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J. Biol. Chem., Vol. 279, Issue 27, 28028-28035, July 2, 2004

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Conditional Expression of 15-Lipoxygenase-1 Inhibits the Selenoenzyme Thioredoxin Reductase

MODULATION OF SELENOPROTEINS BY LIPOXYGENASE ENZYMES^*

Margaret K. Yu, Philip J. Moos, Pamela Cassidy¶, Mark Wade, and F. A. Fitzpatrick¶||

From the Departments of Internal Medicine, Oncological Science, and ^¶Medicinal Chemistry, The Huntsman Cancer Institute, University of Utah Health Sciences, Salt Lake City, Utah 84112-0555

The selenoenzyme thioredoxin reductase regulates redox-sensitive proteins involved in inflammation and carcinogenesis, including ribonucleotide reductase, p53, NFB, and others. Little is known about endogenous cellular factors that modulate thioredoxin reductase activity. Here we report that several metabolites of 15-lipoxygenase-1 inhibit purified thioredoxin reductase in vitro. 15(S)-Hydroperoxy-5,8,11-cis-13-trans-eicosatetraenoic acid, a metastable hydroperoxide generated by 15-lipoxygenase-1, and 4-hydroxy-2-nonenal, its non-enzymatic rearrangement product inhibit thioredoxin reductase with IC₅₀ = 13 ± 1.5 µM and 1 ± 0.2 µM, respectively. Endogenously generated metabolites of 15-lipoxygenase-1 also inhibit thioredoxin reductase in HEK-293 cells that harbor a 15-LOX-1 gene under the control of an inducible promoter complex. Conditional, highly selective induction of 15-lipoxygenase-1 caused an inhibition of ribonucleotide reductase activity, cell cycle arrest in G₁, impairment of anchorage-independent growth, and accumulation of the pro-apoptotic protein BAX. All of these responses are consistent with inhibition of thioredoxin reductase via 15-lipoxygenase-1 overexpression. In contrast, metabolites of 5-lipoxygenase were poor inhibitors of isolated thioredoxin reductase, and the overexpression of 5-lipoxygenase did not inhibit thioredoxin reductase or cause a G cell cycle arrest. The influences of 15-lipoxygenase-1 on ¹inflammation, cell growth, and survival may be attributable, in part, to inhibition of thioredoxin reductase and several redox-sensitive processes subordinate to thioredoxin reductase.

Received for publication, December 19, 2003 , and in revised form, April 26, 2004.

^* This work was supported by the Huntsman Cancer Foundation and by United States Public Health Services Grant R01 AI26730 (to F. A. F.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^|| To whom correspondence should be addressed. Tel.: 801-581-6204; Fax: 801-585-0101; E-mail: frank.fitzpatrick{at}hci.utah.edu.

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