HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 32, 33199-33205, August 6, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/32/33199    most recent M402943200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Turner, L. J. Articles by Hall, A. Search for Related Content PubMed PubMed Citation Articles by Turner, L. J. Articles by Hall, A. Social Bookmarking                      What's this?

The Activity of the Plexin-A1 Receptor Is Regulated by Rac^*

Laura J. Turner, Sarah Nicholls, and Alan Hall

From the MRC Laboratory for Molecular Cell Biology and Cell Biology Unit, Department of Biochemistry and Molecular Biology, Cancer Research UK, Oncogene and Signal Transduction Group, University College London, Gower Street, London, WC1E 6BT, United Kingdom

Plexins constitute a large family of transmembrane proteins that act as receptors for the semaphorin family of ligands. They are best known for their role in growth cone guidance, although they also are widely expressed outside the nervous system. Plexins are thought to control axon guidance by modifying the growth cone cytoskeleton, and Rho GTPases have been strongly implicated in this response. However, the exact contribution of Rho proteins is unclear. Sema3A/Plexin-A1-induced growth cone collapse, for example, requires Rac activity, which is a surprising result given that this GTPase is usually associated with membrane protrusions. We show here that Sema3A-induced collapse of COS-7 cells expressing Plexin-A1 also requires Rac but not Rho activity and that the cytoplasmic tail of Plexin-A1 interacts directly with activated Rac. However, collapse induced by a constitutively activated version of Plexin-A1 does not require Rac. We propose a novel function for Rac, namely that it acts upstream of Plexin-A1 during semaphoring-induced collapse, to regulate the activity of the receptor.

Received for publication, March 16, 2004 , and in revised form, June 7, 2004.

^* The work was supported generously by a program grant from Cancer Research UK. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Recipient of an MRC Ph.D. fellowship.

To whom correspondence should be addressed. Tel.: 44-20-7679-7909; Fax: 44-20-7679-7804; E-mail: alan.hall{at}ucl.ac.uk.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. Nukazuka, H. Fujisawa, T. Inada, Y. Oda, and S. Takagi Semaphorin controls epidermal morphogenesis by stimulating mRNA translation via eIF2{alpha} in Caenorhabditis elegans Genes & Dev., April 15, 2008; 22(8): 1025 - 1036. [Abstract] [Full Text] [PDF]

				E. F. Schmidt, S.-O. Shim, and S. M. Strittmatter Release of MICAL Autoinhibition by Semaphorin-Plexin Signaling Promotes Interaction with Collapsin Response Mediator Protein J. Neurosci., February 27, 2008; 28(9): 2287 - 2297. [Abstract] [Full Text] [PDF]

				Y. Tong, P. Chugha, P. K. Hota, R. S. Alviani, M. Li, W. Tempel, L. Shen, H.-W. Park, and M. Buck Binding of Rac1, Rnd1, and RhoD to a Novel Rho GTPase Interaction Motif Destabilizes Dimerization of the Plexin-B1 Effector Domain J. Biol. Chem., December 21, 2007; 282(51): 37215 - 37224. [Abstract] [Full Text] [PDF]

				K. E. Waimey and H.-J. Cheng Axon Pruning and Synaptic Development: How Are They per-Plexin? Neuroscientist, October 1, 2006; 12(5): 398 - 409. [Abstract] [PDF]

				H. Togashi, E. F. Schmidt, and S. M. Strittmatter RanBPM contributes to Semaphorin3A signaling through plexin-A receptors. J. Neurosci., May 3, 2006; 26(18): 4961 - 4969. [Abstract] [Full Text] [PDF]

				S. Woo and T. M. Gomez Rac1 and RhoA Promote Neurite Outgrowth through Formation and Stabilization of Growth Cone Point Contacts J. Neurosci., February 1, 2006; 26(5): 1418 - 1428. [Abstract] [Full Text] [PDF]

				D. Barberis, A. Casazza, R. Sordella, S. Corso, S. Artigiani, J. Settleman, P. M. Comoglio, and L. Tamagnone p190 Rho-GTPase activating protein associates with plexins and it is required for semaphorin signalling J. Cell Sci., October 15, 2005; 118(20): 4689 - 4700. [Abstract] [Full Text] [PDF]

				H. Kashiwagi, M. Shiraga, H. Kato, T. Kamae, N. Yamamoto, S. Tadokoro, Y. Kurata, Y. Tomiyama, and Y. Kanakura Negative regulation of platelet function by a secreted cell repulsive protein, semaphorin 3A Blood, August 1, 2005; 106(3): 913 - 921. [Abstract] [Full Text] [PDF]

				G. Dalpe, L. Brown, and J. G. Culotti Vulva morphogenesis involves attraction of plexin 1-expressing primordial vulva cells to semaphorin 1a sequentially expressed at the vulva midline Development, March 15, 2005; 132(6): 1387 - 1400. [Abstract] [Full Text] [PDF]

				M. Brown, T. Jacobs, B. Eickholt, G. Ferrari, M. Teo, C. Monfries, R. Z. Qi, T. Leung, L. Lim, and C. Hall {alpha}2-Chimaerin, Cyclin-Dependent Kinase 5/p35, and Its Target Collapsin Response Mediator Protein-2 Are Essential Components in Semaphorin 3A-Induced Growth-Cone Collapse J. Neurosci., October 13, 2004; 24(41): 8994 - 9004. [Abstract] [Full Text] [PDF]