HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 31, 32643-32650, July 30, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/31/32643    most recent M313247200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Aoki, M. Articles by Tohyama, M. Search for Related Content PubMed PubMed Citation Articles by Aoki, M. Articles by Tohyama, M. Social Bookmarking                      What's this?

EphA Receptors Direct the Differentiation of Mammalian Neural Precursor Cells through a Mitogen-activated Protein Kinase-dependent Pathway^*

Miwa Aoki, Toshihide Yamashita¶, and Masaya Tohyama

From the Department of Neurobiology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan and the Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan

Ephrins are cell surface-associated ligands for Eph receptor tyrosine kinases and are implicated in repulsive axon guidance and cell migration. EphA2, 3, and 4 receptors and one of their cognate ligands, ephrin-A2, are expressed by cells in the subventricular zone and ganglionic eminence of the embryonic day 14.5 telencephalon and by neural precursor cells in vitro. Activation of EphA receptors in dissociated neural precursor cells in vitro facilitates the commitment to neuronal fates. The majority of ephrin-A1-induced neurons is immunoreactive for tyrosine hydroxylase. Blocking the signal by the extracellular domain of EphA in forebrain slices results in a decrease in neurogenesis. Extracellular signal-regulated kinase is activated by the ligand binding to EphA receptors and is involved in the neurogenesis through EphA receptors. Rap1, but not Ras, is activated in response to ephrin-A1. Our results identify EphA receptors as positive regulators of the mitogen-activated protein kinase pathway that exerts neurogenesis of neural precursor cells from the developing central nervous system.

Received for publication, December 4, 2003 , and in revised form, May 11, 2004.

^* This work was partly supported by the 21st Century Center of Excellence Program, 32 Grant-in-aid 14657343 from the Ministry of Education, Culture, Sports and Technology of Japan, and Research Grant 15A-2 for Nervous and Mental Disorders from the Ministry of Health, Labour and Welfare. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed: Dept. of Neurobiology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan. Tel.: 81-43-2262024; Fax: 81-43-2262025; E-mail: t-yamashita{at}faculty.chiba-u.jp.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. Fukai, H. Yokote, R. Yamanaka, T. Arao, K. Nishio, and T. Itakura EphA4 promotes cell proliferation and migration through a novel EphA4-FGFR1 signaling pathway in the human glioma U251 cell line Mol. Cancer Ther., September 1, 2008; 7(9): 2768 - 2778. [Abstract] [Full Text] [PDF]

				R. Qiu, X. Wang, A. Davy, C. Wu, K. Murai, H. Zhang, J. G. Flanagan, P. Soriano, and Q. Lu Regulation of neural progenitor cell state by ephrin-B J. Cell Biol., June 16, 2008; 181(6): 973 - 983. [Abstract] [Full Text] [PDF]

				M. Richter, K. K. Murai, C. Bourgin, D. T. Pak, and E. B. Pasquale The EphA4 Receptor Regulates Neuronal Morphology through SPAR-Mediated Inactivation of Rap GTPases J. Neurosci., December 19, 2007; 27(51): 14205 - 14215. [Abstract] [Full Text] [PDF]

				V. Devgan, B.-C. Nguyen, H. Oh, and G. P. Dotto p21WAF1/Cip1 Suppresses Keratinocyte Differentiation Independently of the Cell Cycle through Transcriptional Up-regulation of the IGF-I Gene J. Biol. Chem., October 13, 2006; 281(41): 30463 - 30470. [Abstract] [Full Text] [PDF]

				J. J. Munoz, D. Alfaro, J. Garcia-Ceca, L. M. Alonso-C, E. Jimenez, and A. Zapata Thymic Alterations in EphA4-Deficient Mice J. Immunol., July 15, 2006; 177(2): 804 - 813. [Abstract] [Full Text] [PDF]

				A. K. Jassen, H. Yang, G. M. Miller, E. Calder, and B. K. Madras Receptor Regulation of Gene Expression of Axon Guidance Molecules: Implications for Adaptation Mol. Pharmacol., July 1, 2006; 70(1): 71 - 77. [Abstract] [Full Text] [PDF]

				M. Koolpe, R. Burgess, M. Dail, and E. B. Pasquale EphB Receptor-binding Peptides Identified by Phage Display Enable Design of an Antagonist with Ephrin-like Affinity J. Biol. Chem., April 29, 2005; 280(17): 17301 - 17311. [Abstract] [Full Text] [PDF]