| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 279, Issue 28, 28862-28872, July 9, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Biology, York University, Toronto, Ontario M3J 1P3, Canada
Tomato bushy stunt virus (TBSV) is the prototypical member of the genus Tombusvirus in the family Tombusviridae. The (+)-strand RNA genome of TBSV lacks both a 5' cap and a 3' poly(A) tail and instead contains a 3'-terminal RNA sequence that acts as a cap-independent translational enhancer (3' CITE). In this study, we have determined the RNA secondary structure of the translation-specific central segment of the 3' CITE, termed region 3.5 (R3.5). MFOLD structural modeling combined with solution structure mapping and comparative sequence analysis indicate that R3.5 adopts a branched structure that contains three major helices. Deletion and substitution studies revealed that two of these extended stem-loop (SL) structures are essential for 3' CITE activity in vivo. In particular, the terminal loop of one of these SLs, SL-B, was found to be critical for translation. Compensatory mutational analysis showed that SL-B functions by base pairing with another SL, SL3, in the 5' untranslated region of the TBSV genome. Thus, efficient translation of TBSV mRNA in vivo requires a 5'-3' RNA-RNA interaction that effectively circularizes the message. Similar types of interactions are also predicted to occur in TBSV subgenomic mRNAs between their 5' untranslated regions and the 3' CITE, and both genomic and subgenomic 5'-3' interactions are well conserved in all members of the genus Tombusvirus. In addition, a survey of other genera in Tombusviridae revealed the potential for similar 5'-3' RNA-RNA-based interactions in their viral mRNAs, suggesting that this mechanism extends throughout this large virus family.
Received for publication, February 4, 2004 , and in revised form, April 21, 2004.
* This work was supported in part by NSERC and PREA. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Supported by a NSERC postgraduate scholarship.
To whom correspondence should be addressed: Dept. of Biology, York University, 4700 Keele St., Toronto, Ontario M3J 1P3, Canada. Tel.: 416-736-2100 (ext. 40890 or 70352); Fax: 416-736-5698; E-mail: kawhite{at}yorku.ca.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Treder, E. L. Pettit Kneller, E. M. Allen, Z. Wang, K. S. Browning, and W. A. Miller The 3' cap-independent translation element of Barley yellow dwarf virus binds eIF4F via the eIF4G subunit to initiate translation RNA, January 1, 2008; 14(1): 134 - 147. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-X. Lin, W. Xu, and K. A. White A Multicomponent RNA-Based Control System Regulates Subgenomic mRNA Transcription in a Tombusvirus J. Virol., March 1, 2007; 81(5): 2429 - 2439. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Karetnikov and K. Lehto The RNA2 5' leader of Blackcurrant reversion virus mediates efficient in vivo translation through an internal ribosomal entry site mechanism J. Gen. Virol., January 1, 2007; 88(1): 286 - 297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Na, M. R. Fabian, and K. A. White Conformational organization of the 3' untranslated region in the tomato bushy stunt virus genome RNA, December 1, 2006; 12(12): 2199 - 2210. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Serrano, M. R. Pulido, M. Saiz, and E. Martinez-Salas The 3' end of the foot-and-mouth disease virus genome establishes two distinct long-range RNA-RNA interactions with the 5' end region. J. Gen. Virol., October 1, 2006; 87(Pt 10): 3013 - 3022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Yuan, Y. Cao, D. Xi, L. Guo, C. Han, D. Li, Y. Zhai, and J. Yu Analysis of the subgenomic RNAs and the small open reading frames of Beet black scorch virus. J. Gen. Virol., October 1, 2006; 87(Pt 10): 3077 - 3086. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Rakotondrafara, C. Polacek, E. Harris, and W. A. Miller Oscillating kissing stem-loop interactions mediate 5' scanning-dependent translation by a viral 3'-cap-independent translation element RNA, October 1, 2006; 12(10): 1893 - 1906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Fabian and K. A. White Analysis of a 3'-translation enhancer in a tombusvirus: A dynamic model for RNA-RNA interactions of mRNA termini RNA, July 1, 2006; 12(7): 1304 - 1314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Mizumoto, H.-o. Iwakawa, M. Kaido, K. Mise, and T. Okuno Cap-Independent Translation Mechanism of Red Clover Necrotic Mosaic Virus RNA2 Differs from That of RNA1 and Is Linked to RNA Replication. J. Virol., April 1, 2006; 80(8): 3781 - 3791. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. W. McCartney, J. S. Greenwood, M. R. Fabian, K. A. White, and R. T. Mullen Localization of the Tomato Bushy Stunt Virus Replication Protein p33 Reveals a Peroxisome-to-Endoplasmic Reticulum Sorting Pathway PLANT CELL, December 1, 2005; 17(12): 3513 - 3531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Raman and D. A. Brian Stem-Loop IV in the 5' Untranslated Region Is a cis-Acting Element in Bovine Coronavirus Defective Interfering RNA Replication J. Virol., October 1, 2005; 79(19): 12434 - 12446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Monkewich, H.-X. Lin, M. R. Fabian, W. Xu, H. Na, D. Ray, O. A. Chernysheva, P. D. Nagy, and K. A. White The p92 Polymerase Coding Region Contains an Internal RNA Element Required at an Early Step in Tombusvirus Genome Replication J. Virol., April 15, 2005; 79(8): 4848 - 4858. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
