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J. Biol. Chem., Vol. 279, Issue 34, 35384-35391, August 20, 2004

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Nuclear Export of the Yeast mRNA-binding Protein Nab2 Is Linked to a Direct Interaction with Gfd1 and to Gle1 Function^*

Mythili Suntharalingam, Abel R. Alcázar-Román, and Susan R. Wente

From the Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, Tennessee 37232

Nuclear export of mRNA is mediated by interactions between soluble factors and nuclear pore complex (NPC) proteins. In Saccharomyces cerevisiae, Nab2 is an essential RNA-binding protein that shuttles between the nucleus and cytoplasm. The mechanism for trafficking of Nab2-bound mRNA through the NPC has not been defined. Gle1 is also required for mRNA export, and Gle1 interactions with NPC proteins, the RNA helicase Dbp5, and Gfd1 have been reported. Here we report that Nab2, Gfd1, and Gle1 associate in a complex. By using immobilized recombinant Gfd1, Nab2 was isolated from total yeast lysate. A similar biochemical assay with immobilized recombinant Nab2 resulted in coisolation of Gfd1 and Gle1. A Nab2-Gfd1 complex was also identified by coimmunoprecipitation from yeast lysates. In vitro binding assays with recombinant proteins revealed a direct association between Nab2 and Gfd1, and two-hybrid assays delineated Gfd1 binding to the N-terminal Nab2 domain. This N-terminal Nab2 domain is distinct from its RNA binding domains suggesting Nab2 could bind Gfd1 and RNA simultaneously. As Nab2 export was blocked in a gle1 mutant at the restrictive temperature, we propose a model wherein Gfd1 serves as a bridging factor between Gle1 and Nab2-bound mRNA during export.

Received for publication, February 24, 2004 , and in revised form, June 10, 2004.

^* This work was supported by National Institutes of Health Grant GM51219 (to S. R. W.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Present address: Medical Scientist Training Program, Washington University School of Medicine, St. Louis, MO 63110.

To whom correspondence should be addressed: Dept. of Cell and Developmental Biology, Vanderbilt University Medical Center, 3120 MRBIII, 465 21st Ave. South, Nashville, TN 37232. Tel.: 615-936-3443; Fax: 615-936-3439; E-mail: susan.wente{at}vanderbilt.edu.

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