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J. Biol. Chem., Vol. 279, Issue 34, 35469-35478, August 20, 2004

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Gle2p Is Essential to Induce Adaptation of the Export of Bulk Poly(A)⁺ mRNA to Heat Shock in Saccharomyces cerevisiae^*

Shingo Izawa, Reiko Takemura, and Yoshiharu Inoue

From the Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan

The export of bulk poly(A)⁺ mRNA is blocked under heat-shocked (42 °C) conditions in Saccharomyces cerevisiae. We found that an mRNA export factor Gle2p rapidly dissociated from the nuclear envelope and diffused into the cytoplasm at 42 °C. However, in exponential phase cells pretreated with mild heat stress (37 °C for 1 h), Gle2p did not dissociate at 42 °C, and the export of bulk poly(A)⁺ mRNA continued. Cells in stationary phase also continued with the export of bulk poly(A)⁺ mRNA at 42 °C without the dissociation of Gle2p from the nuclear envelope. The dissociation of Gle2p was caused by increased membrane fluidity and correlated closely with blocking of the export of bulk poly(A)⁺ mRNA. Furthermore, the mutants gle2 and rip1 could not induce such an adaptation of the export of bulk poly(A)⁺ mRNA to heat shock. Our findings indicate that Gle2p plays a crucial role in mRNA export especially under heat-shocked conditions. Our findings also indicate that the nuclear pore complexes that Gle2p constitutes need to be stabilized for the adaptation and that the increased membrane integrity caused by treatment with mild heat stress or by survival in stationary phase is likely to contribute to the stabilization of the association between Gle2p and the nuclear pore complexes.

Received for publication, April 2, 2004 , and in revised form, June 18, 2004.

^* This work was supported by the Kato Bio Science Research Foundation, Bio-oriented Technology Research Advancement Institution (BRAIN), and Japanese Ministry of Education, Culture, Sports, Science, and Technology Grant 14035229. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan. Tel.: 81-774-38-3731; Fax: 81-774-33-3004; E-mail: izawa{at}kais.kais.kyoto-u.ac.jp.

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