HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 27, 28358-28366, July 2, 2004

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 279/27/28358    most recent M403202200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kasiviswanathan, R. Articles by Kelman, Z. Search for Related Content PubMed PubMed Citation Articles by Kasiviswanathan, R. Articles by Kelman, Z. Social Bookmarking                      What's this?

Biochemical Characterization of the Methanothermobacter thermautotrophicus Minichromosome Maintenance (MCM) Helicase N-terminal Domains^*

Rajesh Kasiviswanathan, Jae-Ho Shin, Eugene Melamud, and Zvi Kelman

From the University of Maryland Biotechnology Institute, Center for Advanced Research in Biotechnology, Rockville, Maryland 20850

Minichromosome maintenance helicases are ring-shaped complexes that play an essential role in archaeal and eukaryal DNA replication by separating the two strands of chromosomal DNA to provide the single-stranded substrate for the replicative polymerases. For the archaeal protein it was shown that the N-terminal portion of the protein, which is composed of domains A, B, and C, is involved in multimer formation and single-stranded DNA binding and may also play a role in regulating the helicase activity. Here, a detailed biochemical characterization of the N-terminal region of the Methanothermobacter thermautotrophicus minichromosome maintenance helicase is described. Using biochemical and biophysical analyses it is shown that domain C of the N-terminal portion, located adjacent to the helicase catalytic domains, is required for protein multimerization and that domain B is the main contact region with single-stranded DNA. It is also shown that although oligomerization is not essential for single-stranded DNA binding and ATPase activity, the presence of domain C is essential for helicase activity.

Received for publication, March 22, 2004

^* This work was supported by American Cancer Society Research Scholar Grant RSG-04-050-01-GMC. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains two supplemental figures.

To whom correspondence should be addressed: University of Maryland Biotechnology Institute, Center for Advanced Research in Biotechnology, 9600 Gudelsky Dr., Rockville, MD 20850. Tel.: 301-738-6294; Fax: 301-738-6255; E-mail: kelman{at}umbi.umd.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				W. Liu, B. Pucci, M. Rossi, F. M. Pisani, and R. Ladenstein Structural analysis of the Sulfolobus solfataricus MCM protein N-terminal domain Nucleic Acids Res., June 1, 2008; 36(10): 3235 - 3243. [Abstract] [Full Text] [PDF]

				N. Sakakibara, R. Kasiviswanathan, E. Melamud, M. Han, F. P. Schwarz, and Z. Kelman Coupling of DNA binding and helicase activity is mediated by a conserved loop in the MCM protein Nucleic Acids Res., March 27, 2008; 36(4): 1309 - 1320. [Abstract] [Full Text] [PDF]

				B. Pucci, M. De Felice, M. Rocco, F. Esposito, M. De Falco, L. Esposito, M. Rossi, and F. M. Pisani Modular Organization of the Sulfolobus solfataricus Mini-chromosome Maintenance Protein J. Biol. Chem., April 27, 2007; 282(17): 12574 - 12582. [Abstract] [Full Text] [PDF]

				E. R. Barry, A. T. McGeoch, Z. Kelman, and S. D. Bell Archaeal MCM has separable processivity, substrate choice and helicase domains Nucleic Acids Res., February 16, 2007; 35(3): 988 - 998. [Abstract] [Full Text] [PDF]

				R. Kasiviswanathan, J.-H. Shin, and Z. Kelman Interactions between the archaeal Cdc6 and MCM proteins modulate their biochemical properties Nucleic Acids Res., September 8, 2005; 33(15): 4940 - 4950. [Abstract] [Full Text] [PDF]

				A. V. Mitkova, E. E. Biswas-Fiss, and S. B. Biswas Modulation of DNA Synthesis in Saccharomyces cerevisiae Nuclear Extract by DNA Polymerases and the Origin Recognition Complex J. Biol. Chem., February 25, 2005; 280(8): 6285 - 6292. [Abstract] [Full Text] [PDF]

				B. Pucci, M. De Felice, M. Rossi, S. Onesti, and F. M. Pisani Amino Acids of the Sulfolobus solfataricus Mini-chromosome Maintenance-like DNA Helicase Involved in DNA Binding/Remodeling J. Biol. Chem., November 19, 2004; 279(47): 49222 - 49228. [Abstract] [Full Text] [PDF]