HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 30, 31973-31982, July 23, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/30/31973    most recent M404410200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cherukuri, A. Articles by Pierce, S. K. Search for Related Content PubMed PubMed Citation Articles by Cherukuri, A. Articles by Pierce, S. K. Social Bookmarking                      What's this?

B Cell Signaling Is Regulated by Induced Palmitoylation of CD81^*

Anu Cherukuri, Robert H. Carter, Stephen Brooks¶, William Bornmann||**, Ronald Finn||, Cynthia S. Dowd, and Susan K. Pierce

From the Laboratory of Immunogenetics, NIAID, National Institutes of Health, Rockville, Maryland 20852, the Department of Medicine and Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, and the ^||Memorial Sloan Kettering Cancer Center, Radiochemistry Core Facility, New York, New York 10021

Signaling through the B cell antigen receptor (BCR) is amplified and prolonged by coligation of the BCR to the CD19/CD21/CD81 coreceptor complex. Coligation is induced during immune responses by the simultaneous binding of complement-tagged antigens to the complement receptor, CD21, and to the BCR. Enhanced signaling is due in part to the ability of the CD19/CD21/CD81 complex to stabilize the BCR in sphingolipid- and cholesterol-rich membrane microdomains termed lipid rafts. The tetraspanin CD81 is essential for the raft-stabilizing function of the coreceptor. Here we show that coligation of the BCR and the CD19/CD21/CD81 complex leads to selective, rapid, and reversible palmitoylation of CD81 and that palmitoylation is necessary for the raft stabilizing function of the CD19/CD21/CD81 complex. Inducible palmitoylation may represent a novel mechanism by which tetraspanins function to facilitate lipid raft-dependent receptor signaling.

Received for publication, April 21, 2004 , and in revised form, May 24, 2004.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ Current address: Molecular Inflammation Section, NIAMS, NIH, Bethesda, MD 20892.

^** Current address: M. D. Anderson Cancer Center, Houston, TX 77030.

To whom correspondence should be addressed: Laboratory of Immunogenetics, NIAID/NIH/Twinbrook II, 12441 Parklawn Dr., Rm. 200B, MSC 8180, Rockville, MD 20852. Tel.: 301-496-9589; Fax: 301-402-0259; E-mail: spierce{at}nih.gov.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. Arduise, T. Abache, L. Li, M. Billard, A. Chabanon, A. Ludwig, P. Mauduit, C. Boucheix, E. Rubinstein, and F. Le Naour Tetraspanins Regulate ADAM10-Mediated Cleavage of TNF-{alpha} and Epidermal Growth Factor J. Immunol., November 15, 2008; 181(10): 7002 - 7013. [Abstract] [Full Text] [PDF]

				C. Sharma, X. H. Yang, and M. E. Hemler DHHC2 Affects Palmitoylation, Stability, and Functions of Tetraspanins CD9 and CD151 Mol. Biol. Cell, August 1, 2008; 19(8): 3415 - 3425. [Abstract] [Full Text] [PDF]

				A. Serra, S. Nuti, S. Tavarini, C. Sammicheli, D. Rosa, G. Saletti, E. Soldaini, S. Abrignani, and A. Wack Coligation of the Hepatitis C Virus Receptor CD81 with CD28 Primes Naive T Lymphocytes to Acquire Type 2 Effector Function J. Immunol., July 1, 2008; 181(1): 174 - 185. [Abstract] [Full Text] [PDF]

				D. A. Brown Lipid Rafts, Detergent-Resistant Membranes, and Raft Targeting Signals. Physiology, December 1, 2006; 21(6): 430 - 439. [Abstract] [Full Text] [PDF]

				H. W. Sohn, P. Tolar, T. Jin, and S. K. Pierce Fluorescence resonance energy transfer in living cells reveals dynamic membrane changes in the initiation of B cell signaling PNAS, May 23, 2006; 103(21): 8143 - 8148. [Abstract] [Full Text] [PDF]

				X. H. Yang, O. V. Kovalenko, T. V. Kolesnikova, M. M. Andzelm, E. Rubinstein, J. L. Strominger, and M. E. Hemler Contrasting Effects of EWI Proteins, Integrins, and Protein Palmitoylation on Cell Surface CD9 Organization J. Biol. Chem., May 5, 2006; 281(18): 12976 - 12985. [Abstract] [Full Text] [PDF]

				C. Bertaux and T. Dragic Different domains of CD81 mediate distinct stages of hepatitis C virus pseudoparticle entry. J. Virol., May 1, 2006; 80(10): 4940 - 4948. [Abstract] [Full Text] [PDF]

				T. Shoham, R. Rajapaksa, C.-C. Kuo, J. Haimovich, and S. Levy Building of the Tetraspanin Web: Distinct Structural Domains of CD81 Function in Different Cellular Compartments Mol. Cell. Biol., February 15, 2006; 26(4): 1373 - 1385. [Abstract] [Full Text] [PDF]

				A. Jimenez-Perianez, G. Ojeda, G. Criado, A. Sanchez, E. Pini, J. Madrenas, J. M. Rojo, and P. Portoles Complement regulatory protein Crry/p65-mediated signaling in T lymphocytes: role of its cytoplasmic domain and partitioning into lipid rafts J. Leukoc. Biol., December 1, 2005; 78(6): 1386 - 1396. [Abstract] [Full Text] [PDF]

				B. J. Chen, M. Takeda, and R. A. Lamb Influenza Virus Hemagglutinin (H3 Subtype) Requires Palmitoylation of Its Cytoplasmic Tail for Assembly: M1 Proteins of Two Subtypes Differ in Their Ability To Support Assembly J. Virol., November 1, 2005; 79(21): 13673 - 13684. [Abstract] [Full Text] [PDF]

				F. Martin, D. M. Roth, D. A. Jans, C. W. Pouton, L. J. Partridge, P. N. Monk, and G. W. Moseley Tetraspanins in Viral Infections: a Fundamental Role in Viral Biology? J. Virol., September 1, 2005; 79(17): 10839 - 10851. [Full Text] [PDF]

				Q.-R. Liu, T. Drgon, D. Walther, C. Johnson, O. Poleskaya, J. Hess, and G. R. Uhl Pooled association genome scanning: Validation and use to identify addiction vulnerability loci in two samples PNAS, August 16, 2005; 102(33): 11864 - 11869. [Abstract] [Full Text] [PDF]

				S. Levy and T. Shoham Protein-Protein Interactions in the Tetraspanin Web Physiology, August 1, 2005; 20(4): 218 - 224. [Abstract] [Full Text] [PDF]

				K. K. Stein, P. Primakoff, and D. Myles Sperm-egg fusion: events at the plasma membrane J. Cell Sci., December 15, 2004; 117(26): 6269 - 6274. [Abstract] [Full Text] [PDF]

				S. Schuck and K. Simons Polarized sorting in epithelial cells: raft clustering and the biogenesis of the apical membrane J. Cell Sci., December 1, 2004; 117(25): 5955 - 5964. [Abstract] [Full Text] [PDF]