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J. Biol. Chem., Vol. 279, Issue 26, 27790-27798, June 25, 2004

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Mirk/dyrk1B Kinase Destabilizes Cyclin D1 by Phosphorylation at Threonine 288^*

Yonglong Zou, Daina Z. Ewton, Xiaobing Deng, Stephen E. Mercer, and Eileen Friedman

From the Department of Pathology, Upstate Medical University, Syracuse, New York 13210

The phosphorylation of cyclin D1 at threonine 286 by glycogen synthase kinase 3 (GSK3) has been shown to be required for the ubiquitination and nuclear export of cyclin D1 and its subsequent degradation in the proteasome. The mutation of the nearby residue, threonine 288, to nonphosphorylatable alanine has also been shown to reduce the ubiquitination of cyclin D1, suggesting that phosphorylation at threonine 288 may also lead to degradation of cyclin D1. We now demonstrate that the G₀/G₁-active arginine-directed protein kinase Mirk/dyrk1B binds to cyclin D1 and phosphorylates cyclin D1 at threonine 288 in vivo and that the cyclin D1-T288A construct is more stable than wild-type cyclin D1. Transient overexpression of Mirk in nontransformed Mv1Lu lung epithelial cells blocked cells in G₀/G₁. Depletion of endogenous Mirk by RNA interference increased cyclin D1 protein levels but not mRNA levels, indicating that Mirk destabilizes cyclin D1 protein. Destabilization was confirmed by induction of a stable Mirk transfectant of Mv1Lu cells, which blocked cell migration (Zou, Y., Lim, S., Lee, K., Deng, X., and Friedman, E. (2003) J. Biol. Chem. 278, 49573-49581), and caused a decrease in the half-life of endogenous cyclin D1, concomitant with an increase in Mirk expression. In vitro cyclin D1 was phosphorylated in an additive fashion by Mirk and GSK3. Mirk-phosphorylated cyclin D1 mutated at the GSK3 phosphorylation site and was capable of phosphorylating cyclin D1 in the presence of the GSK3 inhibitor LiCl. Mirk may function together with GSK3 to assist cell arrest in G₀/G₁ by destabilizing cyclin D1.

Received for publication, March 18, 2004

^* This work was supported by Public Health Service Award RO1 CA67405 (to E. F.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dept. of Pathology, Upstate Medical University, 2303 Weiskotten Hall, 750 E. Adams St., Syracuse, NY 13210. Tel.: 315-464-7138; Fax: 315-464-8419; E-mail: friedmae{at}upstate.edu.

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