HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 27, 28330-28338, July 2, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/27/28330    most recent M401269200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yasumoto, K.-i. Articles by Hearing, V. J. Search for Related Content PubMed PubMed Citation Articles by Yasumoto, K.-i. Articles by Hearing, V. J. Social Bookmarking                      What's this?

Epitope Mapping of the Melanosomal Matrix Protein gp100 (PMEL17)

RAPID PROCESSING IN THE ENDOPLASMIC RETICULUM AND GLYCOSYLATION IN THE EARLY GOLGI COMPARTMENT^*

Ken-ichi Yasumoto, Hidenori Watabe, Julio C. Valencia, Tsuneto Kushimoto, Takeshi Kobayashi, Ettore Appella, and Vincent J. Hearing¶

From the Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892 and Exploratory Research for Advanced Technology, Japan Science and Technology Corporation, Nagoya 460-0012 Japan

Melanosomes, specific organelles produced only by melanocytes, undergo a unique maturation process that involves their transition form amorphous rounded vesicles to fibrillar ellipsoid organelles, during which they move from the perinuclear to the distal areas of the cells. This depends upon the trafficking and processing of gp100 (also known as Pmel17 and the silver protein), a protein of great interest, because it elicits immune responses in melanoma patients but in which specific function(s) remains elusive. In this study, we have used biochemical and immunochemical approaches to more critically assess the synthesis, processing, glycosylation, and trafficking of gp100. We now report that gp100 is processed and sorted in a manner distinct from other melanosomal proteins (such as tyrosinase, Tyrp1 and Dct) and is predominantly delivered directly to immature melanosomes following its rapid processing in the endoplasmic reticulum and cis-Golgi. Following its arrival, gp100 is cleaved at the amino and at the carboxyl termini in a series of specific steps that result in the reorganization of immature melanosomes to the fibrillar mature melanosomes. Once this structural reorganization occurs, melanogenic enzymes begin to be targeted to the melanosomes, which are then competent to synthesize melanin pigment.

Received for publication, February 4, 2004 , and in revised form, March 22, 2004.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed: Laboratory of Cell Biology, Bldg. 37, Rm. 1B25, NCI, National Institutes of Health, Bethesda, MD 20892. Tel.: 301-496-1564; Fax: 301-402-8787; E-mail: hearingv{at}nih.gov.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. P. Kummer, H. Maruyama, C. Huelsmann, S. Baches, S. Weggen, and E. H. Koo Formation of Pmel17 Amyloid Is Regulated by Juxtamembrane Metalloproteinase Cleavage, and the Resulting C-terminal Fragment Is a Substrate for {gamma}-Secretase J. Biol. Chem., January 23, 2009; 284(4): 2296 - 2306. [Abstract] [Full Text] [PDF]

				V. Robila, M. Ostankovitch, M. L. Altrich-VanLith, A. C. Theos, S. Drover, M. S. Marks, N. Restifo, and V. H. Engelhard MHC Class II Presentation of gp100 Epitopes in Melanoma Cells Requires the Function of Conventional Endosomes and Is Influenced by Melanosomes J. Immunol., December 1, 2008; 181(11): 7843 - 7852. [Abstract] [Full Text] [PDF]

				D. C. Harper, A. C. Theos, K. E. Herman, D. Tenza, G. Raposo, and M. S. Marks Premelanosome Amyloid-like Fibrils Are Composed of Only Golgi-processed Forms of Pmel17 That Have Been Proteolytically Processed in Endosomes J. Biol. Chem., January 25, 2008; 283(4): 2307 - 2322. [Abstract] [Full Text] [PDF]

				T. Kobayashi and V. J. Hearing Direct interaction of tyrosinase with Tyrp1 to form heterodimeric complexes in vivo J. Cell Sci., December 15, 2007; 120(24): 4261 - 4268. [Abstract] [Full Text] [PDF]

				T. Passeron, J. C. Valencia, C. Bertolotto, T. Hoashi, E. Le Pape, K. Takahashi, R. Ballotti, and V. J. Hearing SOX9 is a key player in ultraviolet B-induced melanocyte differentiation and pigmentation PNAS, August 28, 2007; 104(35): 13984 - 13989. [Abstract] [Full Text] [PDF]

				J. C. Valencia, F. Rouzaud, S. Julien, K. G. Chen, T. Passeron, Y. Yamaguchi, M. Abu-Asab, M. Tsokos, G. E. Costin, H. Yamaguchi, et al. Sialylated Core 1 O-Glycans Influence the Sorting of Pmel17/gp100 and Determine Its Capacity to Form Fibrils J. Biol. Chem., April 13, 2007; 282(15): 11266 - 11280. [Abstract] [Full Text] [PDF]

				A. C. Theos, J. F. Berson, S. C. Theos, K. E. Herman, D. C. Harper, D. Tenza, E. V. Sviderskaya, M. L. Lamoreux, D. C. Bennett, G. Raposo, et al. Dual Loss of ER Export and Endocytic Signals with Altered Melanosome Morphology in the silver Mutation of Pmel17 Mol. Biol. Cell, August 1, 2006; 17(8): 3598 - 3612. [Abstract] [Full Text] [PDF]

				T. Hoashi, J. Muller, W. D. Vieira, F. Rouzaud, K. Kikuchi, K. Tamaki, and V. J. Hearing The Repeat Domain of the Melanosomal Matrix Protein PMEL17/GP100 Is Required for the Formation of Organellar Fibers J. Biol. Chem., July 28, 2006; 281(30): 21198 - 21208. [Abstract] [Full Text] [PDF]

				T. Hoashi, H. Watabe, J. Muller, Y. Yamaguchi, W. D. Vieira, and V. J. Hearing MART-1 Is Required for the Function of the Melanosomal Matrix Protein PMEL17/GP100 and the Maturation of Melanosomes J. Biol. Chem., April 8, 2005; 280(14): 14006 - 14016. [Abstract] [Full Text] [PDF]