG complex in yeast, this molecular network highlights the structural basis for a crucial role of COG4 in the assembly/function of the complex. A model for the assembly of the COG complex is presented.

Received for publication, January 20, 2004 , and in revised form, February 26, 2004.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: 65-6778-6827; Fax: 65-6779-1117; E-mail: mcbhwj{at}imcb.a-star.edu.sg.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. F. Cavanaugh, X. Chen, B. C. Richardson, D. Ungar, I. Pelczer, J. Rizo, and F. M. Hughson Structural Analysis of Conserved Oligomeric Golgi Complex Subunit 2 J. Biol. Chem., August 10, 2007; 282(32): 23418 - 23426. [Abstract] [Full Text] [PDF]

				F. Foulquier, D. Ungar, E. Reynders, R. Zeevaert, P. Mills, M. T. Garcia-Silva, P. Briones, B. Winchester, W. Morelle, M. Krieger, et al. A new inborn error of glycosylation due to a Cog8 deficiency reveals a critical role for the Cog1-Cog8 interaction in COG complex formation Hum. Mol. Genet., April 1, 2007; 16(7): 717 - 730. [Abstract] [Full Text] [PDF]

				S. Wopereis, D. J. Lefeber, E. Morava, and R. A. Wevers Mechanisms in Protein O-Glycan Biosynthesis and Clinical and Molecular Aspects of Protein O-Glycan Biosynthesis Defects: A Review Clin. Chem., April 1, 2006; 52(4): 574 - 600. [Abstract] [Full Text] [PDF]

				F. Foulquier, E. Vasile, E. Schollen, N. Callewaert, T. Raemaekers, D. Quelhas, J. Jaeken, P. Mills, B. Winchester, M. Krieger, et al. Conserved oligomeric Golgi complex subunit 1 deficiency reveals a previously uncharacterized congenital disorder of glycosylation type II. PNAS, March 7, 2006; 103(10): 3764 - 3769. [Abstract] [Full Text] [PDF]

				Y. Kubota, M. Sano, S. Goda, N. Suzuki, and K. Nishiwaki The conserved oligomeric Golgi complex acts in organ morphogenesis via glycosylation of an ADAM protease in C. elegans Development, January 15, 2006; 133(2): 263 - 273. [Abstract] [Full Text] [PDF]

				E. Sztul and V. Lupashin Role of tethering factors in secretory membrane traffic Am J Physiol Cell Physiol, January 1, 2006; 290(1): C11 - C26. [Abstract] [Full Text] [PDF]

				D. Ungar, T. Oka, E. Vasile, M. Krieger, and F. M. Hughson Subunit Architecture of the Conserved Oligomeric Golgi Complex J. Biol. Chem., September 23, 2005; 280(38): 32729 - 32735. [Abstract] [Full Text] [PDF]

				T. Oka, E. Vasile, M. Penman, C. D. Novina, D. M. Dykxhoorn, D. Ungar, F. M. Hughson, and M. Krieger Genetic Analysis of the Subunit Organization and Function of the Conserved Oligomeric Golgi (COG) Complex: STUDIES OF COG5- AND COG7-DEFICIENT MAMMALIAN CELLS J. Biol. Chem., September 23, 2005; 280(38): 32736 - 32745. [Abstract] [Full Text] [PDF]

				P. Fotso, Y. Koryakina, O. Pavliv, A. B. Tsiomenko, and V. V. Lupashin Cog1p Plays a Central Role in the Organization of the Yeast Conserved Oligomeric Golgi Complex J. Biol. Chem., July 29, 2005; 280(30): 27613 - 27623. [Abstract] [Full Text] [PDF]

				S. N. Zolov and V. V. Lupashin Cog3p depletion blocks vesicle-mediated Golgi retrograde trafficking in HeLa cells J. Cell Biol., February 28, 2005; 168(5): 747 - 759. [Abstract] [Full Text] [PDF]

				P. Bruinsma, R. G. Spelbrink, and S. F. Nothwehr Retrograde Transport of the Mannosyltransferase Och1p to the Early Golgi Requires a Component of the COG Transport Complex J. Biol. Chem., September 17, 2004; 279(38): 39814 - 39823. [Abstract] [Full Text] [PDF]