HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 26, 27116-27123, June 25, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/26/27116    most recent M403818200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kent, N. A. Articles by Mellor, J. Search for Related Content PubMed PubMed Citation Articles by Kent, N. A. Articles by Mellor, J. Social Bookmarking                      What's this?

Cbf1p Is Required for Chromatin Remodeling at Promoter-proximal CACGTG Motifs in Yeast^*

Nicholas A. Kent¶, Sybille M. Eibert||, and Jane Mellor

From the Genetics Unit and Microbiology Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom

Cbf1p is a basic-helix-loop-helix-zipper protein of Saccharomyces cerevisiae required for the function of centromeres and MET gene promoters, where it binds DNA via the consensus core motif CACRTG (R = A or G). At MET genes Cbf1p appears to function in both activator recruitment and chromatin-remodeling. Cbf1p has been implicated in the regulation of other genes, and CACRTG motifs are common in potential gene regulatory DNA. A recent genome-wide location analysis showed that the majority of intergenic CACGTG palindromes are bound by Cbf1p. Here we tested whether all potential Cbf1p binding motifs in the yeast genome are likely to be bound by Cbf1p using chromatin immunoprecipitation. We also tested which of the motifs are actually functional by assaying for Cbf1p-dependent chromatin remodeling. We show that Cbf1p binding and activity is restricted to palindromic CACGTG motifs in promoter-proximal regions. Cbf1p does not function through CACGTG motifs that occur in promoter-distal locations within coding regions nor where CACATG motifs occur alone except at centromeres. Cbf1p can be made to function at promoter-distal CACGTG motifs by overexpression, suggesting that the concentration of Cbf1p is normally limiting for binding and is biased to gene regulatory DNA by interactions with other factors. We conclude that Cbf1p is required for normal nucleosome positioning wherever the CACGTG motif occurs in gene regulatory DNA. Cbf1p has been shown to interact with the chromatin-remodeling ATPase Isw1p. Here we show that recruitment of Isw1p by Cbf1p is likely to be general but that Isw1p is only partially required for Cbf1p-dependent chromatin structures.

Received for publication, April 6, 2004 , and in revised form, April 23, 2004.

^* This work was supported by Wellcome Trust and Biotechnology and Biological Sciences Research Council, Swindon, United Kingdom grants (to J. M.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^|| Current address: Institut für Immunologie, Ruprecht-Karls-Universität, Im Neuenheimer Feld 305, 69120 Heidelberg, Germany.

^¶ To whom correspondence should be addressed. Tel.: 44-1-865-275325; Fax: 44-1-865-275318; E-mail: nicholas.kent{at}bioch.ox.ac.uk.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J.-N. Park, M. J. Sohn, D.-B. Oh, O. Kwon, S. K. Rhee, C.-G. Hur, S. Y. Lee, G. Gellissen, and H. A. Kang Identification of the Cadmium-Inducible Hansenula polymorpha SEO1 Gene Promoter by Transcriptome Analysis and Its Application to Whole-Cell Heavy-Metal Detection Systems Appl. Envir. Microbiol., October 1, 2007; 73(19): 5990 - 6000. [Abstract] [Full Text] [PDF]

				M. Chen and J. M. Lopes Multiple Basic Helix-Loop-Helix Proteins Regulate Expression of the ENO1 Gene of Saccharomyces cerevisiae Eukaryot. Cell, May 1, 2007; 6(5): 786 - 796. [Abstract] [Full Text] [PDF]

				D. B. Murray, M. Beckmann, and H. Kitano Regulation of yeast oscillatory dynamics PNAS, February 13, 2007; 104(7): 2241 - 2246. [Abstract] [Full Text] [PDF]

				S. J. Maerkl and S. R. Quake A Systems Approach to Measuring the Binding Energy Landscapes of Transcription Factors Science, January 12, 2007; 315(5809): 233 - 237. [Abstract] [Full Text] [PDF]

				L.-C. Lai, A. L. Kosorukoff, P. V. Burke, and K. E. Kwast Metabolic-State-Dependent Remodeling of the Transcriptome in Response to Anoxia and Subsequent Reoxygenation in Saccharomyces cerevisiae. Eukaryot. Cell, September 1, 2006; 5(9): 1468 - 1489. [Abstract] [Full Text] [PDF]

				A. Menant, P. Baudouin-Cornu, C. Peyraud, M. Tyers, and D. Thomas Determinants of the Ubiquitin-mediated Degradation of the Met4 Transcription Factor J. Biol. Chem., April 28, 2006; 281(17): 11744 - 11754. [Abstract] [Full Text] [PDF]