HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 25, 26098-26104, June 18, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/25/26098    most recent M402807200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Smith, A. P. Articles by Goldsbrough, P. B. Search for Related Content PubMed PubMed Citation Articles by Smith, A. P. Articles by Goldsbrough, P. B. Social Bookmarking                      What's this?

Proteomic Analysis of Arabidopsis Glutathione S-transferases from Benoxacor- and Copper-treated Seedlings^*

Aaron P. Smith¶, Ben P. DeRidder||, Woei-Jiun Guo, Erin H. Seeley**, Fred E. Regnier**, and Peter B. Goldsbrough

From the Departments of Horticulture and Landscape Architecture and ^**Chemistry, Purdue University, West Lafayette, Indiana 47907-2010

Glutathione S-transferases (GSTs) are involved in many stress responses in plants, for example, participating in the detoxification of xenobiotics and limiting oxidative damage. Studies examining the regulation of this gene family in diverse plant species have focused primarily on RNA expression. A proteomics method was developed to identify GSTs expressed in Arabidopsis seedlings and to determine how the abundance of these proteins changed in response to copper, a promoter of oxidative stress, and benoxacor, a herbicide safener. Eight GSTs were identified in seedlings grown under control conditions, and only one, AtGSTU19, was induced by benoxacor. In contrast, four GSTs, AtGSTF2, AtGSTF6, AtGSTF7, and AtGSTU19, were significantly more abundant in copper-treated seedlings. The different responses to these treatments may reflect the potential for copper to affect many more aspects of plant growth and physiology compared with a herbicide safener. Differences between RNA and protein expression of GSTs indicate that both transcriptional and translational mechanisms are involved in regulation of GSTs under these conditions.

Received for publication, March 12, 2004

^* This research was supported in part by grants from the National Institutes of Health and the National Research Initiative Program, U. S. Department of Agriculture. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Both authors contributed equally to this work.

^¶ Present address: Dept. of Genetics, The University of Georgia, Athens, GA 30602.

^|| Present address: U. S. Dept. of Agriculture, Agricultural Research Service, Pacific West Area, 4135 E. Broadway, Phoenix, AZ 85040.

To whom correspondence should be addressed. Tel.: 765-494-1334; Fax: 765-494-0391; E-mail: goldsbrough{at}purdue.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Brazier-Hicks, K. M. Evans, O. D. Cunningham, D. R. W. Hodgson, P. G. Steel, and R. Edwards Catabolism of Glutathione Conjugates in Arabidopsis thaliana: ROLE IN METABOLIC REACTIVATION OF THE HERBICIDE SAFENER FENCLORIM J. Biol. Chem., July 25, 2008; 283(30): 21102 - 21112. [Abstract] [Full Text] [PDF]

				W.-J. Guo, M. Meetam, and P. B. Goldsbrough Examining the Specific Contributions of Individual Arabidopsis Metallothioneins to Copper Distribution and Metal Tolerance Plant Physiology, April 1, 2008; 146(4): 1697 - 1706. [Abstract] [Full Text] [PDF]

				R. Hebeler, S. Oeljeklaus, K. A. Reidegeld, M. Eisenacher, C. Stephan, B. Sitek, K. Stuhler, H. E. Meyer, M. J. G. Sturre, P. P. Dijkwel, et al. Study of Early Leaf Senescence in Arabidopsis thaliana by Quantitative Proteomics Using Reciprocal 14N/15N Labeling and Difference Gel Electrophoresis Mol. Cell. Proteomics, January 1, 2008; 7(1): 108 - 120. [Abstract] [Full Text] [PDF]

				U. Roth, E. von Roepenack-Lahaye, and S. Clemens Proteome changes in Arabidopsis thaliana roots upon exposure to Cd2+ J. Exp. Bot., December 1, 2006; 57(15): 4003 - 4013. [Abstract] [Full Text] [PDF]

				B. P. DeRidder and P. B. Goldsbrough Organ-Specific Expression of Glutathione S-Transferases and the Efficacy of Herbicide Safeners in Arabidopsis Plant Physiology, January 1, 2006; 140(1): 167 - 175. [Abstract] [Full Text] [PDF]

				A. Gruhler, W. X. Schulze, R. Matthiesen, M. Mann, and O. N. Jensen Stable Isotope Labeling of Arabidopsis thaliana Cells and Quantitative Proteomics by Mass Spectrometry Mol. Cell. Proteomics, November 1, 2005; 4(11): 1697 - 1709. [Abstract] [Full Text] [PDF]