HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 24, 25381-25389, June 11, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/24/25381    most recent M403121200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Magdinier, F. Articles by Felsenfeld, G. Search for Related Content PubMed PubMed Citation Articles by Magdinier, F. Articles by Felsenfeld, G. Social Bookmarking                      What's this?

Both CTCF-dependent and -independent Insulators Are Found between the Mouse T Cell Receptor and Dad1 Genes^*

Frédérique Magdinier, Timur M. Yusufzai, and Gary Felsenfeld

From the Laboratory of Molecular Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892-0504

The T cell rearrangement of the T cell receptor (TCR) genes TCR and is specifically regulated by a complex interplay between enhancer elements and chromatin structure. The enhancer is active in T cells and drives TCR recombination in collaboration with a locus control region-like element located downstream of the C gene on mouse chromosome 14. Twelve kb further down-stream lies another gene, Dad1, with a program of expression different from that of TCR. The 6-kb locus control region element lying between them contains multiple regulatory sites with a variety of roles in regulating the two genes. Previous evidence has indicated that among these there are widely distributed regions with enhancer blocking (insulating) activity. We have shown in this report that one of these sites, not previously examined, strongly binds the insulator protein CCTC-binding factor (CTCF) in vitro and in vivo and can function in an enhancer blocking assay. However, other regions within the 6-kb element that also can block enhancers clearly do not harbor CTCF sites and thus must reflect the presence of a previously undetected and distinct vertebrate insulator activity.

Received for publication, March 19, 2004 , and in revised form, April 12, 2004.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Present address: Laboratoire de Biologie Moléculaire de la cellule, CNRS Unite Mixte de Recherche 5161, Ecole Normale Supérieure, 46 Allée d'Italie, 69364 Lyon cedex 07, France.

To whom correspondence should be addressed. Tel.: 301-496-4173; Fax: 301-496-0201; E-mail: gary.felsenfeld{at}nih.gov.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. Ribeiro de Almeida, H. Heath, S. Krpic, G. M. Dingjan, J. P. van Hamburg, I. Bergen, S. van de Nobelen, F. Sleutels, F. Grosveld, N. Galjart, et al. Critical Role for the Transcription Regulator CCCTC-Binding Factor in the Control of Th2 Cytokine Expression J. Immunol., January 15, 2009; 182(2): 999 - 1010. [Abstract] [Full Text] [PDF]

				J. Murai, D. Ikegami, M. Okamoto, H. Yoshikawa, and N. Tsumaki Insulation of the Ubiquitous Rxrb Promoter from the Cartilage-specific Adjacent Gene, Col11a2 J. Biol. Chem., October 10, 2008; 283(41): 27677 - 27687. [Abstract] [Full Text] [PDF]

				J. Gomos-Klein, F. Harrow, J. Alarcon, and B. D. Ortiz CTCF-Independent, but Not CTCF-Dependent, Elements Significantly Contribute to TCR-{alpha} Locus Control Region Activity J. Immunol., July 15, 2007; 179(2): 1088 - 1095. [Abstract] [Full Text] [PDF]

				P. Majumder, J. A. Gomez, and J. M. Boss The Human Major Histocompatibility Complex Class II HLA-DRB1 and HLA-DQA1 Genes Are Separated by a CTCF-binding Enhancer-blocking Element J. Biol. Chem., July 7, 2006; 281(27): 18435 - 18443. [Abstract] [Full Text] [PDF]

				A. G. West and P. Fraser Remote control of gene transcription Hum. Mol. Genet., April 15, 2005; 14(suppl_1): R101 - R111. [Abstract] [Full Text] [PDF]

				G. FELSENFELD, B. BURGESS-BEUSSE, C. FARRELL, M. GASZNER, R. GHIRLANDO, S. HUANG, C. JIN, M. LITT, F. MAGDINIER, V. MUTSKOV, et al. Chromatin Boundaries and Chromatin Domains Cold Spring Harb Symp Quant Biol, January 1, 2004; 69(0): 245 - 250. [Abstract] [PDF]