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J. Biol. Chem., Vol. 279, Issue 24, 25017-25023, June 11, 2004

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F-actin-dependent Insolubility of Chromatin-modifying Components^*

Christi Andrin and Michael J. Hendzel

From the Department of Oncology, University of Alberta, Edmonton, Alberta T6G 1Z2, Canada

Many complexes involved in chromatin modification are difficult to isolate and commonly found associated with nuclear matrix preparations. In this study, we examine the elution properties of chromatin-modifying components under different extraction conditions. We find that most, but not all, histone acetyltransferases and histone deacetylases predominantly partition with the nuclear pellet during intermediate salt extraction. In attempts to identify a biological basis for the observed insolubility, we demonstrate that depolymerizing cellular actin, but not cellular tubulin, mobilizes a significant proportion of the insoluble pool into the intermediate salt-soluble nuclear extract. The disruption of cellular F-actin releases a specific subset of high molecular weight, active, nuclear histone deacetylase complexes that are not found under normal conditions. This study demonstrates that actin polymerization, a physiologically relevant process, is responsible for the observed insolubility of these components during nuclear extract preparation and establishes a simple strategy for isolating subsets of chromatin-modifying complexes that are otherwise depleted or absent under the same extraction conditions.

Received for publication, February 18, 2004 , and in revised form, April 8, 2004.

^* This work was supported by an operating grant from the Canadian Institutes of Health Research. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

A scholar of the Alberta Heritage Foundation for Medical Research and a Canadian Institutes of Health Research New Investigator. To whom correspondence should be addressed: Rm. 3332, 11560 University Ave., Edmonton, Alberta T6G 1Z2, Canada. Tel.: 780-432-8439; Fax: 780-432-8892; E-mail: michaelh{at}cancerboard.ab.ca.

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