HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 32, 33751-33758, August 6, 2004

This Article Full Text Full Text (PDF) All Versions of this Article: 279/32/33751    most recent M401616200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sinclair, J. F. Articles by O'Brien, A. D. Search for Related Content PubMed PubMed Citation Articles by Sinclair, J. F. Articles by O'Brien, A. D. Social Bookmarking                      What's this?

Intimin Types , , and Bind to Nucleolin with Equivalent Affinity but Lower Avidity than to the Translocated Intimin Receptor^*

James F. Sinclair and Alison D. O'Brien

From the Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814

The outer membrane adhesins of enteropathogenic Escherichia coli, Citrobacter rodentium, and enterohemorrhagic E. coli (EHEC) O157:H7 that mediate attach and efface intestinal lesions are classified as intimin , , and , respectively. Each of these intimin types binds to its cognate, bacterially encoded receptor (called Tir for translocated intimin receptor) to promote tight adherence of the organism to the host-cell plasma membrane. We previously reported that intimin of EHEC O157:H7 also bound to a eucaryotic receptor that we determined was nucleolin. The objective of this study was to investigate in vitro and in vivo the interactions of intimins , , and with nucleolin in the presence of Tir from EHEC O157:H7. Protein binding experiments demonstrated that intimin of types , , and bound nucleolin with similar affinity. Moreover, all three intimin types co-localized with regions of nucleolin expressed on the surface of HEp-2 cells. When intimin , , or bound to Tir in vitro, the intimin interaction with nucleolin was blocked. Both Tir and nucleolin accumulated beneath intimin-presenting bacteria that had attached to the surface of HEp-2 cells. Taken together, these findings suggest that nucleolin is involved in bacterial adherence promoted by all intimin types and that Tir and nucleolin compete for intimin during adherence.

Received for publication, February 13, 2004 , and in revised form, May 11, 2004.

^* This work was supported by National Institutes of Health Grant AI 20148-21 (to A. D. O.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dept. of Microbiology and Immunology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Rd., Bethesda, MD 20814. Tel.: 301-295-3419; Fax: 301-295-3773; E-mail: aobrien{at}usuhs.mil.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Karama and C. L. Gyles Characterization of Verotoxin-Encoding Phages from Escherichia coli O103:H2 Strains of Bovine and Human Origins Appl. Envir. Microbiol., August 15, 2008; 74(16): 5153 - 5158. [Abstract] [Full Text] [PDF]

				T. K. Hajra, P. K. Bag, S. C. Das, S. Mukherjee, A. Khan, and T. Ramamurthy Development of a Simple Latex Agglutination Assay for Detection of Shiga Toxin-Producing Escherichia coli (STEC) by Using Polyclonal Antibody against STEC Clin. Vaccine Immunol., May 1, 2007; 14(5): 600 - 604. [Abstract] [Full Text] [PDF]

				R. Mundy, S. Schuller, F. Girard, J. M. Fairbrother, A. D. Phillips, and G. Frankel Functional studies of intimin in vivo and ex vivo: implications for host specificity and tissue tropism Microbiology, April 1, 2007; 153(4): 959 - 967. [Abstract] [Full Text] [PDF]

				C. L. Gyles Shiga toxin-producing Escherichia coli: An overview J Anim Sci, March 1, 2007; 85(13_suppl): E45 - E62. [Abstract] [Full Text] [PDF]

				J. F. Sinclair, E. A. Dean-Nystrom, and A. D. O'Brien The Established Intimin Receptor Tir and the Putative Eucaryotic Intimin Receptors Nucleolin and {beta}1 Integrin Localize at or near the Site of Enterohemorrhagic Escherichia coli O157:H7 Adherence to Enterocytes In Vivo Infect. Immun., February 1, 2006; 74(2): 1255 - 1265. [Abstract] [Full Text] [PDF]

				I. Vlisidou, F. Dziva, R. M. La Ragione, A. Best, J. Garmendia, P. Hawes, P. Monaghan, S. A. Cawthraw, G. Frankel, M. J. Woodward, et al. Role of Intimin-Tir Interactions and the Tir-Cytoskeleton Coupling Protein in the Colonization of Calves and Lambs by Escherichia coli O157:H7 Infect. Immun., January 1, 2006; 74(1): 758 - 764. [Abstract] [Full Text] [PDF]

				F. Girard, I. Batisson, G. M. Frankel, J. Harel, and J. M. Fairbrother Interaction of Enteropathogenic and Shiga Toxin-Producing Escherichia coli and Porcine Intestinal Mucosa: Role of Intimin and Tir in Adherence Infect. Immun., September 1, 2005; 73(9): 6005 - 6016. [Abstract] [Full Text] [PDF]

				J. Garmendia, G. Frankel, and V. F. Crepin Enteropathogenic and Enterohemorrhagic Escherichia coli Infections: Translocation, Translocation, Translocation Infect. Immun., May 1, 2005; 73(5): 2573 - 2585. [Full Text] [PDF]

				R. K Shaw, J. Cleary, M. S. Murphy, G. Frankel, and S. Knutton Interaction of Enteropathogenic Escherichia coli with Human Intestinal Mucosa: Role of Effector Proteins in Brush Border Remodeling and Formation of Attaching and Effacing Lesions Infect. Immun., February 1, 2005; 73(2): 1243 - 1251. [Abstract] [Full Text] [PDF]

				A. G. Torres, X. Zhou, and J. B. Kaper Adherence of Diarrheagenic Escherichia coli Strains to Epithelial Cells Infect. Immun., January 1, 2005; 73(1): 18 - 29. [Full Text] [PDF]