HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 29, 30158-30167, July 16, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/29/30158    most recent M403343200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rouanet, C. Articles by Nasser, W. Search for Related Content PubMed PubMed Citation Articles by Rouanet, C. Articles by Nasser, W. Social Bookmarking                      What's this?

Definition of a Consensus DNA-binding Site for PecS, a Global Regulator of Virulence Gene Expression in Erwinia chrysanthemi and Identification of New Members of the PecS Regulon^*

Carine Rouanet, Sylvie Reverchon, Dmitry A. Rodionov, and William Nasser¶

From the Unité de Microbiologie et Génétique CNRS-Institut National des Sciences Appliquées-Université Claude Bernard Lyon, Unité Mixte de Recherche 5122 Université Claude Bernard Lyon, 69622 Villeurbanne Cedex, France

In Erwinia chrysanthemi, production of pectic enzymes is modulated by a complex network involving several regulators. One of them, PecS, which belongs to the MarR family, also controls the synthesis of various other virulence factors, such as cellulases and indigoidine. Here, the PecS consensus-binding site is defined by combining a systematic evolution of ligands by an exponential enrichment approach and mutational analyses. The consensus consists of a 23-base pair palindromic-like sequence (C_–11G_–10A_–9N_–8W_–7T_–6C_–5G_–4T_–3A_–2)T_–1A₀T₁(T₂A₃C₄G₅A₆N₇N₈N₉C₁₀G₁₁). Mutational experiments revealed that (i) the palindromic organization is required for the binding of PecS, (ii) the very conserved part of the consensus (–6 to 6) allows for a specific interaction with PecS, but the presence of the relatively degenerated bases located apart significantly increases PecS affinity, (iii) the four bases G, A, T, and C are required for efficient binding of PecS, and (iv) the presence of several binding sites on the same promoter increases the affinity of PecS. This consensus is detected in the regions involved in PecS binding on the previously characterized target genes. This variable consensus is in agreement with the observation that the members of the MarR family are able to bind various DNA targets as dimers by means of a winged helix DNA-binding motif. Binding of PecS on a promoter region containing the defined consensus results in a repression of gene transcription in vitro. Preliminary scanning of the E. chrysanthemi genome sequence with the consensus revealed the presence of strong PecS-binding sites in the intergenic region between fliE and fliFGHIJKLMNOPQR which encode proteins involved in the biogenesis of flagellum. Accordingly, PecS directly represses fliE expression. Thus, PecS seems to control the synthesis of virulence factors required for the key steps of plant infection.

Received for publication, March 25, 2004

^* This work was supported by grants from the Centre National de la Recherche Scientifique, from the Ministère Délégué à la Recherche et aux Nouvelles Technologies, and from Programme de Microbiologie 2003 (ACIM-2-17). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Present address: Mécanisme Moléculaire de la Pathogénie Bactérienne Unité, INSERM 629, Institut Pasteur de Lille, 1 Rue du Prof. Calmette, 59019 Lille Cedex, France.

Working visit to Unité de Microbiologie et Génétique CNRS-Institut National des Sciences Appliquées-Université Claude Bernard Lyon was supported by an exchange grant within the European Science Foundation Programme on Integrated Approaches for Functional Genomics. Present address: State Scientific Center GosNII Genetica, Moscow 117545, Russia.

^¶ To whom correspondence should be addressed. Tel.: 33-4-7243-2695; Fax: 33-4-7243-1584; E-mail: William.nasser{at}insa-lyon.fr.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				F. Hommais, C. Oger-Desfeux, F. Van Gijsegem, S. Castang, S. Ligori, D. Expert, W. Nasser, and S. Reverchon PecS Is a Global Regulator of the Symptomatic Phase in the Phytopathogenic Bacterium Erwinia chrysanthemi 3937 J. Bacteriol., November 15, 2008; 190(22): 7508 - 7522. [Abstract] [Full Text] [PDF]

				G. Condemine and A. Ghazi Differential Regulation of Two Oligogalacturonate Outer Membrane Channels, KdgN and KdgM, of Dickeya dadantii (Erwinia chrysanthemi) J. Bacteriol., August 15, 2007; 189(16): 5955 - 5962. [Abstract] [Full Text] [PDF]

				N. H. Bergman, E. C. Anderson, E. E. Swenson, B. K. Janes, N. Fisher, M. M. Niemeyer, A. D. Miyoshi, and P. C. Hanna Transcriptional Profiling of Bacillus anthracis during Infection of Host Macrophages Infect. Immun., July 1, 2007; 75(7): 3434 - 3444. [Abstract] [Full Text] [PDF]

				A. Brencic and S. C. Winans Detection of and Response to Signals Involved in Host-Microbe Interactions by Plant-Associated Bacteria Microbiol. Mol. Biol. Rev., March 1, 2005; 69(1): 155 - 194. [Abstract] [Full Text] [PDF]